Overview

Also Known As:Pep Boy, B6 Cd45.1

This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential Ptprc^a pan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprc^b (CD45.2 or Ly5.2) allele.

Genetic overview

Genetic Background	Generation

Ptprc^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	Ptprc	protein tyrosine phosphatase, receptor type, C

Pepc^b

Allele Type	Gene Symbol	Gene Name
Not Applicable	Pepc	peptidase C

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools

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Base Price

Starting at:

$56.64 Domestic price for female 3-week

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Details

Detailed Description

This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential pan leukocyte marker Ptprc^a, commonly known as CD45.1 or Ly5.1. Wild-type C57BL and BALB/c inbred strains express the Ptprc^b (CD45.2 or Ly5.2) allele.

The current gene symbol designation Ptprc instead of Cd45 or Ly5 is based on work by Charbonneau and colleagues who showed in humans that a protein-tyrosine phosphatase (human placental protein-tyrosine phosphatase 1B) is homologous to the CD45 protein. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth.

A spontaneous mutation in the Ncr1 gene is segregating in this strain (Jang et al. 2018 J Immunol 200:1982).

Chisolm et al. 2019 Immunity 51:155 characterized the SJL-derived genome surrounding the Ptprc^a allele on chromosome 1 using B6.SJL-CD45.1 mice obtained from Stock No. 002014. They identified a 37 Mb genomic interval of SJL genetic content including from Bcl2 to Glrx2 (Chr1:106,538,176-143,749,678; mm10 reference genome) - see Figure 4C (blue box) and Table S2 (the "Jax_B6.SJL-CD45.1 interval" tab). The publication describes how SJL genetic content in B6.SJL-CD45.1 mice may contribute to differences in amino acid content, H3K27Ac, gene regulation and protein expression in CD4+ Th1 cells. As such, it is recommended that researchers using Stock No. 002014 determine how/if any of the SJL genetic content may affect their specific experiments.

Development

The Ptprc^a allele and closely linked Pepc^b allele were serially backcrossed from SJL/J onto C57BL/6 with selection at each generation for Ptprc^a by serotyping (Shen et al., 1985). In 1990 this strain, then housed at Sloan Kettering, was imported into The Jackson Laboratory from Edward Boyse at generation N22. Since its arrival, the strain has been maintained by sibling inbreeding. In 2005 it reached generation N22F55.

A spontaneous mutation in the Ncr1 gene on Chromosome 7 is segregating in this strain (Jang et al. 2018 J Immunol 200:1982).

Chisolm et al. 2019 Immunity 51:155 characterized the SJL-derived genome surrounding the Ptprc^a allele on chromosome 1 using B6.SJL-CD45.1 mice obtained from Stock No. 002014. They identified a 37 Mb genomic interval of SJL genetic content including from Bcl2 to Glrx2 (Chr1:106,538,176-143,749,678; mm10 reference genome) - see Figure 4C (blue box) and Table S2 (the "Jax_B6.SJL-CD45.1 interval" tab). The publication describes how SJL genetic content in B6.SJL-CD45.1 mice may contribute to differences in amino acid content, H3K27Ac, gene regulation and protein expression in CD4+ Th1 cells. As such, it is recommended that researchers using Stock No. 002014 determine how/if any of the SJL genetic content may affect their specific experiments.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Janowska-Wieczorek A; Majka M; Kijowski J; Baj-Krzyworzeka M; Reca R; Turner AR; Ratajczak J; Emerson SG; Kowalska MA; Ratajczak MZ. 2001. Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood 98(10):3143-9PubMed: 11698303 MGI: J:109863
Schluns KS; Williams K; Ma A; Zheng XX; Lefrancois L. 2002. Cutting edge: requirement for IL-15 in the generation of primary and memory antigen-specific CD8 T cells. J Immunol 168(10):4827-31PubMed: 11994430 MGI: J:109854
Yang G; Hisha H; Cui Y; Fan T; Jin T; Li Q; Lian Z; Hosaka N; Li Y; Ikehara S. 2002. A new assay method for late CFU-S formation and long-term reconstituting activity using a small number of pluripotent hemopoietic stem cells. Stem Cells 20(3):241-8PubMed: 12004082 MGI: J:109853

View All References

Genetics

Ptprc^a

Allele Symbol: Ptprc^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	CD45.1; Ly5^a; Ptprc^SJL
Gene Symbol and Name	Ptprc, protein tyrosine phosphatase, receptor type, C
Gene Synonym(s)
Site of Expression	Widely expressed on all adaptive and innate immune cells.
Strain of Origin	Not Applicable
Chromosome	1
Molecular Note	Ptprc^a is found in strains SJL/J, STS/A, and DA. Ptprc^b is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485). Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341).

Pepc^b

Allele Symbol: Pepc^b

Allele Name	b variant
Allele Type	Not Applicable
Allele Synonym(s)	Pep3^b
Gene Symbol and Name	Pepc, peptidase C
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	1
Molecular Note	The Pepc gene controls electrophoretic variation of peptidase C (PEP-C; EC 3.4.11.-) of red blood cells. The allele Pepc^b determines a faster migrating band and is found in the CBA/J and A/He strains.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Genetics Research
  - Tissue/Cell Markers: B cell specific marker
  - Tissue/Cell Markers

Mammalian Phenotype Terms by Genotype

Phenotype Information

Body Weight Information - JAX^® Mice Strain B6.SJL-Ptprc^a Pepc^b/BoyJ (002014)
JAX^® Physiological Data Summary [pdf]

References

Janowska-Wieczorek A; Majka M; Kijowski J; Baj-Krzyworzeka M; Reca R; Turner AR; Ratajczak J; Emerson SG; Kowalska MA; Ratajczak MZ. 2001. Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood 98(10):3143-9PubMed: 11698303 MGI: J:109863
Schluns KS; Williams K; Ma A; Zheng XX; Lefrancois L. 2002. Cutting edge: requirement for IL-15 in the generation of primary and memory antigen-specific CD8 T cells. J Immunol 168(10):4827-31PubMed: 11994430 MGI: J:109854
Yang G; Hisha H; Cui Y; Fan T; Jin T; Li Q; Lian Z; Hosaka N; Li Y; Ikehara S. 2002. A new assay method for late CFU-S formation and long-term reconstituting activity using a small number of pluripotent hemopoietic stem cells. Stem Cells 20(3):241-8PubMed: 12004082 MGI: J:109853

Additional References

Charbonneau H; Tonks NK; Walsh KA; Fischer EH. 1988. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase. Proc Natl Acad Sci U S A 85(19):7182-6PubMed: 2845400 MGI: J:22340
Held W; Clevers H; Grosschedl R. 2003. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. Eur J Immunol 33(5):1393-8PubMed: 12731066 MGI: J:85394
Morse HC 3rd. 1992. Genetic nomenclature for loci controlling surface antigens of mouse hemopoietic cells [published erratum appears in J Immunol 1993 Mar 15;150(6):2563] J Immunol 149(10):3129-34PubMed: 1431091 MGI: J:3170
Morse HC 3rd; Shen FW; Hammerling U. 1987. Genetic nomenclature for loci controlling mouse lymphocyte antigens. Immunogenetics 25(2):71-8PubMed: 3817907 MGI: J:8603
Shen FW; Saga Y; Litman G; Freeman G; Tung JS; Cantor H; Boyse EA. 1985. Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A 82(21):7360-3PubMed: 3864163 MGI: J:8080

Additional - Pepc^b related

Additional - Ptprc^a related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Pyrosequencing:Ptprc

Breeding Considerations

This strain is a good breeder.

Additional Breeding and Husbandry Support

Mating System

Sibling x Sibling

Appearance

black
Related Genotype: a/a

Citation
When using the B6 Cd45.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002014 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

MP14 (Maximum)
RB11 (Maximum)
RB09 (Maximum)
EM04 (Maximum)

Pricing & Availability

Readily Available

Domestic

International

Live Mouse

Age Genotype Price

weeks

Cryorecovery - Pricing

Service/Product Description Price

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Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Phone: 207-288-6470

Email: TechTran@jax.org

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Also Known As:Pep Boy, B6 Cd45.1

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Ptprca

Allele Symbol: Ptprca

Pepcb

Allele Symbol: Pepcb

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Phenotype Information

References

Additional References

Additional - Pepcb related

Additional - Ptprca related

Genotyping Protocols

Breeding Considerations

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Ptprc^a

Allele Symbol: Ptprc^a

Pepc^b

Allele Symbol: Pepc^b

Additional - Pepc^b related

Additional - Ptprc^a related