This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential Ptprca pan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprcb (CD45.2 or Ly5.2) allele.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Ptprc | protein tyrosine phosphatase, receptor type, C |
Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Pepc | peptidase C |
This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential pan leukocyte marker Ptprca, commonly known as CD45.1 or Ly5.1. Wild-type C57BL and BALB/c inbred strains express the Ptprcb (CD45.2 or Ly5.2) allele.
The current gene symbol designation Ptprc instead of Cd45 or Ly5 is based on work by Charbonneau and colleagues who showed in humans that a protein-tyrosine phosphatase (human placental protein-tyrosine phosphatase 1B) is homologous to the CD45 protein. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth.
A spontaneous mutation in the Ncr1 gene is segregating in this strain (Jang et al. 2018 J Immunol 200:1982).
Chisolm et al. 2019 Immunity 51:155 characterized the SJL-derived genome surrounding the Ptprca allele on chromosome 1 using B6.SJL-CD45.1 mice obtained from Stock No. 002014. They identified a 37 Mb genomic interval of SJL genetic content including from Bcl2 to Glrx2 (Chr1:106,538,176-143,749,678; mm10 reference genome) - see Figure 4C (blue box) and Table S2 (the "Jax_B6.SJL-CD45.1 interval" tab). The publication describes how SJL genetic content in B6.SJL-CD45.1 mice may contribute to differences in amino acid content, H3K27Ac, gene regulation and protein expression in CD4+ Th1 cells. As such, it is recommended that researchers using Stock No. 002014 determine how/if any of the SJL genetic content may affect their specific experiments.
The Ptprca allele and closely linked Pepcb allele were serially backcrossed from SJL/J onto C57BL/6 with selection at each generation for Ptprca by serotyping (Shen et al., 1985). In 1990 this strain, then housed at Sloan Kettering, was imported into The Jackson Laboratory from Edward Boyse at generation N22. Since its arrival, the strain has been maintained by sibling inbreeding. In 2005 it reached generation N22F55.
A spontaneous mutation in the Ncr1 gene on Chromosome 7 is segregating in this strain (Jang et al. 2018 J Immunol 200:1982).
Chisolm et al. 2019 Immunity 51:155 characterized the SJL-derived genome surrounding the Ptprca allele on chromosome 1 using B6.SJL-CD45.1 mice obtained from Stock No. 002014. They identified a 37 Mb genomic interval of SJL genetic content including from Bcl2 to Glrx2 (Chr1:106,538,176-143,749,678; mm10 reference genome) - see Figure 4C (blue box) and Table S2 (the "Jax_B6.SJL-CD45.1 interval" tab). The publication describes how SJL genetic content in B6.SJL-CD45.1 mice may contribute to differences in amino acid content, H3K27Ac, gene regulation and protein expression in CD4+ Th1 cells. As such, it is recommended that researchers using Stock No. 002014 determine how/if any of the SJL genetic content may affect their specific experiments.
Allele Name | a variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | CD45.1; Ly5a; PtprcSJL |
Gene Symbol and Name | Ptprc, protein tyrosine phosphatase, receptor type, C |
Gene Synonym(s) | |
Site of Expression | Widely expressed on all adaptive and innate immune cells. |
Strain of Origin | Not Applicable |
Chromosome | 1 |
Molecular Note | Ptprca is found in strains SJL/J, STS/A, and DA. Ptprcb is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485). Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341). |
Allele Name | b variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | Pep3b |
Gene Symbol and Name | Pepc, peptidase C |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 1 |
Molecular Note | The Pepc gene controls electrophoretic variation of peptidase C (PEP-C; EC 3.4.11.-) of red blood cells. The allele Pepcb determines a faster migrating band and is found in the CBA/J and A/He strains. |
When using the B6 Cd45.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002014 in your Materials and Methods section.
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