The Ptprca allele and closely linked Pepcb allele were serially backcrossed from SJL/J onto C57BL/6 with selection at each generation for Ptprca by serotyping (Shen et al., 1985). In 1990 this strain, then housed at Sloan Kettering, was imported into The Jackson Laboratory from Edward Boyse at generation N22. Since its arrival, the strain has been maintained by sibling inbreeding. In 2005 it reached generation N22F55.
A spontaneous mutation in the Ncr1 gene was reported to be segregating in this strain (Jang et al. 2018 J Immunol 200:1982). As of September 2020, our live colony no longer has this spontaneous mutation.
Chisolm et al. 2019 Immunity 51:155 characterized the SJL-derived genome surrounding the Ptprca allele on chromosome 1 using B6.SJL-CD45.1 mice obtained from Stock No. 002014. They identified a 37 Mb genomic interval of SJL genetic content including from Bcl2 to Glrx2 (Chr1:106,538,176-143,749,678; mm10 reference genome) - see Figure 4C (blue box) and Table S2 (the "Jax_B6.SJL-CD45.1 interval" tab). The publication describes how SJL genetic content in B6.SJL-CD45.1 mice may contribute to differences in amino acid content, H3K27Ac, gene regulation and protein expression in CD4+ Th1 cells. As such, it is recommended that researchers using Stock No. 002014 determine how/if any of the SJL genetic content may affect their specific experiments.
Body Weight Information - JAX® Mice Strain B6.SJL-Ptprca Pepcb/BoyJ (002014)
