B6.SJL-Ptprc^a Pepc^b/BoyJ

Stock number: 002014
Product name: B6 CD45.1
Strain synonyms: Pep Boy
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential Ptprc^a pan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprc^b (CD45.2 or Ly5.2) allele.



A CRISPR-generated coisogenic C57BL/6J strain expressing CD45.1 is now available as Stock No. 033076.

Detailed description

This C57BL/6 congenic strain is used widely in transplant studies because it carries the differential pan leukocyte marker Ptprc^a, commonly known as CD45.1 or Ly5.1. Wild-type C57BL and BALB/c inbred strains express the Ptprc^b (CD45.2 or Ly5.2) allele.

The current gene symbol designation Ptprc instead of Cd45 or Ly5 is based on work by Charbonneau and colleagues who showed in humans that a protein-tyrosine phosphatase (human placental protein-tyrosine phosphatase 1B) is homologous to the CD45 protein. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth.

A spontaneous mutation in the Ncr1 gene was reported to be segregating in this strain (Jang et al. 2018 J Immunol 200:1982). As of September 2020, our live colony no longer has this spontaneous mutation.

Chisolm et al. 2019 Immunity 51:155 characterized the SJL-derived genome surrounding the Ptprc^a allele on chromosome 1 using B6.SJL-CD45.1 mice obtained from Stock No. 002014. They identified a 37 Mb genomic interval of SJL genetic content including from Bcl2 to Glrx2 (Chr1:106,538,176-143,749,678; mm10 reference genome) - see Figure 4C (blue box) and Table S2 (the "Jax_B6.SJL-CD45.1 interval" tab). The publication describes how SJL genetic content in B6.SJL-CD45.1 mice may contribute to differences in amino acid content, H3K27Ac, gene regulation and protein expression in CD4+ Th1 cells. As such, it is recommended that researchers using Stock No. 002014 determine how/if any of the SJL genetic content may affect their specific experiments.

A CRISPR-generated coisogenic C57BL/6J CD45.1 strain which does not contain any additional SJL genetic contributions is now available as Stock No. 033076.

Development

The Ptprc^a allele and closely linked Pepc^b allele were serially backcrossed from SJL/J onto C57BL/6 with selection at each generation for Ptprc^a by serotyping (Shen et al., 1985). In 1990 this strain, then housed at Sloan Kettering, was imported into The Jackson Laboratory from Edward Boyse at generation N22. Since its arrival, the strain has been maintained by sibling inbreeding. In 2005 it reached generation N22F55.

A spontaneous mutation in the Ncr1 gene was reported to be segregating in this strain (Jang et al. 2018 J Immunol 200:1982). As of September 2020, our live colony no longer has this spontaneous mutation.

Chisolm et al. 2019 Immunity 51:155 characterized the SJL-derived genome surrounding the Ptprc^a allele on chromosome 1 using B6.SJL-CD45.1 mice obtained from Stock No. 002014. They identified a 37 Mb genomic interval of SJL genetic content including from Bcl2 to Glrx2 (Chr1:106,538,176-143,749,678; mm10 reference genome) - see Figure 4C (blue box) and Table S2 (the "Jax_B6.SJL-CD45.1 interval" tab). The publication describes how SJL genetic content in B6.SJL-CD45.1 mice may contribute to differences in amino acid content, H3K27Ac, gene regulation and protein expression in CD4+ Th1 cells. As such, it is recommended that researchers using Stock No. 002014 determine how/if any of the SJL genetic content may affect their specific experiments.

Allele

Symbol: Pepc^b
Name: b variant
MGI accession ID: MGI:4819861
Generation method: Not Applicable
Marker symbol: Pepc
Marker name: peptidase C
Chromosome: 1
Strain of origin: Not Applicable
Molecular note: The Pepc gene controls electrophoretic variation of peptidase C (PEP-C; EC 3.4.11.-) of red blood cells. The allele Pepc^b determines a faster migrating band and is found in the CBA/J and A/He strains.

Allele

Symbol: Ptprc^a
Name: a variant
MGI accession ID: MGI:4819849
Generation method: Not Applicable
Marker symbol: Ptprc
Marker name: protein tyrosine phosphatase, receptor type, C
Chromosome: 1
Strain of origin: Not Applicable
Site of expression: Widely expressed on all adaptive and innate immune cells.
Molecular note: Ptprc^a is found in strains SJL/J, STS/A, and DA. Ptprc^b is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485).

Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341).