The Tim mutation (translocation induced circling mutation) was induced by ethylene oxide in a mutagenesis experiment. The mutation was induced simultaneously with a reciprocal translocation between Chrs 4 and 17. Heterozygotes exhibit circling, head tossing, poor limb coordination, and corneal clouding. One mutated copy of the gene is dominant over two normal alleles.
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Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (other) | Tim | translocation induced circling mutation |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (other) | Ts(417)2Lws | trisomy 17, Chr 17 translocation to Chr4, Lewis 2 |
The Tim mutation (translocation induced circling mutation) was induced by ethylene oxide in a mutagenesis experiment. The mutation was induced simultaneously with a reciprocal translocation between Chrs 4 and 17. Because the mutation has so far been inseparable from the translocation, it may be that one of the translocation breakpoints has disrupted a gene on Chr 4 or 17. Heterozygotes exhibit circling, head tossing, poor limb coordination, and corneal clouding. The affected mice are usually smaller than wildtype littermates. Inner ear studies have not been done. The mutants swim poorly but can orient themselves in the water. One mutated copy of the gene is dominant over two normal alleles because segmentally trisomic mice with two normal Chrs 4 and 17 and the small 417 translocation product have the behavioral abnormalitites. Breeding is generally poor.
The original carrier mouse had a translocation between Chromosomes 4 and 17, which was also present in all but one mutant animal. The exceptional animal, which showed all mutant behavioral characteristics, had 41 chromosomes which included two normal 4 and 17 homologs and the small 4(17) translocation chromosome.
Allele Name | nonagouti |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 2 |
General Note | Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039 |
Molecular Note | Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type. |
Allele Name | wild-type agouti |
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Allele Type | Spontaneous |
Allele Synonym(s) | dark-bellied agouti |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | various |
Chromosome | 2 |
General Note | The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back. |
Molecular Note | This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene. |
Allele Name | translocation induced circling mutation 3 Lewis |
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Allele Type | Chemically induced (other) |
Allele Synonym(s) | Tim |
Gene Symbol and Name | Tim, translocation induced circling mutation |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6J x DBA/2J)F1 |
Chromosome | 4 |
Molecular Note | The translocation breakpoint in Chr4 produced this allele. |
Allele Name | trisomy 17, Chr 17 translocation to Chr 4, Lewis 2 |
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Allele Type | Chemically induced (other) |
Allele Synonym(s) | T2Lws |
Gene Symbol and Name | Ts(417)2Lws, trisomy 17, Chr 17 translocation to Chr4, Lewis 2 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6J x DBA/2J)F1 |
Chromosome | 17 |
Molecular Note | Breakpoints for chromosome 17 span from E4 to the telomere where induced by treatment with ethylene oxide. This sequence was fused to chromosome 4, demonstrating breakpoints spanning from the centromere to 4A4. |
When using the B6EiC3Sn a/A-Ts(417)2Lws TimT(4;17)3Lws/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001923 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozyous or wildtype for Ts(4<17>)2Lws Tim<T(4;17)3Lws> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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