These mice carry a spontaneous mutation at the Cacng2 locus characterized by whole body tremor, an unstable gait, growth retardation, and occasional hydrocephaly.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Cacng2 | calcium channel, voltage-dependent, gamma subunit 2 |
In the waggler spontaneous mutation, an allelic variant of stargazer, the expression of brain-derived neurotrophic factor is selectively absent in the cerebellar granule cells. The cytoarchitecture of the waggler cerebellum appears to be normal at the light microscope level. The mutant mice exhibit no sensory deficits to auditory stimuli or heat-induced pain. However, they are massively impaired in classic eye-blink conditioning. The waggler allele causes a great variation in spike-wave discharge activity depending on the individual mouse.
The waggler mutation arose spontaneously on the MRL/MpJ-Tnfrsf6lpr background in the laboratory of Dr. Charles Sidman in 1988. This mutation was backcrossed to C57BL/6J via backcross-intercross and the lpr mutation was removed from the strain. Ovary transplantation from homozygous females was used when it became necessary. In 1991 N6 heterozygotes were bred together to generate embryos for cryopreservation.
Allele Name | waggler |
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Allele Type | Spontaneous |
Allele Synonym(s) | wag |
Gene Symbol and Name | Cacng2, calcium channel, voltage-dependent, gamma subunit 2 |
Gene Synonym(s) | |
Strain of Origin | MRL/MpJ-Faslpr/J |
Chromosome | 15 |
General Note | Waggler is a second recessive mutation at the Cacng2 locus that arose in 1988 in the MRL/MpJ-Tnfrsf6 colony maintained at The Jackson Laboratory by Dr. Charles Sidman. Mutant homozygotes are characterized by whole body tremor, an unstable gait, and growth retardation. Hydrocephaly occurs occasionally. Hearing tests by Dr. Lawrence Erway of the University of Cinncinnati showed that the mutant was not deaf. Both sexes breed; no gross neuroanatomical or histopathologic lesions have been found (J:14313). |
Molecular Note | The phenotype of the waggler is associated with aberrant splicing residing in the first intron. Like the stargazer allele, a RT-PCR analysis demonstrated that in addition to aberrant transcripts, some normally spliced mRNA was detected, suggesting that this mutation does not represent a complete null allele. |
When maintaining a live colony, these mice can be bred as homozygous females to heterozygous males. In our colony, homozygous males did not breed.
When using the waggler mouse strain in a publication, please cite the originating article(s) and include JAX stock #001883 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Homozygous for stg<wag> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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