Overview

This substrain of the recombinant inbred strain MEV/1Ty carries the MuLV endogenous ecotropic gene Emv34.

Genetic overview

Genetic Background	Generation

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Research Applications

Mouse/Human Gene Homologs
Dermatology Research
Research Tools
Virology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

In 1989 Taylor and Rowe described generating a linkage testing stock homozygous for 11 ecotropic MuLV proviruses on 10 distinct chromosomes. The strain was named MEV/1Ty for Multiple Ecotropic proVirus. These proviral insertions can be identified via Southern blot analysis of PvuII or HindIII digests using the MuLV-specific pEcB4 probe. The first progenitor strain C58/J contributed the following proviruses identified by the PvuII junction fragments indicated in parenthesis: Emv20 (8.2kb), Emv21 (6.6 kb), Emv23 (3.7 kb), Emv24 (3.5 kb), Emv25 (3.4 kb), Emv26 (2.9 kb), and Emv27 (9.4 kb). The second progenitor strain AKXD-14/Ty contributed Emv11 (4.3 kb), Emv13 (3.4 kb), and Emv14 (8.6 kb) from AKR/J and Emv3 (5.2 kb) from DBA/2J. Emv3 is the proviral insertion causing the dilute allele of Myo5a. This strain is also homozygous for nonagouti (a). Further analysis of MEV/1Ty progeny identified subsequent MuLV provirus re-insertions that were used to generate subsequent strains with more proviral mapping markers. This strain, MEV-Q/Ty carries Emv34 (Taylor et al., 1989, 1991, and 1993.)

Development

MEV/1Ty was developed by crossing C58/J with AKXD-14/Ty, and generating multiple recombinant inbred lines. At F6 these lines were tested and one that was found to carry all of the multiple ecotropic MuLV proviruses of interest was expanded. At F9 one line was found to be homozygous for all but 3 of the multiple ecotropic MuLV proviruses of interest. This line was further inbred and at F12 was homozygous for the multiple ecotropic MuLV proviruses of interest along with the nonagouti and dilute coat color alleles. Proviral re-insertions were identified and characterized on MEV/1Ty and enhanced substrains of MEV/1Ty were developed that carry these proviruses. MEV-Q/Ty carries Emv34 (Taylor and Rowe, 1989, 1991, 1993.)

Genetics

Myo5a^d

Allele Symbol: Myo5a^d

Allele Name	dilute
Allele Type	Spontaneous
Allele Synonym(s)	blue dilution; d; d^v; Maltese dilution
Gene Symbol and Name	Myo5a, myosin VA
Gene Synonym(s)
Strain of Origin	old mutant of the mouse fancy
Chromosome	9
Molecular Note	This mutation is the result of the integration of ecotropic murine leukemia virus Emv-3 into a noncoding region of the Myo5a^d gene. Reversions of Myo5a^d to wild-type are caused by excision of the virus leaving exactly one long terminal repeat in place.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Myo5a^d related

Mouse/Human Gene Homologs
- Griscelli Syndrome
Dermatology Research
- Color and White Spotting Defects

Dermatology Research
- Color and White Spotting Defects
Research Tools
- Genetics Research
  - Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis
  - Gene Mapping
Virology Research

Mammalian Phenotype Terms by Genotype

References

Additional References

Taylor BA; Rowe L. 1989. A mouse linkage testing stock possessing multiple copies of the endogenous ecotropic murine leukemia virus genome. Genomics 5(2):221-32PubMed: 2571572 MGI: J:10034
Taylor BA; Rowe L; Grieco DA. 1993. The MEV mouse linkage testing stock: mapping 30 novel proviral insertions and establishment of an improved stock. Genomics 16(2):380-94PubMed: 8390965 MGI: J:4958

Additional - Myo5a^d related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the MEV-Q/TyJ mouse strain in a publication, please include JAX stock #001850 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Inbred, 1 pair minimum will be supplied

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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