A/J-Abcg5^trac/J

Stock number: 001830
Product name: thrombocytopenia and cardiomyopathy
Research areas: Cardiovascular Research, Hematological Research

Description

These mice carry a spontaneous mutation at the Abcg5 locus characterized by macrothrombocytopenia, prolonged bleeding times, anemia, leukopenia, infertility, cardiomyopathy, and shortened life span.

Detailed description

The spontaneous mouse mutation 'thrombocytopenia and cardiomyopathy (Abcg5^trac) causes macrothrombocytopenia, prolonged bleeding times, anemia, leukopenia, infertility, cardiomyopathy, and shortened life span. Homozygotes show a 20-fold decrease in platelet numbers and a 3-fold increase in platelet size with structural alterations and functional impairments in activation and aggregation. Megakaryocytes in homozygous mice are present in increased numbers, have poorly developed demarcation membrane systems, and have decreased polyploidy. The thrombocytopenia is not intrinsic to defects at the level of hematopoietic progenitor cells but is associated with a microenvironmental abnormality. The Abcg5^trac mutation maps to mouse chromosome 17, syntenic with human chromosome 2p21-22. A G to A mutation in exon 10 of the adenosine triphosphate (ATP)-binding cassette subfamily G, member 5 gene, alters a tryptophan codon (UGG) to a premature stop codon (UAG). Crosses with mice doubly transgenic for the human ABCG5 and ABCG8 genes rescued platelet counts and volumes. ABCG5 and ABCG8 form a functional complex that limits dietary phytosterol accumulation. Phytosterolemia in homozygous mice confirmed a functional defect in the ABCG5/ABCG8 transport system. The Abcg5^trac mutation provides a new clinically significant animal model for human phytosterolemia and provides a new means for studying the role of phytosterols in hematologic diseases and testing therapeutic interventions.

Development

The thrombocytopenia and cardiomyopathy (trac) mutation arose spontaneously on the A/J inbred strain at The Jackson Laboratory in 1989 and was initially called cardiomyopathy, cmp. It was maintained by closed colony inbreeding, primarily sibling inbreeding, and in 1991 embryos were cryopreserved from +/? females bred with heterozygous males all between generation F7 and F11.

Allele

Symbol: Abcg5^trac
Name: thrombocytopenia and cardiomyopathy
MGI accession ID: MGI:2156990
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Abcg5
Marker name: ATP binding cassette subfamily G member 5
Chromosome: 17
Strain of origin: A/J
Molecular note: Sequencing of the cDNA revealed a G-to-A mutation in exon 10 of the gene, which alters a tryptophan codon 462 (UGG) to a premature stop codon (UAG) (p.W462*). The premature stop codon is predicted to result the expression of a mutant protein that lacks the last 4 transmembrane domains.