These mice carry a spontaneous mutation at the Abcg5 locus characterized by macrothrombocytopenia, prolonged bleeding times, anemia, leukopenia, infertility, cardiomyopathy, and shortened life span.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Null/Knockout) | Abcg5 | ATP binding cassette subfamily G member 5 |
The spontaneous mouse mutation 'thrombocytopenia and cardiomyopathy (Abcg5trac) causes macrothrombocytopenia, prolonged bleeding times, anemia, leukopenia, infertility, cardiomyopathy, and shortened life span. Homozygotes show a 20-fold decrease in platelet numbers and a 3-fold increase in platelet size with structural alterations and functional impairments in activation and aggregation. Megakaryocytes in homozygous mice are present in increased numbers, have poorly developed demarcation membrane systems, and have decreased polyploidy. The thrombocytopenia is not intrinsic to defects at the level of hematopoietic progenitor cells but is associated with a microenvironmental abnormality. The Abcg5trac mutation maps to mouse chromosome 17, syntenic with human chromosome 2p21-22. A G to A mutation in exon 10 of the adenosine triphosphate (ATP)-binding cassette subfamily G, member 5 gene, alters a tryptophan codon (UGG) to a premature stop codon (UAG). Crosses with mice doubly transgenic for the human ABCG5 and ABCG8 genes rescued platelet counts and volumes. ABCG5 and ABCG8 form a functional complex that limits dietary phytosterol accumulation. Phytosterolemia in homozygous mice confirmed a functional defect in the ABCG5/ABCG8 transport system. The Abcg5trac mutation provides a new clinically significant animal model for human phytosterolemia and provides a new means for studying the role of phytosterols in hematologic diseases and testing therapeutic interventions.
The thrombocytopenia and cardiomyopathy (trac) mutation arose spontaneously on the A/J inbred strain at The Jackson Laboratory in 1989 and was initially called cardiomyopathy, cmp. It was maintained by closed colony inbreeding, primarily sibling inbreeding, and in 1991 embryos were cryopreserved from +/? females bred with heterozygous males all between generation F7 and F11.
Allele Name | thrombocytopenia and cardiomyopathy |
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Allele Type | Spontaneous (Null/Knockout) |
Allele Synonym(s) | cardiomyopathy; cmp; tac |
Gene Symbol and Name | Abcg5, ATP binding cassette subfamily G member 5 |
Gene Synonym(s) | |
Strain of Origin | A/J |
Chromosome | 17 |
Molecular Note | Sequencing of the cDNA revealed a G-to-A mutation in exon 10 of the gene, which alters a tryptophan codon 462 (UGG) to a premature stop codon (UAG) (p.W462*). The premature stop codon is predicted to result the expression of a mutant protein that lacks the last 4 transmembrane domains. |
When using the thrombocytopenia and cardiomyopathy mouse strain in a publication, please cite the originating article(s) and include JAX stock #001830 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for trac |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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