Hybridomas are produced by fusing mouse meloma cells that are deficient in adenosine phosphoribosyltransferase (APRT) with spleen cells from mice immunized with specific antigens. A selection system using AAT (adenosine, aminopterin, and thymidine) medium eliminates all but the fusion cells that are APRT+ . Successful hybridomas must also retain the Igh heavy chain locus on chromosome 12 and one of the Igh light chain loci. The Robertsonian chromosome Rb(8.12)5Bnr can be used to produce hybridomas at a high frequency. Selecting for the Aprt locus on chromosome 8 also selects for the Igh locus on chromosome 12 because the two loci are "genetically linked" by the Robertsonian fusion chromosome. This chromosomal aberration congenic was created by backcrossing the Robertsonian chromosome Rb(8.12)5Bnr on the BALB/cByJ genetic background by Dr. Muriel Davisson at The Jackson Laboratory.
When using the CByJ.RBF-Rb(8.12)5Bnr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001802 in your Materials and Methods section.