Mice of this strain carry a Robertsonian chromosome composed of chromosomes 8 and 12 and can be used to produce hybridomas at a high frequency.
Read More +Genetic Background | Generation |
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001026 BALB/cByJ |
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Marker Symbol | Marker Name | |
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Rb(8.12)5Bnr | Robertsonian translocation, Chr 8 and 12, Universitat Bonn/Rhein 5 |
Hybridomas are produced by fusing mouse meloma cells that are deficient in adenosine phosphoribosyltransferase (APRT) with spleen cells from mice immunized with specific antigens. A selection system using AAT (adenosine, aminopterin, and thymidine) medium eliminates all but the fusion cells that are APRT+ . Successful hybridomas must also retain the Igh heavy chain locus on chromosome 12 and one of the Igh light chain loci. The Robertsonian chromosome Rb(8.12)5Bnr can be used to produce hybridomas at a high frequency. Selecting for the Aprt locus on chromosome 8 also selects for the Igh locus on chromosome 12 because the two loci are "genetically linked" by the Robertsonian fusion chromosome. This chromosomal aberration congenic was created by backcrossing the Robertsonian chromosome Rb(8.12)5Bnr on the BALB/cByJ genetic background by Dr. Muriel Davisson at The Jackson Laboratory.
Marker Synonym(s) | |
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Chromosome(s) | 12 |
When using the CByJ.RBF-Rb(8.12)5Bnr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001802 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Rb(8.12)5Bnr, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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