Genotyping Protocols

• QPCR:Hya
• QPCR:Sry
• Separated PCR:A A A Alternate2
• Sanger sequencing:Eda-SEQ
• Genotyping resources and troubleshooting

Breeding Considerations

Comments: This strain is maintained using Eda^Ta-6J as a phenotypic marker to expedite progeny testing at each of the two alternate generations. In the first generation a X^Eda-Ta-6JY^? male is bred to a C57BL/6J-A^w-J female (Stock No. 000051); striped male offspring (genotype XX^{Eda-Ta-6J sxrb}) prove that the male parent is X^Eda-Ta-6JY^sxrb. Four genotypically distinct offspring result from the breeding scheme C57BL/6J-A^w-J x X^Eda-Ta-6JY^sxrb: XX^Eda-Ta-6J transversely striped females; XY normal males; XY^sxrb phenotypically normal males; and XX^{Eda-Ta-6J sxrb} which are transversely striped, sterile males due to sex-reversion and which prove the paternal genotype. The phenotypically normal males are then bred with heterozygous C57BL/6J-A^w-J-Eda^Ta-6J females (Stock No. 000338) in the second generation of breeding. Again, transversely striped male offspring (genotype XX^{Ta-6J sxrb}) of these breeding pairs prove that the male parent is a carrier of Y^sxrb. Eight genotypically distinct offspring result from the breeding scheme heterozygous C57BL/6J-A^w-J-Eda^Ta-6J x XY^sxrb: XY normal males; XY^sxrb phenotypically normal males; X^Eda-Ta-6JY tabby males; X^Eda-Ta-6JY^sxrb tabby males; XX normal females; X^Eda-Ta-6JX transversely striped females; XX^sxrb sterile males; and X^Eda-Ta-6JX^sxrb transversely striped sterile males that prove the paternal genotype to be XY^sxrb. The Tabby male progeny (X^Eda-Ta-6JY^?) are then bred with C57BL/6J-A^w-J females for the first generation progeny test (C57BL/6J-A^w-J x X^Eda-Ta-6JY^sxrb). XX^sxrb sex-reversed males can be distinguished from XY and XY^sxrb males by their small testes which are detectable by palpation at 6-8 weeks of age. C57BL/6J-A^w-J is used in breeding because the agouti coat color is necessary for viewing the Eda^Ta-6J phenotypes. It is important to note that only the X^sxrb carrying progeny of these breeding schemes will be sex reversed; XY^sxrb mice a re male, not sex reversed. XX^sxrb and X^Eda-Ta-6JX^sxrb sex-reversed males are sterile.

• Additional Breeding and Husbandry Support

Citation

When using the B6.Cg-A^w-J Eda^Ta-6J Sxr^b Hya^-/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001730 in your Materials and Methods section.