Genetic Background | Generation |
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000664 C57BL/6J |
|
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Eda | ectodysplasin-A |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Tp(Y)1Ct | transposition, Chr Y, Cattanach 1 |
Marker Symbol | Marker Name | |
---|---|---|
Hya | histocompatibility Y |
Allele Name | tabby 6 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | Ta6J; Ta2; Tabby |
Gene Symbol and Name | Eda, ectodysplasin-A |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J-tp3J/J |
Chromosome | X |
Molecular Note | This allele is defined by a base pair deletion (at position 1049) resulting in a frameshift mutation. |
Allele Name | Sex reversed, b variant |
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Allele Type | Spontaneous |
Allele Synonym(s) | HY-; Sxr' |
Gene Symbol and Name | Tp(Y)1Ct, transposition, Chr Y, Cattanach 1 |
Gene Synonym(s) | |
Strain of Origin | T16H stock |
Chromosome | Y |
Molecular Note | At least five loci map to the Tp(Y)1Ct segment: Hya, Spy, Tdy, Zfy1, and Zfy2. The Tp(Y)1CtSxr-b variant retained the testis determining gene, Sry, but lost the H-Y antigen-determining gene, Hya. This chromosomal variant also appears to lack one of the Y-linked zinc finger genes, Zfy1 but retains the other, Zfy2. The formation of Tp(Y)1CtSxr-b from Tp(Y)1CtSxr-a was later shown to be due to unequal crossing over between Zfy1 and Zfy2, resulting in a fusion gene and a small interstitial deletion. XX females carrying this transposition variant are sex-reversed but do not express Y Chromosome linked transplantation antigen, Hya. |
Marker Synonym(s) | |
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Chromosome(s) | Y |
Comments: This strain is maintained using EdaTa-6J as a phenotypic marker to expedite progeny testing at each of the two alternate generations. In the first generation a XEda-Ta-6JY? male is bred to a C57BL/6J-Aw-J female (Stock No. 000051); striped male offspring (genotype XXEda-Ta-6J sxrb) prove that the male parent is XEda-Ta-6JYsxrb. Four genotypically distinct offspring result from the breeding scheme C57BL/6J-Aw-J x XEda-Ta-6JYsxrb: XXEda-Ta-6J transversely striped females; XY normal males; XYsxrb phenotypically normal males; and XXEda-Ta-6J sxrb which are transversely striped, sterile males due to sex-reversion and which prove the paternal genotype. The phenotypically normal males are then bred with heterozygous C57BL/6J-Aw-J-EdaTa-6J females (Stock No. 000338) in the second generation of breeding. Again, transversely striped male offspring (genotype XXTa-6J sxrb) of these breeding pairs prove that the male parent is a carrier of Ysxrb. Eight genotypically distinct offspring result from the breeding scheme heterozygous C57BL/6J-Aw-J-EdaTa-6J x XYsxrb: XY normal males; XYsxrb phenotypically normal males; XEda-Ta-6JY tabby males; XEda-Ta-6JYsxrb tabby males; XX normal females; XEda-Ta-6JX transversely striped females; XXsxrb sterile males; and XEda-Ta-6JXsxrb transversely striped sterile males that prove the paternal genotype to be XYsxrb. The Tabby male progeny (XEda-Ta-6JY?) are then bred with C57BL/6J-Aw-J females for the first generation progeny test (C57BL/6J-Aw-J x XEda-Ta-6JYsxrb). XXsxrb sex-reversed males can be distinguished from XY and XYsxrb males by their small testes which are detectable by palpation at 6-8 weeks of age. C57BL/6J-Aw-J is used in breeding because the agouti coat color is necessary for viewing the EdaTa-6J phenotypes. It is important to note that only the Xsxrb carrying progeny of these breeding schemes will be sex reversed; XYsxrb mice a re male, not sex reversed. XXsxrb and XEda-Ta-6JXsxrb sex-reversed males are sterile.
When using the B6.Cg-Aw-J EdaTa-6J Sxrb Hya-/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001730 in your Materials and Methods section.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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