Overview

Also Known As:Lethal 3

This multigenic deletion in Chromosome 1 causes embryonic lethality in homozygotes.

Genetic overview

Genetic Background	Generation

l1Rk3

Allele Type	Gene Symbol	Gene Name
Chemically induced (other)	l1Rk3	lethal, Chr 1, Roderick 3

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Research Applications

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This chemically-induced multigenic deletion in Chromosome 1, which includes Mlph in the deletion, causes embryonic lethality in homozygotes. Compound heterozgyotes of Mlph^ln/l1Rk3 are viable but have the diluted coat color caused by Mlph mutations.

Development

The In(1)1Rk inversion was radiation-induced in a DBA/2J male and this male was immediately bred to a C57BL/6J female. Many years later, a male homozygous for In(1)1Rk was exposed to 0.3 mg/kg triethylene melamine and mated to females heterozygous for splotch and homozygous for leaden (Pax3^Sp Mlph^ln/Pax3⁺ Mlph^ln) on a predominantly C57BL/6J background in order to screen for radiation-induced lethal mutations in the In(1)1Rk-bearing chromosome. The offspring with the splotch phenotype, which were necessarily In(1)1Rk + +/+ Pax3^Sp Mlph^ln, were intercrossed generating live offspring that all had the splotch phenotype (In(1)1Rk + +/+ Pax3^Sp Mlph^ln). The absence of Pax3⁺ pups proved the presence of a recessive lethal mutation on the In(1)1Rk-bearing chromosome, which was designated l1Rk3. The repulsion heterozygotes (l1Rk3 + +/+ Pax3^Sp Mlph^ln), identified by the phenotypic presence of splotch and absence of the recessive leaden phenotype, were intercrossed at each generation to maintain this strain and embryos were generated for cryopreservation from repulsion heterozygotes. The cryo-recovered pups that have the splotch phenotype but not the leaden phenotype are the repulsion heterozygotes that should be used to maintain this line.

Selected References

Matesic LE; Yip R; Reuss AE; Swing DA; O'Sullivan TN; Fletcher CF; Copeland NG; Jenkins NA. 2001. Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice. Proc Natl Acad Sci U S A 98(18):10238-43PubMed: 11504925 MGI: J:71302

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Genetics

l1Rk3

Allele Symbol: l1Rk3

Allele Name	lethal, Chr 1, Roderick 3
Allele Type	Chemically induced (other)
Allele Synonym(s)
Gene Symbol and Name	l1Rk3, lethal, Chr 1, Roderick 3
Gene Synonym(s)
Strain of Origin	Not Specified
Chromosome	1
General Note	This homozygous recessive lethal mutation was induced using triethylene melamine at 0.3 mg/kg.
Molecular Note	Sequence analysis of cDNA showed this allele comprises, minimally, a deletion of the coding region of Mlph, resulting in no expression of Mlph transcripts.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: l1Rk3/l1Rk3
involves: C57L/J

mortality/aging

prenatal lethality

The following phenotype relates to a compound genotype created using this strain

Genotype: l1Rk3/Mlph^ln
involves: C57L/J

integument phenotype

diluted coat color
- phenotype was described as being similar to the original leaden allele

pigmentation phenotype

diluted coat color
- phenotype was described as being similar to the original leaden allele

References

Matesic LE; Yip R; Reuss AE; Swing DA; O'Sullivan TN; Fletcher CF; Copeland NG; Jenkins NA. 2001. Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice. Proc Natl Acad Sci U S A 98(18):10238-43PubMed: 11504925 MGI: J:71302

Additional - l1Rk3 related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the Lethal 3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #001640 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	l1Rk3 in repulsion with splotch and leaden

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

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Licensing Information

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Also Known As:Lethal 3

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

l1Rk3

Allele Symbol: l1Rk3

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: l1Rk3/l1Rk3involves: C57L/J

mortality/aging

Genotype: l1Rk3/Mlphlninvolves: C57L/J

integument phenotype

pigmentation phenotype

References

Additional - l1Rk3 related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Genotype: l1Rk3/l1Rk3
involves: C57L/J

Genotype: l1Rk3/Mlph^ln
involves: C57L/J