STOCK Mlph^ln-l1Rk3/J

Stock number: 001640
Product name: Lethal 3

Description

This multigenic deletion in Chromosome 1 causes embryonic lethality in homozygotes.

Detailed description

This chemically-induced multigenic deletion in Chromosome 1, which includes Mlph in the deletion, causes embryonic lethality in homozygotes. Compound heterozgyotes of Mlph^ln/l1Rk3 are viable but have the diluted coat color caused by Mlph mutations.

Development

The In(1)1Rk inversion was radiation-induced in a DBA/2J male and this male was immediately bred to a C57BL/6J female. Many years later, a male homozygous for In(1)1Rk was exposed to 0.3 mg/kg triethylene melamine and mated to females heterozygous for splotch and homozygous for leaden (Pax3^Sp Mlph^ln/Pax3⁺ Mlph^ln) on a predominantly C57BL/6J background in order to screen for radiation-induced lethal mutations in the In(1)1Rk-bearing chromosome. The offspring with the splotch phenotype, which were necessarily In(1)1Rk + +/+ Pax3^Sp Mlph^ln, were intercrossed generating live offspring that all had the splotch phenotype (In(1)1Rk + +/+ Pax3^Sp Mlph^ln). The absence of Pax3⁺ pups proved the presence of a recessive lethal mutation on the In(1)1Rk-bearing chromosome, which was designated l1Rk3. The repulsion heterozygotes (l1Rk3 + +/+ Pax3^Sp Mlph^ln), identified by the phenotypic presence of splotch and absence of the recessive leaden phenotype, were intercrossed at each generation to maintain this strain and embryos were generated for cryopreservation from repulsion heterozygotes. The cryo-recovered pups that have the splotch phenotype but not the leaden phenotype are the repulsion heterozygotes that should be used to maintain this line.

Allele

Symbol: l1Rk3
Name: lethal, Chr 1, Roderick 3
MGI accession ID: MGI:1933755
Generation method: Chemically induced (other)
Marker symbol: l1Rk3
Marker name: lethal, Chr 1, Roderick 3
Chromosome: 1
Strain of origin: Not Specified
Molecular note: Sequence analysis of cDNA showed this allele comprises, minimally, a deletion of the coding region of Mlph, resulting in no expression of Mlph transcripts.
General note: This homozygous recessive lethal mutation was induced using triethylene melamine at 0.3 mg/kg.