These mice carry an ENU-induced mutation characterized by renal tubular acidosis, doubled urine output, increased urine pH and Cl-, decreased blood pH and HCO-3, phosphatemia, lower plasma bicarbonate, and a smaller body size.
Read More +Genetic Background | Generation |
---|---|
000664 C57BL/6J |
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) | Car2 | carbonic anhydrase 2 |
In human, CAR2 deficiencies can result in osteopetrosis, renal tubular acidosis, impaired growth, cerebral calcification, and mental retardation. Mice homozygous for Car2<n> do not display cerebral calcification or osteopetrosis, although medial calcification of small arteries has been found in several organs, most notably the male genital tract. The phenotype of these mice includes renal tubular acidosis, doubled urine output, increased urine pH and Cl-, decreased blood pH and HCO-3, phosphatemia, lower plasma bicarbonate, and a smaller body size. Despite smaller body weight, the kidneys are of normal size and plasma potassium and creatinine levels are normal. Type A and B intercalated cells of the kidney are severely depleted. Car2n homozygotes have reduced susceptibility to audiogenic and chemogenic seizures and hippocampal slices from homozygotes are more resistant to hypoxia than are those of wild type controls. Increased CAR4 levels, suggesting a compensation mechanism, have been found in oligodendrocytes and myelinated tracts. Successful liposome-delivered gene therapy has been performed on this mutant. (Lewis et al., 1988; Spicer et al., 1989; Brechue et al., 1991; Tashian, 1992; Velisek et al., 1993A and B, and 1995; Brion et al., 1994 and 1997; Breton et al.1995; Lai et al, 1998.)
DBA/2J males were treated with 200mg/kg N-ethyl-N-nitrosourea then mated to C57BL/6J females10 or more weeks after treatment. Blood and kidney samples from the offspring were assayed by isoelectric focusing and a male was identified that lacked the DBA/2J derived Car2b band. The C57BL/6J derived Car2a band was detected. This mutant male was backcrossed to C57BL/6J. Approximately one quarter of offspring from the mating of these F1 to their mutant father were found to have no Car2 bands in any of multiple tissues assayed. In 1988 this mutation was imported into the laboratory of Dr. Jane Barker at The Jackson Laboratory from Dr. Susan Lewis at Research Triangle Institute. Dr. Barker backcrossed it further onto C57BL/6J and in 1995 C57BL/6J females were bred with homozygous B6.D2-Car2n males, then at N19, and the resulting embryos were cryopreserved. (Lewis et al., 1988; Johnson and Lewis, 1981.)
Allele Name | null |
---|---|
Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | CA(II)D; CAII-; Car2-; Car-20; Car2N |
Gene Symbol and Name | Car2, carbonic anhydrase 2 |
Gene Synonym(s) | |
Strain of Origin | DBA/2J |
Chromosome | 3 |
Molecular Note | The result of ENU mutagenesis, the mutation at the Car2 locus is likely a nonsense or missense mutation. Molecular analysis shows that the mutant gene is not deleted but is transcribed. The CAR2 protein could not be detected by immunodiffusion analysis and Southern analysis indicates no detectable rearrangements or polymorphisms. |
Car2n homozygotes are intolerant of NH4Cl in their drinking water. Homozygous females are poor breeders, often producing stillborn pups. Male homozygotes can be used for breeding.
When using the CA II-deficient mouse strain in a publication, please cite the originating article(s) and include JAX stock #001623 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Car2<n> |
Frozen Mouse Embryo | B6.D2-Car2<n>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.D2-Car2<n>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.D2-Car2<n>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.D2-Car2<n>/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.