B6.D2-Kit^W-73J/J

Stock number: 001563
Product name: dominant spotting 73 Jackson
Strain synonyms: W⁷³
Research areas: Cancer Research, Dermatology Research, Developmental Biology Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Mouse/Human Gene Homologs, Neurobiology Research, Reproductive Biology Research, Sensorineural Research

Detailed description

These mice carry a spontaneous remutation at the Kit locus. Kit^W-73J interacts with both Kit^W-v/+ and Mitf^Mi-wh/+ to produce black eyed white offspring in the F1. Homozygous Kit^W-73J mice are not viable.

Development

The Kit^W-73J remutation occurred in 1998 on DBA/2J (Stock No. 000671), and was cryopreserved in 1989 by mating heterozygous males to C57BL/6J females.

Allele

Symbol: Kit^W-73J
Name: dominant spotting 73 Jackson
MGI accession ID: MGI:1856255
Generation method: Spontaneous
Marker symbol: Kit
Marker name: KIT proto-oncogene receptor tyrosine kinase
Chromosome: 5
Strain of origin: DBA/2J
Molecular note: Direct sequence of this allele and comparison with normal c-kit indicated a nonsynonymous coding mutation of a C-to-A point mutation in ATP biding loop consensus sequence of the kinase domain that result in replacement of the alanine with an aspartate residue at amino acid 605 position (p.A605D).