This spontaneous mutation causes enlarged sebaceous glands with dense, irregularly-shaped lipid droplets, mild hyperkeratosis, unkempt greasy fur, and in females decreased abdominal adipose tissue.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Mpzl3 | myelin protein zero-like 3 |
Rough fur homozygotes can be identified by the unkempt and greasy appearance of the fur at wean age and this persists throughout life. Palanza et al. reported that alopecia progresses such that by 28 weeks of age more than 80% of homozygotes have large bald patches affecting between 5 and 90% of the skin. They have mild hyperkeratosis of the skin and broken hairs. Park et al found hyper-sebum production with enlarged sebaceous glands having lipid droplets that are denser than normal and irregularly shaped. However, the hair follicles are normally formed and evenly distributed. Palanza et al. also reported that homozygous females have a decrease in abdominal adipose tissue at 28 weeks of age that is threefold lower than that of controls.
The rough fur mutation arose spontaneously in 1985 in the inbred strain C3H/HeJ and was maintained coisogenic on this background by sibling breeding. Embryos were generated for cryopreservation from C3HeB/FeJ females bred to homozygous males at generation F15 and F16. Many years later two female heterozygotes thawed from this bankstock were bred to C3H/HeJ and their male offspring were backcrossed to the heterozygous mothers to generate homozygous males that were used for cryopreservation of sperm.
Allele Name | rough fur |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Mpzl3, myelin protein zero-like 3 |
Gene Synonym(s) | |
Strain of Origin | C3H/HeJ |
Chromosome | 9 |
Molecular Note | Complimentation assay with Mpzl3rc determined gene identity. However, sequence analysis failed to identify mutations in the promoter, exons and splice junctions. |
When using the rough fur mouse strain in a publication, please cite the originating article(s) and include JAX stock #001544 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for ruf |
Frozen Mouse Embryo | C3Fe;C3-Mpzl3<ruf>/J | $2595.00 |
Frozen Mouse Embryo | C3Fe;C3-Mpzl3<ruf>/J | $2595.00 |
Frozen Mouse Embryo | C3Fe;C3-Mpzl3<ruf>/J | $3373.50 |
Frozen Mouse Embryo | C3Fe;C3-Mpzl3<ruf>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.