Both homozygous and heterozygous mice with the contrasted induced mutation (KitlSl-con) are viable. Male homozygous mutant mice are fertile but females are usually sterile. Mice heterozygous for the contrasted mutation are recognizable soon after birth by dark pigmentation of the genital papilla with the adult coat being slightly lighter than normal. Homozygous mutant mice also have dark genitalia and a markedly diluted coat and mild macrocytic anemia.
|Allele Name||steel contrasted|
|Allele Type||Radiation induced|
|Gene Symbol and Name||Kitl, kit ligand|
|Strain of Origin||(C3H/HeH x 101/H)F1|
|General Note||The contrasted mutation was found in a neutron irradiation experiment. Heterozygotes are recognizable soon after birth by dark pigmentation of the genital papilla. The adult coat is slightly lighter than normal. Homozygotes also have dark external genitalia but a markedly diluted coat; they have a mild macrocytic anemia. Males are fertile but females are usually sterile (J:20215).|
|Molecular Note||Pulse field gel electrophoresis using cDNA and genomic clones showed that this allele is an undefined rearrangement between 90 and 2.2Mb 5' to Kitl. Northern analysis showed that the mRNA transcript is of normal size but this allele exerts tissue-specific effects on transcript abundance.|