The Uoxin homozygous phenotype has incomplete penetrance. While 63% of Uoxin homozygotes die by 12-14 days of age, those that live to adulthood generally live a normal breeding life span. Homozygous adults display chronic polyuria, increased serum BUN and creatinine levels, and hydronephrosis with a concomitant inflammatory response that is followed by glomerular and tubular dilation. (Cook et al., 2001.)
This paracentric inversion was identified in a male offspring of a C57BL/6J female bred to a DBA/2J male that had received 1000 R cesium exposure. That male was bred to a C57BL/6J female and the strain was eventually maintained by sibling intercrossing homozygotes. Embryos were generated for cryopreservation by crossing homozygous males with C57BL/6J females.
|Allele Name||urate oxidase In(3)55Rk-induced|
|Allele Type||Radiation induced|
|Gene Symbol and Name||Uox, urate oxidase|
|Strain of Origin||DBA/2J|
|General Note||See also In(3)55Rk-d and In(3)55Rk-p.|
|Molecular Note||PCR analysis on genomic DNA derived from mice carrying the In(3)55K inversion suggests that a disruption 3' to base 844 of exon 7 results in the mutation. Northern blot analysis on mRNA derived from liver RNA of homozygous mice confirmed that negligible amounts of encoded transcript was produced from this allele.|
When using the B6;D2-In(3)55Rk Uoxin/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001378 in your Materials and Methods section.