B6;D2-In(3)55Rk Uoxⁱⁿ/J

Stock number: 001378
Research areas: Developmental Biology Research, Internal/Organ Research

Description

These mice carry a cesium irradiation -induced mutation characterized by chronic polyuria, increased serum BUN and creatinine levels, and hydronephrosis.

Detailed description

The Uoxⁱⁿ homozygous phenotype has incomplete penetrance. While 63% of Uoxⁱⁿ homozygotes die by 12-14 days of age, those that live to adulthood generally live a normal breeding life span. Homozygous adults display chronic polyuria, increased serum BUN and creatinine levels, and hydronephrosis with a concomitant inflammatory response that is followed by glomerular and tubular dilation. (Cook et al., 2001.)

Development

This paracentric inversion was identified in a male offspring of a C57BL/6J female bred to a DBA/2J male that had received 1000 R cesium exposure. That male was bred to a C57BL/6J female and the strain was eventually maintained by sibling intercrossing homozygotes. Embryos were generated for cryopreservation by crossing homozygous males with C57BL/6J females.

Allele

Symbol: Uoxⁱⁿ
Name: urate oxidase In(3)55Rk-induced
MGI accession ID: MGI:2157909
Generation method: Radiation induced
Marker symbol: Uox
Marker name: urate oxidase
Chromosome: 3
Strain of origin: DBA/2J
Molecular note: PCR analysis on genomic DNA derived from mice carrying the In(3)55K inversion suggests that a disruption 3' to base 844 of exon 7 results in the mutation. Northern blot analysis on mRNA derived from liver RNA of homozygous mice confirmed that negligible amounts of encoded transcript was produced from this allele.
General note: See also In(3)55Rk-d and In(3)55Rk-p.

Allele

MGI accession ID: MGI:103742
Marker symbol: In(3)55Rk-p
Marker name: inversion, Chr 3, Roderick 55, proximal
Chromosome: 3

Allele

MGI accession ID: MGI:1333475
Marker symbol: In(3)55Rk-d
Marker name: inversion, Chr 3, Roderick 55, distal
Chromosome: 3