B6.Cg-Ldha^b/J

Stock number: 001373

Detailed description

These congenic mice carry a mobility variant of lactate dehydrogenase A. Homozygous mice are fertile and show no apparent deleterious effects of the mutation.

Development

C57BL/6J females were bred with triethylenemelamine treated DBA/2J males and an Ldh1 mobility variant was identified in their progeny and named Ldh1^b. This mutation was imported into The Jackson Laboratory and maintained by Dr. Thomas Roderick then transferred to Dr. Beverly Paigen. This allele was backcrossed to C57BL/6J for 10 generations then intercrossed to generate a homozygous strain that was sibling mated for 21 generations before homozygous males were crossed to C57BL/6J females to generate embryos for cryopreservation in 1993. (Soares, 1977 and 1978.)

Allele

Symbol: Ldha^b
Name: mobility variant b
MGI accession ID: MGI:2384558
Generation method: Chemically induced (other)
Marker symbol: Ldha
Marker name: lactate dehydrogenase A
Chromosome: 7
Strain of origin: (C57BL/6J x DBA/2J)F1
Molecular note: This mutation results in reduced activity of the enzyme; the zymogram pattern of homozygotes for this allele shows considerably less staining intensity than that of wild-type mice.
General note: Induced by triethylenemelamine (TEM).