The initial 1984 report of the bareskin mutation by Lyon and Glenister described heterozygotes and homozygotes having similar phenotype with thickened skin with folds found at approximately 2 weeks of age and the coat being sparse at 3 weeks of age, at the end of the first hair cycle, and becoming progressively thinner leaving the adult with bare skin and some hair on the snout. Corneal opacities were also reported in some heterozygotes at approximately 2 months of age. In 1998 Shiraishi et al. described the phenotype on this C57BL/6J congenic background with hair loss beginning to show at 6 to 8 weeks of age and corneal opacity with recurrent corneal epithelial defects beginning at 2 months of age. Histological assessment showed mild inflammation in the dermal stroma with mild epithelial hyperplasia in the hair follicles and epidermis at 4 months of age, with progression to severe hypoplasia in the hair follicles and epidermis with age.
The bareskin mutation was identified by Dr. Mary Lyon in the offspring of a (C3H/HeH x 101/H)F1 male treated with 250 mg/kg ethylnitrosourea. This mutation was crossed to several linkage testing stocks but was maintained in the laboratory of Dr. Lyon primarily by crossing to (C3H/HeH x 101/H)F1. Bareskin was imported from Dr. Lyon at the MRC Radiobiology Unit into The Mouse Mutant Resource at The Jackson Laboratory in 1986 and was backcrossed onto the C57BL/6J background. In 1991 embryos were generated for cryopreservation from C57BL/6J females bred to congenic heterozygous males at generation N9 or N10.
|Allele Type||Chemically induced (ENU)|
|Allele Synonym(s)||bare skin|
|Gene Symbol and Name||Gsdma3, gasdermin A3|
|Strain of Origin||(C3H/HeH x 101/H)F1|
|Molecular Note||This mutation comprises an A to G transition at nucleotide position 1113 changing tyrosine 344 to cysteine.|
When maintaining a live colony, these mice can be bred as homozygotes. The phenotype of sparse fur can be recognized at 2 weeks of age. Adults may develop skin lesions.
When using the bareskin mouse strain in a publication, please cite the originating article(s) and include JAX stock #001368 in your Materials and Methods section.