This triple congenic is useful as a cellular marker in transplantation studies in conjunction with C57BL/6J inbred mice (Ighb, Thy1b, Gpi1b). Igh is a B cell specific marker, Thy1 is a T cell specific surface marker, and Gpi1 is an enzyme that is widely expressed in mouse tissues, cultured fibroblasts, and in several types of tumors.
Read More +Genetic Background | Generation |
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000664 C57BL/6J |
F94
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Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Gpi1 | glucose phosphate isomerase 1 |
Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Thy1 | thymus cell antigen 1, theta |
Marker Symbol | Marker Name | |
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Igh | immunoglobulin heavy chain complex |
This congenic strain was made by mating together, not backcrossing, three individual congenic strains carrying differential alleles already on a C57BL/6J genetic background (B6.C-Igha, B6.PL-Thy1a, and B6.CAST-Gpi1a). This triple congenic is useful as a cellular marker in transplantation studies in conjunction with C57BL/6J inbred mice (Ighb, Thy1b, Gpi1b). Igh is a B cell specific marker, Thy1 is a T cell specific surface marker, and Gpi1 is an enzyme that is widely expressed in mouse tissues, cultured fibroblasts, and in several types of tumors.
Homozygous mice exhibit a haplotype in peripheral blood [view data; July 2020].
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | a variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | |
Gene Symbol and Name | Gpi1, glucose phosphate isomerase 1 |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 7 |
Molecular Note | This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains 101/H, A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1b. Two differences are silent, while a third difference at position nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted. |
Allele Name | a variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a |
Gene Symbol and Name | Thy1, thymus cell antigen 1, theta |
Gene Synonym(s) | |
Site of Expression | The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. |
Strain of Origin | Not Applicable |
Chromosome | 9 |
General Note | The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900). |
Molecular Note | The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains. |
Marker Synonym(s) | |
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Chromosome(s) | 12 |
When using the B6.Cg-Gpi1a Thy1a Igha/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001317 in your Materials and Methods section.
Service/Product | Description | Price |
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Homozygous for Igh<a>, Homozygous for Thy1<a>, Homozygous for Gpi1<a>, 1 pair minimum |
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