Overview

This triple congenic is useful as a cellular marker in transplantation studies in conjunction with C57BL/6J inbred mice (Igh^b, Thy1^b, Gpi1^b). Igh is a B cell specific marker, Thy1 is a T cell specific surface marker, and Gpi1 is an enzyme that is widely expressed in mouse tissues, cultured fibroblasts, and in several types of tumors.

Genetic overview

Genetic Background	Generation
000664 C57BL/6J	F94 (2021-01-11 00:00:00)

Gpi1^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	Gpi1	glucose phosphate isomerase 1

Thy1^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	Thy1	thymus cell antigen 1, theta

Marker(s)

	Marker Symbol	Marker Name
	Igh	immunoglobulin heavy chain complex

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female

View Price List

Details

Detailed Description

This congenic strain was made by mating together, not backcrossing, three individual congenic strains carrying differential alleles already on a C57BL/6J genetic background (B6.C-Igh^a, B6.PL-Thy1^a, and B6.CAST-Gpi1^a). This triple congenic is useful as a cellular marker in transplantation studies in conjunction with C57BL/6J inbred mice (Igh^b, Thy1^b, Gpi1^b). Igh is a B cell specific marker, Thy1 is a T cell specific surface marker, and Gpi1 is an enzyme that is widely expressed in mouse tissues, cultured fibroblasts, and in several types of tumors.

Homozygous mice exhibit ^a haplotype in peripheral blood [view data; July 2020].

Development

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Fabien N; Bergerot I; Maguer-Satta V; Orgiazzi J; Thivolet C. 1995. Pancreatic lymph nodes are early targets of T cells during adoptive transfer of diabetes in NOD mice. J Autoimmun 8(3):323-34PubMed: 7575994 MGI: J:26213
Pearce SR; Morgan MJ; Ball S; Peters J; Faik P. 1995. Sequence characterization of alleles Gpi1-Sa and Gpi1-Sb at the glucose phosphate isomerase structural locus. Mamm Genome 6(8):537-9PubMed: 8589524 MGI: J:28033
Trowbridge IS; Mazauskas C. 1976. Immunological properties of murine thymus-dependent lymphocyte surface glycoproteins. Eur J Immunol 6(8):557-62PubMed: 61878 MGI: J:5707

View All References

Genetics

Gpi1^a

Allele Symbol: Gpi1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)
Gene Symbol and Name	Gpi1, glucose phosphate isomerase 1
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	7
Molecular Note	This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains 101/H, A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1^b. Two differences are silent, while a third difference at position nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted.

Thy1^a

Allele Symbol: Thy1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a
Gene Symbol and Name	Thy1, thymus cell antigen 1, theta
Gene Synonym(s)
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Strain of Origin	Not Applicable
Chromosome	9
General Note	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1^b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).
Molecular Note	The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.

Igh

Marker Symbol: Igh

Marker Synonym(s)
Chromosome(s)	12

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - B cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: B cell specific marker
  - Tissue/Cell Markers

Genotype: Gpi1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Genetics Research
  - Tissue/Cell Markers

Genotype: Thy1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers

Mammalian Phenotype Terms by Genotype

References

Fabien N; Bergerot I; Maguer-Satta V; Orgiazzi J; Thivolet C. 1995. Pancreatic lymph nodes are early targets of T cells during adoptive transfer of diabetes in NOD mice. J Autoimmun 8(3):323-34PubMed: 7575994 MGI: J:26213
Pearce SR; Morgan MJ; Ball S; Peters J; Faik P. 1995. Sequence characterization of alleles Gpi1-Sa and Gpi1-Sb at the glucose phosphate isomerase structural locus. Mamm Genome 6(8):537-9PubMed: 8589524 MGI: J:28033
Trowbridge IS; Mazauskas C. 1976. Immunological properties of murine thymus-dependent lymphocyte surface glycoproteins. Eur J Immunol 6(8):557-62PubMed: 61878 MGI: J:5707

Additional - Gpi1^a related

Additional - Igh related

Additional - Thy1^a related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- End Point Analysis:Thy1 End Point
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Mating System
- Homozygote x Homozygote
Appearance
- black
  Related Genotype: a/a
Citation
When using the B6.Cg-Gpi1^a Thy1^a Igh^a/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001317 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

FGB29 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Igh<a>, Homozygous for Thy1<a>, Homozygous for Gpi1<a>, 1 pair minimum

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Genetic overview

Marker(s)

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Gpi1a

Allele Symbol: Gpi1a

Thy1a

Allele Symbol: Thy1a

Igh

Marker Symbol: Igh

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Gpi1a related

Genotype: Thy1a related

Mammalian Phenotype Terms by Genotype

References

Additional - Gpi1a related

Additional - Igh related

Additional - Thy1a related

Genotyping Protocols

Dietary Information

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Gpi1^a

Allele Symbol: Gpi1^a

Thy1^a

Allele Symbol: Thy1^a

Genotype: Gpi1^a related

Genotype: Thy1^a related

Additional - Gpi1^a related

Additional - Thy1^a related