Mice Homozygous for the subtle gray mutation have a moderate platelet storage pool deficiency and have decreased yellow hair pigment, although the black pigment does not appear affected. This is most evident in the juvenile coat of an agouti background mouse, while on a non-agouti (black) background the difference is difficult to discern and is noted by some reduction in the yellow hair pigment in the ear pinnae. Both female and male homozygotes have an intermediate elevated bleed time of 7.5 minutes compared with 2.2 minutes in heterozygous controls and more than 15 minutes in many other platelet storage pool deficiency models. Subtle gray homozygotes also have moderate deficiencies in platelet dense granules including a 20% reduction in dense granules per platelet, decreased platelet serotonin, decreased collagen-stimulated ATP release from platelets, and decreased platelet aggregation (Swank et al., 1996). These deficiencies are mild compared with those of other platelet storage pool deficiency models, with the subtle gray decrease in platelet serotonin being the most mild of any of the 12 models comparatively assessed by Swank et al (1998). No defects were found in the activities of beta-glucuronidase or beta-galactosidase, lysosomal enzymes of the kidney, distinguishing this from other platelet storage pool disease models such as mocha, muted, light ear, pale ear, palid or pearl (Swank et al., 1996). The subtlety in the dilution of coat color, the shorter bleed time and less severe platelet defects make this a less severe model than other platelet storage pool deficiency models.

The Clcc1^m1J spontaneous mutation, which causes increased sensitivity to endoplasmic reticulum stress in the cerebellum, was identified in C3H/HeSnJ (Jia et al., 2015) and has been found homozygous in all C3H/HeSn strains assessed at The Jackson Laboratory, including this mutant subline, which is homozygous for Clcc1^m1J.

Development

Subtle gray arose spontaneously in July 1985 in the C3H/HeDiSn-Dscam^2J colony at The Jackson Laboratory. The Dscam^2J mutation arose spontaneously in 1981 on the C3H/HeDiSn background at The Jackson Laboratory and was maintained by sibling mating until 1983 when hysterectomy rederivation was performed with a homozygous ovarian transplanted female bred to a C3H/HeSnJ male, the progeny of which were maintained by sibling mating. C3H/HeDiSn is approximately equivalent to its more evolved progeny C3H/HeSnJ inbred strain. The subtle gray mutation was cryopreserved on this C3H/HeSnJ background in 1986 when this strain was at generation F3.

Selected References

Nguyen T; Wei ML. 2004. Characterization of melanosomes in murine Hermansky-Pudlak syndrome: mechanisms of hypopigmentation. J Invest Dermatol 122(2):452-60PubMed: 15009730 MGI: J:88797
Swank RT; Novak EK; McGarry MP; Rusiniak ME; Feng L. 1998. Mouse models of Hermansky Pudlak syndrome: a review. Pigment Cell Res 11(2):60-80PubMed: 9585243 MGI: J:88018
Swank RT; Reddington M; Novak EK. 1996. Inherited prolonged bleeding time and platelet storage pool deficiency in the subtle gray (sut) mouse. Lab Anim Sci 46(1):56-60PubMed: 8699821 MGI: J:31616

View All References

Genetics

Slc7a11^sut

Allele Symbol: Slc7a11^sut

Allele Name	subtle gray
Allele Type	Spontaneous
Allele Synonym(s)	sut
Gene Symbol and Name	Slc7a11, solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
Gene Synonym(s)
Strain of Origin	C3H/HeSnJ
Chromosome	3
Molecular Note	Sequencing revealed a large deletion extending from intron 11 through exon 12 and into the intergenic region, creating a new splice site and the replacement of exon 12 with exon 12'. 3' RACE revealed a new stop codon in exon 12' and predicted a modified and truncated protein carboxyl terminus.

Disease/Phenotype

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Slc7a11^sut related

Dermatology Research
- Color and White Spotting Defects
Mouse/Human Gene Homologs
- Hermansky-Pudlak syndrome

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Slc7a11^sut/Slc7a11^sut
C3H/HeSnJ-Slc7a11/J

hematopoietic system phenotype

decreased platelet serotonin level
- platelet serotonin concentration is slightly reduced; no change in adenine nucleotide concentration

homeostasis/metabolism phenotype

decreased platelet serotonin level
- platelet serotonin concentration is slightly reduced; no change in adenine nucleotide concentration

increased bleeding time
- model for mild storage pool deficiency
- mutant mice have a 3.4 fold greater bleeding time compared to sib controls

integument phenotype

diluted coat color
- most easily seen with agouti especially in the juvenile coat
- on nonagouti some reduction in yellow pigment can be seen in the pinna hairs only
- yellow hair pigment is reduced; black pigment is unaffected

nervous system phenotype

decreased platelet serotonin level
- platelet serotonin concentration is slightly reduced; no change in adenine nucleotide concentration

pigmentation phenotype

diluted coat color
- most easily seen with agouti especially in the juvenile coat
- on nonagouti some reduction in yellow pigment can be seen in the pinna hairs only
- yellow hair pigment is reduced; black pigment is unaffected

References

Nguyen T; Wei ML. 2004. Characterization of melanosomes in murine Hermansky-Pudlak syndrome: mechanisms of hypopigmentation. J Invest Dermatol 122(2):452-60PubMed: 15009730 MGI: J:88797
Swank RT; Novak EK; McGarry MP; Rusiniak ME; Feng L. 1998. Mouse models of Hermansky Pudlak syndrome: a review. Pigment Cell Res 11(2):60-80PubMed: 9585243 MGI: J:88018
Swank RT; Reddington M; Novak EK. 1996. Inherited prolonged bleeding time and platelet storage pool deficiency in the subtle gray (sut) mouse. Lab Anim Sci 46(1):56-60PubMed: 8699821 MGI: J:31616

Additional - Slc7a11^sut related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the subtle gray mouse strain in a publication, please cite the originating article(s) and include JAX stock #001310 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous or Heterozygous for Slc7a11<sut>, 1 pair minimum

Related Products and Services

Frozen Mouse Embryo	C3H/HeSnJ-Slc7a11<sut>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	C3H/HeSnJ-Slc7a11<sut>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	C3H/HeSnJ-Slc7a11<sut>/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	C3H/HeSnJ-Slc7a11<sut>/J Frozen Embryos	$3373.50

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Also Known As:subtle gray

Genetic overview

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Detailed Description