Overview

Also Known As:rump white

These mice carry the radiation-induced rump white lethal mutation (Rw) characterized by hypopigmentation in heterozygotes.

Genetic overview

Genetic Background	Generation
000664 C57BL/6J

Rw

Allele Type	Gene Symbol	Gene Name
Radiation induced	Rw	rump white, inversion

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Research Applications

Developmental Biology Research
Dermatology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Rump-white is a dominant lethal mutation from which homozygotes die in utero around embryonic day 9.5. From the earliest developmental stage assessed, E7.5, smaller size is detectable in Rw/Rw embryos. Developmental arrest is found at E7.5-E8.0 likely during or near the end of gastrulation. E7.5 embryos do not have the expanded cylindrical shape normally seen at this stage. All three germ layers form, but the mesoderm is sparse and loosely organized. A rudimentary notochordal plate can be detected in some of the larger mutant embryos by E9.5. Resorption of necrotic mutant embryos appears to be underway at this point. (Searle and Truslove, 1970; Bucan et al., 1995.)

Rump-white heterozygotes have hypopigmentation in the front digits and hindquarters including white hindlegs, white tail with the tip often pigmented, and a variable degree of white in the sacral and lumbar regions with the ventral area more consistently affected than the dorsal area. At E10.5 a normal number of melanoblasts are found emigrating from the neural crest, but at E11.5 fewer are found along the neural axis particularly in the vagel and anterior trunk areas. Although fewer in number, melanoblasts are found in a normal pattern throughout the posterior axial domain until E15.5 when a reduction is seen in the hindlimbs and tail area. A rostrocaudal migration of melanoblasts to populate the vacant regions appears to occur between E15.5 and E16.5 resulting in some pigmentation in the abdomen and lumbar area, but this migration fails to extend to the hindlimbs. As with other mouse hypopigmentation mutations, Rw/+ mice have spots of hypopigmentation in the inner ear. However, Rw/+ mice do not have structural abnormalities of the cochlea and saccule that are found in many other hypopigmented mouse mutants. Rw +/+ Kit^W-19H mice, and certain other Kit mutants in repulsion with Rw, show complementation in that they have more extensive hypopigmentation than does either contributing heterozygote alone. (Searle and Truslove, 1970; Deol, 1970; Jordan and Jackson, 2000.)

Selected References

Batchelor AL; Phillips RJ; Searle AG. 1966. A comparison of the mutagenic effectiveness of chronic neutron- and gamma-irradiation of mouse spermatogonia. Mutat Res 3(3):218-29PubMed: 5962396 MGI: J:5021

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Genetics

Rw

Allele Symbol: Rw

Allele Name	rump white
Allele Type	Radiation induced
Allele Synonym(s)
Gene Symbol and Name	Rw, rump white, inversion
Gene Synonym(s)
Strain of Origin	(C3H/HeH x 101/H)F1
Chromosome	5
Molecular Note	This phenotypic allele has been attributed to In(5)6H, a 30 cM inversion in the proximal third of chromosome 5. The proximal breakpoint (In(5)6H-p) has been localized between codons 495 and 496 of Dpp6. Dpp6 mRNA was undetected in mutant mice by Northern blot analysis. Complementation analysis indicates that the recessive embryonic lethality attributed to the inversion results from a proximal region, and it had been suggested that the disrupted Dpp6 locus was responsible. However, additional deletions of the Dpp6 gene complement the rump white phenotype and these data suggest that Dpp6 is not required for viability. An additional locus, termed Qdpr is suggested to be the locus responsible for the lethal phenotype. (J:100332). The distal breakpoint (In(5)6H-d) has been localized between Pdgfra and Kit using FISH and pulsed field gel electrophoresis (PFGE). While the distal breakpoint does not disrupt the coding sequence of any known gene, it putatively has a long range effect on the expression of Kit, resulting in depigmentation of the scarolumbar region.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rw related

Developmental Biology Research
- Embryonic Lethality (Homozygous)
Dermatology Research
- Color and White Spotting Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Rw/Rw
(C3H/HeH x 101/H)F1

mortality/aging

prenatal lethality, complete penetrance
- homozygotes die in utero by mid pregnancy

Genotype: Rw/Rw⁺
(C3H/HeH x 101/H)F1

integument phenotype

variable body spotting
- heterozygotes have white hindlegs and tails and white fur on the posterior part of the abdomen, with occasional pigmented spots in the white areas; the white area is more extensive ventrally than dorsally

pigmentation phenotype

variable body spotting
- heterozygotes have white hindlegs and tails and white fur on the posterior part of the abdomen, with occasional pigmented spots in the white areas; the white area is more extensive ventrally than dorsally

Genotype: Rw/Rw⁺
involves: 101/H * C3H/HeH

integument phenotype

white spotting
- heterozygotes have a white patch of variable shape and size at the posterior extremity of the trunk, largely on the ventral side

pigmentation phenotype

white spotting
- heterozygotes have a white patch of variable shape and size at the posterior extremity of the trunk, largely on the ventral side

References

Batchelor AL; Phillips RJ; Searle AG. 1966. A comparison of the mutagenic effectiveness of chronic neutron- and gamma-irradiation of mouse spermatogonia. Mutat Res 3(3):218-29PubMed: 5962396 MGI: J:5021

Additional References

Bucan M; Nagle DL; Hough RB; Chapman VM; Lo CW. 1995. Lethality of Rw/Rw mouse embryos during early postimplantation development. Dev Biol 168(2):307-18PubMed: 7537231 MGI: J:24365
Jordan SA; Jackson IJ. 2000. A late wave of melanoblast differentiation and rostrocaudal migration revealed in patch and rump-white embryos. Mech Dev 92(2):135-43PubMed: 10727853 MGI: J:61455
Nagle DL; Martin-DeLeon P; Hough RB; Bucan M. 1994. Structural analysis of chromosomal rearrangements associated with the developmental mutations Ph, W19H, and Rw on mouse chromosome 5. Proc Natl Acad Sci U S A 91(15):7237-41PubMed: 8041773 MGI: J:19442
Searle AG; Truslove GM. 1970. A gene triplet in the mouse. Genet Res 15(2):227-35PubMed: 5529676 MGI: J:5182
Stephenson DA; Lee KH; Nagle DL; Yen CH; Morrow A; Miller D; Chapman VM; Bucan M. 1994. Mouse rump-white mutation associated with an inversion of chromosome 5. Mamm Genome 5(6):342-8PubMed: 8043948 MGI: J:18646

Additional - Rw related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the rump white mouse strain in a publication, please cite the originating article(s) and include JAX stock #001176 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wild-type for Rw

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:rump white

Genetic overview

Research Applications

Base Price

Detailed Description

Selected References

Rw

Allele Symbol: Rw

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rw related

Mammalian Phenotype Terms by Genotype

Genotype: Rw/Rw(C3H/HeH x 101/H)F1

mortality/aging

Genotype: Rw/Rw+(C3H/HeH x 101/H)F1

integument phenotype

pigmentation phenotype

Genotype: Rw/Rw+involves: 101/H * C3H/HeH

integument phenotype

pigmentation phenotype

References

Additional References

Additional - Rw related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Genotype: Rw/Rw
(C3H/HeH x 101/H)F1

Genotype: Rw/Rw⁺
(C3H/HeH x 101/H)F1

Genotype: Rw/Rw⁺
involves: 101/H * C3H/HeH