From nucleotide sequencing of H2bm10 mRNA, the spontaneous mutation contains six nucleotide alterations in the codons for amino acids 163, 165, 167, 173, and 174. At positions 173 and 174, H2bm10 contained substitutions identical to those of H2bm4.
Melvold and Kohn identified the H2bm10 histoincompatibility in a (C57BL/6Kh x BALB/cKh)F1, with these inbreds having derived from C57BL/6By and BALB/cBy founders, and this mutation was backcrossed onto C57BL/6Kh until N10 before sibling intercrossing to homozygosity. Embryos were cryopreserved at The Jackson Laboratory in 2000 from N10F45 homozygotes.
|Allele Name||b haplotype mutation 10|
|Gene Symbol and Name||H2, histocompatibility-2, MHC|
|Strain of Origin||(C57BL/6ByKh x BALB/cByKh)F1|
|Molecular Note||The bm10 mutation consists of six nucleotide substitutions resulting in amino acid changes at codon 163 (threonine to alanine), 165 (valine to methionine), 167 (tryptophan to serine), 173 (lysine to glutamate) and 174 (asparagine to leucine).|