The wild-derived inbred strain SPRET/Ei is often used in crosses with common inbred strains to create highly polymorphic panels for genetic mapping. SPRET/Ei may be useful for studies relating to inflammatory disorders.Read More +
The wild-derived inbred strain SPRET/Ei is often used in crosses with common inbred strains to create highly polymorphic panels for genetic mapping. SPRET/Ei mice are resistant to high doses of tumor necrosis factor alpha (TNFa) (Staelens et al 2002). Mice from a C57BL/6 x SPRET/Ei F1 cross were protected from TNFa-induced arthritis and partially protected against induced allergic asthma (Staelens et al 2004). SPRET/Ei may be useful in understanding certain inflammatory diseases.
As with many other wild-derived strains, this strain is extremely sensitive to noise, vibration and any change in environment. Breeding slumps are frequent and the use of foster mothers is often necessary to sustain a litter. In an effort to expand this strain from a recent severe breeding depression the diet was changed to a 10% fat grain. Subsequent to the use of the high fat diet some mice of this strain are found to develop large fat deposits on their neck and some develop long toenails as they age.
In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).
SPRET was derived from wild mice trapped by RD Sage in 1978 in Puerto Real, Cadiz Province, Spain. Mice were sent to Dr Mike Potter at The National Cancer Institute who subsequently transferred mice to Dr Eva Eicher at The Jackson Laboratory in 1983.
|Allele Name||b-3 variant|
|Allele Type||Not Applicable|
|Gene Symbol and Name||Ahr, aryl-hydrocarbon receptor|
|Strain of Origin||M. spretus|
|General Note|| |
Strain of origin - this allele was found in M. caroli, M. spretus, MOLF/Ei strains
|Molecular Note||This allele encodes a high affinity, 105 kDa receptor with slightly more heat stability than the receptor encoded by the BALB/cBy allele.|
|Allele Type||Not Applicable (Not Specified)|
|Gene Symbol and Name||Cox7a2l, cytochrome c oxidase subunit 7A2 like|
|Strain of Origin||multiple strains|
|General Note||Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T+ Itpr3tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.|
|Molecular Note||This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd.|
Due to the increased propensity of mothers of this strain to neglect their pups it is recommended that litters be fostered when feasible, particularly if pups do not display a milk spot or the nest is not maintained. Nesting material should be supplied, and the colony maintained in a quiet area with minimal disturbance. Subsequent to a severe breeding slump in 2015 this strain was moved onto grain with 10% fat.
When using the SPRET/EiJ mouse strain in a publication, please include JAX stock #001146 in your Materials and Methods section.