WSB/EiJ

Stock number: 001145
Research areas: Dermatology Research, Research Tools

Description

Watkins Star Line B (WSB) was derived from wild mice trapped in Eastern Shore, Maryland. Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

Detailed description

Watkins Star Line B (WSB) was derived from wild mice trapped in Eastern Shore, Maryland. WSB is characterized by a distinct and consistent white head blaze, variable ventral spotting and a slight coat dilution on the agouti background. The variation in pigment is the result of the spontaneous mutation blaze (Kitl^blz).

Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

Development

Founder mice for the wild-derived strain Watkins Star Line B (WSB) were trapped in Centerville, Maryland by Michael Potter (NCI) in 1976. WSB and WSA were separated from the founder line, CLA, at generation 3. The WSB, comprised mice selected for dark agouti coat color and white head blaze, Mice were sent to EM Eicher at The Jackson Laboratory in 1986. The blaze pigmentation was found to be a Kit allele, Kitl^sl-blz.

Allele

Symbol: A
Name: wild-type agouti
MGI accession ID: MGI:1856464
Generation method: Spontaneous
Synonyms: dark-bellied agouti
Marker symbol: a
Marker name: nonagouti
Marker synonyms: agouti; agouti signal protein; AGSW; AGTI; AGTIL; As; ASP; SHEP9
Chromosome: 2
Strain of origin: various
Molecular note: This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene.
General note: The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back.

Allele

Symbol: Kitl^sl-blz
Name: blaze
MGI accession ID: MGI:4431000
Generation method: Spontaneous
Marker symbol: Kitl
Marker name: kit ligand
Marker synonyms: blz; DCUA; DFNA69; FPH2; FPHH; Gb; grizzle-belly; Kitlg; KL-1; Mgf; SCF; SF; SHEP7; Sl; SLF; Steel; Steel factor; stem cell factor; WS2F
Chromosome: 10
Strain of origin: WSB/Pt
Molecular note: Complementation tests with Kitl^Sl, Kitl^Sl-d, and Kitl^sl-24J showed blaze (blz) to be an allele of Kitl. The molecular lesion is undefined.

Allele

Symbol: Cox7a2l^l
Name: long
MGI accession ID: MGI:6276121
Generation method: Not Applicable
Attributes: Not Specified
Synonyms: SCAF1¹¹³
Marker symbol: Cox7a2l
Marker name: cytochrome c oxidase subunit 7A2 like
Marker synonyms: COX7AR; COX7RP; EB1; SCAF1; SCAFI; SIG81; SIG-81
Chromosome: 17
Strain of origin: multiple strains
Molecular note: This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd.
General note: Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T⁺ Itpr3^tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.