Overview

Watkins Star Line B (WSB) was derived from wild mice trapped in Eastern Shore, Maryland. Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

Genetic overview

Genetic Background	Generation
	F61+F26 (2021-04-19 00:00:00)

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Research Applications

Dermatology Research
Research Tools

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Base Price

Starting at:

$218.00 Domestic price for female 5-week

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Details

Detailed Description

Watkins Star Line B (WSB) was derived from wild mice trapped in Eastern Shore, Maryland. WSB is characterized by a distinct and consistent white head blaze, variable ventral spotting and a slight coat dilution on the agouti background. The variation in pigment is the result of the spontaneous mutation blaze (Kitl^blz).

Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

Development

Founder mice for the wild-derived strain Watkins Star Line B (WSB) were trapped in Centerville, Maryland by Michael Potter (NCI) in 1976. WSB and WSA were separated from the founder line, CLA, at generation 3. The WSB, comprised mice selected for dark agouti coat color and white head blaze, Mice were sent to EM Eicher at The Jackson Laboratory in 1986. The blaze pigmentation was found to be a Kit allele, Kitl^sl-blz.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. Proc Natl Acad Sci U S A 114(34):9158-9163PubMed: 28784771 MGI: J:244295

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Genetics

A

Allele Symbol: A

Allele Name	wild-type agouti
Allele Type	Spontaneous
Allele Synonym(s)	dark-bellied agouti
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	various
Chromosome	2
General Note	The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back.
Molecular Note	This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene.

Kitl^sl-blz

Allele Symbol: Kitl^sl-blz

Allele Name	blaze
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Kitl, kit ligand
Gene Synonym(s)
Strain of Origin	WSB/Pt
Chromosome	10
Molecular Note	Complementation tests with Kitl^Sl, Kitl^Sl-d, and Kitl^sl-24J showed blaze (blz) to be an allele of Kitl. The molecular lesion is undefined.

Cox7a2l^l

Allele Symbol: Cox7a2l^l

Allele Name	long
Allele Type	Not Applicable (Not Specified)
Allele Synonym(s)	SCAF1¹¹³
Gene Symbol and Name	Cox7a2l, cytochrome c oxidase subunit 7A2 like
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	17
General Note	Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T⁺ Itpr3^tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.
Molecular Note	This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Dermatology Research
- Color and White Spotting Defects
Research Tools
- Genetics Research
  - Evolution and Systematics Research
  - Gene Mapping

Mammalian Phenotype Terms by Genotype

Phenotype Information

References

Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. Proc Natl Acad Sci U S A 114(34):9158-9163PubMed: 28784771 MGI: J:244295

Additional References

Aylor DL; Valdar W; Foulds-Mathes W; Buus RJ; Verdugo RA; Baric RS; Ferris MT; Frelinger JA; Heise M; Frieman MB; Gralinski LE; Bell TA; Didion JD; Hua K; Nehrenberg DL; Powell CL; Steigerwalt J; Xie Y; Kelada SN; Collins FS; Yang IV; Schwartz DA; Branstetter LA; Chesler EJ; Miller DR; Spence J; Liu EY; McMillan L; Sarkar A; Wang J; Wang W; Zhang Q; Broman KW; Korstanje R; Durrant C; Mott R; Iraqi FA; Pomp D; Threadgill D; de Villena FP; Churchill GA. 2011. Genetic analysis of complex traits in the emerging Collaborative Cross. Genome Res 21(8):1213-22PubMed: 21406540 MGI: J:169740
Keane TM; Goodstadt L; Danecek P; White MA; Wong K; Yalcin B; Heger A; Agam A; Slater G; Goodson M; Furlotte NA; Eskin E; Nellaker C; Whitley H; Cleak J; Janowitz D; Hernandez-Pliego P; Edwards A; Belgard TG; Oliver PL; McIntyre RE; Bhomra A; Nicod J; Gan X; Yuan W; van der Weyden L; Steward CA; Bala S; Stalker J; Mott R; Durbin R; Jackson IJ; Czechanski A; Guerra-Assuncao JA; Donahue LR; Reinholdt LG; Payseur BA; Ponting CP; Birney E; Flint J; Adams DJ. 2011. Mouse genomic variation and its effect on phenotypes and gene regulation. Nature 477(7364):289-94PubMed: 21921910 MGI: J:177037

Additional - A related

Additional - Cox7a2l^l related

Additional - Kitl^sl-blz related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:GAL Control Validation
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

These mice are very difficult to handle.
- Additional Breeding and Husbandry Support
Mating System
- Sibling x Sibling
Appearance
- agouti with head blaze, greyish coat
  Related Genotype: A/A
Citation
When using the WSB/EiJ mouse strain in a publication, please include JAX stock #001145 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

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Live Mouse

Age

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Price

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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