STOCK Kitl^Sl-16J/J

Stock number: 000979
Product name: steel 16 Jackson
Research areas: Cancer Research, Dermatology Research, Developmental Biology Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Neurobiology Research, Reproductive Biology Research, Research Tools, Sensorineural Research

Description

These mice carry a spontaneous remutation at the Kitl locus characterized by deficiencies in pigment cells, germ cells, and blood cells.

Detailed description

The steel mutations cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations. Most steel homozygous mice are severely anemic in utero and die usually at 15 to 16 days of gestation. However, compounds of two Mgf^Sl mutants (e.g. Mgf^Sl/Mgf^Sl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable Mgf^Sl/ Mgf^Sl homozygotes and deficient in the Mgf^Sl/+ heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age.

Development

The Steel 16 Jackson mutation arose spontaneously in a stock derived from a 129 female crossed with a male of a wild-derived line originating from Lipari, Italy, which had been imported from Dr. Alfred Gropp into the laboratory of Dr. Eva Eicher at The Jackson Laboratory. This mutant subline was crossed once to B6D2, once to C57BL/6J and then twice to AEJ/GnRk and then maintained by sibling breeding thereafter, resulting in a nonagouti STOCK. In 1984 embryos were cryopreserved from C57BL/6J females bred to heterozygous males at generation F16.

Allele

Symbol: Kitl^Sl-16J
Name: steel 16 Jackson
MGI accession ID: MGI:1856171
Generation method: Spontaneous
Marker symbol: Kitl
Marker name: kit ligand
Chromosome: 10
Strain of origin: (129 x Lipari)F1