These mice carry a spontaneous remutation at the Kitl locus characterized by deficiencies in pigment cells, germ cells, and blood cells.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Kitl | kit ligand |
The steel mutations cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations. Most steel homozygous mice are severely anemic in utero and die usually at 15 to 16 days of gestation. However, compounds of two MgfSl mutants (e.g. MgfSl/MgfSl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable MgfSl/ MgfSl homozygotes and deficient in the MgfSl/+ heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age.
The Steel 16 Jackson mutation arose spontaneously in a stock derived from a 129 female crossed with a male of a wild-derived line originating from Lipari, Italy, which had been imported from Dr. Alfred Gropp into the laboratory of Dr. Eva Eicher at The Jackson Laboratory. This mutant subline was crossed once to B6D2, once to C57BL/6J and then twice to AEJ/GnRk and then maintained by sibling breeding thereafter, resulting in a nonagouti STOCK. In 1984 embryos were cryopreserved from C57BL/6J females bred to heterozygous males at generation F16.
Allele Name | steel 16 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Kitl, kit ligand |
Gene Synonym(s) | |
Strain of Origin | (129 x Lipari)F1 |
Chromosome | 10 |
When using the steel 16 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000979 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for a, Heterozygous or Wild-type for Kitl<Sl-16J> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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