C57BL/6JEiJ

Stock number: 000924
Product name: Black 6 Eicher
Research areas: Research Tools

Description

The Eicher (Ei) C57BL/6 subline was separated from the parental C57BL/6J in 1976 and has been maintained as a separate subline since. C57BL/6 is the most widely used inbred strain. It is commonly used as a general purpose strain and background strain for the generation of congenics carrying both spontaneous and induced mutations. C57BL/6J mice are used in a variety of research areas including cardiovascular biology, developmental biology, diabetes and obesity, genetics, immunology, neurobiology, and sensorineural research.

Detailed description

The Eicher (Ei) C57BL/6 subline was separated from the parental C57BL/6J in 1976 and has been maintained as a separate subline since. C57BL/6 is the most widely used inbred strain. It is commonly used as a general purpose strain and background strain for the generation of congenics carrying both spontaneous and induced mutations. Although this strain is refractory to many tumors, it is a permissive background for maximal expression of most mutations. C57BL/6J mice are used in a wide variety of research areas including cardiovascular biology, developmental biology, diabetes and obesity, genetics, immunology, neurobiology, and sensorineural research. C57BL/6J mice are also commonly used in the production of transgenic mice. Overall, C57BL/6 mice breed well, are long-lived, and have a low susceptibility to tumors.

This subline differs from C57BL/6J at one known locus, although there may be others that have not been identifed. C57BL/6JEiJ does not have a spontaneous deletion in nicotinamide nucleotide transhydrogenase (Nnt), but does have the n-Tr20^m1J mutation.

Development

The Eicher subline separated from the parental C57BL/6J in 1976 when that inbred was at generation F129. It has been maintained as a separately inbreeding subline ever since.

Allele

Symbol: n-TRtct5^m1J
Name: mutation 1, Jackson
MGI accession ID: MGI:5575489
Generation method: Spontaneous
Attributes: Not Specified
Synonyms: n-Tr20^C57BL/6J; n-TRtct5^rs4644711-T; rs46447118
Marker symbol: n-TRtct5
Marker name: nuclear encoded tRNA arginine 5 (anticodon TCT)
Marker synonyms: trna730-ArgTCT
Chromosome: 1
Strain of origin: C57BL/6J
Molecular note: This C-to-T transition, the T variant of SNP rs46447118, found in C57BL/6J and C57BL/6JEiJ but not any of the other 18 inbred strains tested, is in nucleotide 50 in the stem of the T loop and impairs maturation, leading to accumulation of a 105 nucleotide pre-tRNA band with leader and trailer sequences instead of the usual 115 nucleotide pre-tRNA, and also diminishes aminoacylation of this isodecoder.