In(X)1H is a long inversion in the X Chromosome that is valuable in the study of chromosomal pairing, meiotic non-disjunction, imprinting and X Chromosome inactivation, as well the generation of X/O mice which can model Turner syndrome.Read More +
In(X)1H is a long paracentric inversion involving approximately 85% of the X Chromosome with the proximal breakpoint originally reported by Evans and Phillips (1975) to be just within the dark centric band A1 and the distal breakpoint found distal to band E. This strain can be maintained by breeding homozygous females with hemizygous males, but litter size is smaller than normal. When breeding involves heterozygous females there is a significant number of X/O mice produced because of meiotic non-disjunction. O/Y are not viable in mice, but X/O generally are. This strain is valuable in the study of chromosomal pairing, meiotic non-disjunction, imprinting and X Chromosome inactivation, as well the generation of X/O mice which can model Turner syndrome.
In(X)1H was initially identified associated with the NsdhlBpa mutation in the progeny of an irradiated male (C3H/HeH x 101/H)F1. The subsequent breeding protocol at Harwell is not currently known, but NsdhlBpa was bred out before the strain was imported from Harwell into the laboratories of Dr. Eva Eicher and Dr. Tom Roderick in the mid-1970s. After 17 generations of sibling intercrossing this strain was backcrossed twice to either C57BL/6J or C57BL/6JEi in the laboratory of Dr. Eva Eicher in 1981 and the strain was subsequently sibling intercrossed. Embryos were generated for cryopreservation from N2F4 hemizygous males bred to either C57BL/6J or C57BL/6JEi females in 1982.
This strain can be maintained by breeding homozygous females with hemizygous males, but productivity is low. Significant production of XO females has been identified at least from breeding schemes that include a wild-type X Chromosome.
When using the STOCK In(X)1H/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000919 in your Materials and Methods section.