C.B6-Tyr⁺ Hbb^s/J

Stock number: 000899
Research areas: Dermatology Research, Hematological Research, Mouse/Human Gene Homologs

Description

The Hbb^s allele of C57BL/6J has been introgressed into the BALB/c strain to create this congenic strain.

Detailed description

In this congenic strain, the s allele has been introgressed into the BALB/c strain (normally Hbb^d). The hemoglobin beta globin allele s occurs naturally in the C57BL/6 inbred strain. Mice with the S haplotype (Hbb^s/Hbb^s) have two s alleles. Beta globins synthesized from these alleles dimerize and migrate together with alpha globin to form a single band during electrophoresis. The s beta globin is similar to human beta globin, but differs from other mouse beta globins (d, p) by the presence of a single reactive cysteine residue at position 93 (betaCys93). BetaCys93 is involved in nitric oxide metabolism and the formation of mixed disulfides with glutathione and may affect susceptibility to pathogenic infection.

Development

The s allele has been identified as a glycine (GGC) at position 13 and a cysteine at position 93. In contrast, the d allele is comprised of a cysteine at both positions. The Hbb^s allele of C57BL/6J has been introgressed into the BALB/c strain to create this congenic strain.

Allele

Symbol: Hbb^s
Name: single
MGI accession ID: MGI:2677684
Generation method: Not Applicable
Marker symbol: Hbb
Marker name: hemoglobin beta chain complex
Chromosome: 7
Strain of origin: C57BL/6
Molecular note: This natural variant identified by protein sequencing, produces a single beta globin with one reactive cysteine (betaCyst13). Two structurally identical beta globins migrate during electrophoresis in a dimer with alpha globin as a single band, therefore the s designation.

Allele

Symbol: Tyr⁺
Name: wild type
MGI accession ID: MGI:2432472
Generation method: Not Applicable
Marker symbol: Tyr
Marker name: tyrosinase
Chromosome: 7
Strain of origin: Not Applicable