In this congenic strain, the s allele has been introgressed into the BALB/c strain (normally Hbbd). The hemoglobin beta globin allele s occurs naturally in the C57BL/6 inbred strain. Mice with the S haplotype (Hbbs/Hbbs) have two s alleles. Beta globins synthesized from these alleles dimerize and migrate together with alpha globin to form a single band during electrophoresis. The s beta globin is similar to human beta globin, but differs from other mouse beta globins (d, p) by the presence of a single reactive cysteine residue at position 93 (betaCys93). BetaCys93 is involved in nitric oxide metabolism and the formation of mixed disulfides with glutathione and may affect susceptibility to pathogenic infection.
The s allele has been identified as a glycine (GGC) at position 13 and a cysteine at position 93. In contrast, the d allele is comprised of a cysteine at both positions.
The Hbbs allele of C57BL/6J has been introgressed into the BALB/c strain to create this congenic strain.
|Allele Type||Not Applicable|
|Gene Symbol and Name||Hbb, hemoglobin beta chain complex|
|Strain of Origin||C57BL/6|
|Molecular Note||This natural variant identified by protein sequencing, produces a single beta globin with one reactive cysteine (betaCyst13). Two structurally identical beta globins migrate during electrophoresis in a dimer with alpha globin as a single band, therefore the s designation.|
When using the C.B6-Tyr+ Hbbs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000899 in your Materials and Methods section.