HPG/BmJ

Stock number: 000804
Research areas: Endocrine Deficiency Research, Mouse/Human Gene Homologs, Reproductive Biology Research, Sensorineural Research

Description

This strain carries the hypogonadal mutation (Gnrh1^hpg) and mice are characterized by an overall underdevelopment of the reproductive tract.

Detailed description

Mice homozygous for the hypogonadal mutation (Gnrh1^hpg) are characterized by an overall underdevelopment of the reproductive tract. Male mice have a small penis and scrotum and a short ano-genital distance. All male reproductive organs are present but are immature. The testes are small and undescended. Spermatogenesis is arrested, usually by the diplotene stage. The vagina of female mice does not open fully, the uterus is thread-like, and the ovaries are very small. Homozygous mutant mice are deficient in LH, FSH, and gonadal steroids. They also exhibit aberrant PRL secretion patterns. Both sexes are sterile although fertility can be restored with hormone replacement.

Development

Origin: WG Beamer, 1988 following hysterectomy and fostering on B6D2 of a stock carrying hpg (hypogonadal) mutation obtained from Bruce Cattanach.

Male and female mice heterozygous for Gnrh1^hpg were mated to produce embryos for cryopreservation in 2008.

Allele

Symbol: Pde6b^rd1
Name: retinal degeneration 1
MGI accession ID: MGI:1856373
Generation method: Spontaneous
Marker symbol: Pde6b
Marker name: phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Chromosome: 5
Strain of origin: various
Molecular note: Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C-to-A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain, has been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested.
General note: The following inbred strains are known to be homozygous for Pde6b: C3H sublines, CBA/J, FVB/NJ, PL/J, SB, SJL/J, and SWR/J.

Allele

Symbol: Gnrh1^hpg
Name: hypogonadal
MGI accession ID: MGI:1856393
Generation method: Spontaneous
Marker symbol: Gnrh1
Marker name: gonadotropin releasing hormone 1
Chromosome: 14
Strain of origin: C3H101H-Rb(1.3)1Bnr
Molecular note: The mutation in the hypogonadal mouse is an approximately 33.5 kb deletion that removes two exons that encode most of the protein.