These mice carry a spontaneous mutation at the Dnah11 locus characterized by inverse placement of the visceral and thoracic organs and in anomalous positioning and interactions of blood vessels. They are suitable for use in applications related to the study of abnormal laterality in humans.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Dnah11 | dynein, axonemal, heavy chain 11 |
DNAH11 is important for developmental control of organ positioning in the left-right axis such that homozygosity for the situs inversus viscerum (iv) mutant allele can result not only in inverse placement of the visceral and thoracic organs, but also in anomalous positioning and interactions of blood vessels (including the hepatic portal, inferior vena cava, and azygos vein) and modified shape of organs and blood vessels, including abnormal lobation of lungs or liver. Approximately 50% of mice homozygous for Dnah11iv have situs inversus, and the likelihood of situs inversus is not impacted by whether the homozygous parent has situs inversus. This indicates that wild type Dnah11 instructs left-right asymmetry, and in the absence of functional Dnah11 the direction of this asymmetry is random. Heterotaxia is found in less than half of homozygotes and occurs equally in those that do and do not have situs inversus. While heterotaxia may be impacted by genetic background, the incidence of situs inversus has not shown this variation. Situs inversus can be identified shortly after birth, until the skin thickens at approximately day 5, by viewing the location of the milk-filled stomach through the skin. Homozygotes are generally viable and do breed, although poorreproductive performance with a high rate of resorption has been reported by Brown et al. (Development 1989). Some premature death has been reported and may be caused by deformities of the cardiac loop. (Hummel and Chapman, 1959; Layton 1976; Brown et al., 1989; Icardo and Colvee, 2001.)
In 1956 Katherine Hummel reported finding situs inversus viscerum (iv) in 6 out of 42 mice in the F3 generation from a cross of a C3H/e female with an my/my male. This my/my male was likely from the line that was then being inbred to become My/Hu (see stock#000265). The my mutation was bred out of this new mutant stock and in 1972 the iv-bearing stock was transferred from Katherine Hummel to Robert Collins, both at The Jackson Laboratory. Collins began inbreeding from the outbred stock in 1975 and this generated the strain SI/Col (see stock#001045) which is homozygous for Dnah11iv, a, and Tyrp1b. SI/Col reached F62 in 1994. At some point the Dnah11iv mutation was bred onto 129/Sv-ter but the details of this breeding have been lost. In 1981 males homozygous for Dnah11iv on the 129/Sv-ter background were bred to C57BL/6J females to generate embryos for cryopreservation.
Allele Name | situs inversus viscerum |
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Allele Type | Spontaneous |
Allele Synonym(s) | iv |
Gene Symbol and Name | Dnah11, dynein, axonemal, heavy chain 11 |
Gene Synonym(s) | |
Strain of Origin | (C3HeB/Fe x STOCK Frem2my)F3 |
Chromosome | 12 |
Molecular Note | A G-to-A transition mutation led to a substitution of glutamate with lysine at position 2271 (p.E2271K)in the encoded protein. This residue is located between the second and third P-loop motifs, a highly conserved region that constitutes the motor domain. |
When using the situs inversus viscerum mouse strain in a publication, please cite the originating article(s) and include JAX stock #000773 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Dnahc11<iv> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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