This strain is homozygous for the retinal degeneration allele Pde6brd1.
A 7 base pair deletion was reported in the Cd4 5' untranslated region of Stock No. 000550, MOLF/EiJ (Wade et al. 1993, Capparelli et al, 2004). Subsequent testing at The Jackson Laboratory indicated that MOLC/EiJ, MOLD/EiJ and MOLG/EiJ also carry the variant sequence. The deletion is not believed to affect expression.
It was developed from wild Mus musculus molossinus without any intercrossing with laboratory mice. It differs from laboratory mice at many biochemical loci and also in skeletal morphology. It also has a low retinal ganglion cell number.
In 1969 Dr. Michael Potter at NCI sent 10 M. m. molossinus from a population that was within 1 to 3 generations of the wild population captured in Fukuoka, Kyushu, Japan to Dr. Thomas Roderick at The Jackson Laboratory. Dr. Roderick generated three inbred lines from single pairings of these mice and named them MOLC/Rk, MOLD/Rk, and MOLE/Rk. MOLC/Rk derived from the line he designated MOL 3-I, and MOLD/Rk and MOLE/Rk derived from the line designated MOL 3-III. (Roderick, 1982.)
|Allele Name||retinal degeneration 1|
|Allele Synonym(s)||Pdebrd1; rd; rd1; rd-1; rodless retina|
|Gene Symbol and Name||Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide|
|Strain of Origin||various|
|General Note||The following inbred strains are known to be homozygous for Pde6b |
|Molecular Note||Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C-to-A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain, has been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested.|
These mice are small and often need to wait until 4 weeks of age to wean.
When using the MOLD/RkJ mouse strain in a publication, please include JAX stock #000734 in your Materials and Methods section.