Dr. Thomas Roderick, The Jackson Laboratory
Genetic Background | Generation |
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|
Marker Symbol | Marker Name | |
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In(13)43Rk | inversion, Chr 13, Roderick 43 |
The In(13)43Rk mutation was radiation induced by exposing a male that likely derived from a Mus poschiavinus to 1000 rads from a cesium irradiator. This male was subsequently bred to a C57BL/6J female. This large inversion, which encompasses Ap3b1, was maintained through a breeding scheme that made use of the pearl mutation, Ap3b1pe. By breeding to a pearl homozygote this inversion was captured in repulsion with the recessive pearl mutation as a marker. By intercrossing these repulsion heterozygotes the offspring can be: homozygous for the pearl coat color mutation and thus lack the inversion; homozygous for the inversion; or heterozygous for both pearl and the inversion carried in repulsion. Any non-pearl pups can be intercrossed for a progeny test to identify those carrying the pearl mutation, and therefore repulsion heterozygotes, from those not carrying pearl and therefore homozygous for the inversion. Embryos were cryopreserved from this line from repulsion heterozygotes either intercrossed or bred to mice homozygous for the inversion. Thus, the cryorecovered pups will either be homozygous for the inversion or heterozygous for the inversion and pearl in repulsion.
Marker Synonym(s) | |
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Chromosome(s) | 13 |
When using the STOCK In(13)43Rk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000727 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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In(13)43Rk +/+ Ap3b1<pe> repulsion heterozygotes or In(13)43Rk homozygotes |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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