Overview

Also Known As:POSF

Mice of this strain are homozygous for Robertsonian translocations Rb(1.3)1Bnr, Rb(8.12)5Bnr and Rb(9.14)6Bnr. These mice are useful for production of antibody producing hybridomas.

Genetic overview

Genetic Background	Generation

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Research Applications

Research Tools
Neurobiology Research
Sensorineural Research
Endocrine Deficiency Research
Metabolism Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

This strain is homozygous for retinal degeneration 3, rd3.

Detailed Description

The RBF inbred strain arose from crosses with wild mice, originally known as "tobacco mouse", captured in Valle di Poschiavo in S.E. Switzerland. The wild mice originally known as 'tobacco mouse' because of the coat colour. The strain was transferred to Dr. M. Davisson (Dn) in 1981 and subsequently to the production colony of The Jackson Laborotory (J). Mice are homozygous for Robertsonian translocation Rb(1.3)1Bnr, Rb(8.12)5Bnr and Rb(9.14)6Bnr.

Development

RBF stands for Robertsonian (RB) POSF. POSF was a subline of M. m. domesticus poschiavinus wild derived stock, which had several Robertsonian translocations. Alfred Gropp mated feral male from the Seiss Poschiavo Valley to a Swiss strain and F1 hybrids were received by Tom Roderick at The Jackson Laboratory where the colony was subsequently transferred to Davisson. In September 1983 this strain reached generation F53.

Selected References

Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298

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Genetics

Rd3^rd3

Allele Symbol: Rd3^rd3

Allele Name	retinal degeneration 3
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Rd3, retinal degeneration 3
Gene Synonym(s)
Strain of Origin	RBF/DnJ
Chromosome	1
General Note	This mutation causes retinal degeneration. In homozygous mutant mice, development proceeds normally through the second postnatal week. Thereafter, photoreceptor and outer nuclear layers begin to degenerate, and by 8 weeks, no photoreceptor cells remain. Changes in electroretinograms parallel the histologic changes. As of 1998 this is the only type of retinal degeneration reported in mice in which the photoreceptors are initially normal (J:4367).
Molecular Note	A C to T substitution in Rd3 results in a stop codon after residue 106. The truncated protein is initially expressed in in vitro assays but is degraded.

Cdh23^ahl

Allele Symbol: Cdh23^ahl

Allele Name	age related hearing loss 1
Allele Type	Spontaneous
Allele Synonym(s)	Cdh23^753A; mdfw
Gene Symbol and Name	Cdh23, cadherin 23 (otocadherin)
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	10
Molecular Note	Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has shown this is caused by a G to A transition at coding nucleotide position 753 of Cdh23 (SNP rs257098870). This hypomorphic allele changes splice donor site G-GT to A-GT, causing frame skipping of exon 7. This is predicted to delete part of the 2nd and 3rd ectodomains and cause reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD-1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LtJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: 129S1/SvImJ, C3H/HeSnJ, I/LnJ, YBR/Ei, MRL/MpJ.

Ahr^d

Allele Symbol: Ahr^d

Allele Name	d variant
Allele Type	Not Applicable
Allele Synonym(s)	ah; Ah^d; Ah^k; Ahhⁿ; AhRd; in
Gene Symbol and Name	Ahr, aryl-hydrocarbon receptor
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	12
General Note	Strain of origin - this allele was found in DBA/2J, AKR/J, 129, SWR, RF, NZB strains
Molecular Note	This allele encodes a 104 kDa receptor that is stabilized by molybdate and has an affinity for ligand 10-100 fold lower than that of the receptor produced by the C57BL/6J allele. PCR sequencing of cDNA revealed ten nucleotide differences between the coding sequences of the DBA/2J and C57BL/6J receptors. Five of the ten differences would cause amino acid changes. One of these, an apparent T to C transition replaces the opal termination codon in the C57BL/6J allele with an arginine codon in the DBA/2J allele. This change would extend translation of the DBA/2J mRNA by 43 amino acids, accounting for the larger size of the peptide produced by this allele (104 kDa vs 95 kDa for the C57BL/6J allele). A second T to C transition changes a leucine codon in the C57BL/6J allele to a proline codon in the DBA/2J allele, and would likely change secondary structure of the peptide and thus ligand affinity.

Mc1r^E-tob

Allele Symbol: Mc1r^E-tob

Allele Name	tobacco darkening
Allele Type	Spontaneous
Allele Synonym(s)	E^tob
Gene Symbol and Name	Mc1r, melanocortin 1 receptor
Gene Synonym(s)
Strain of Origin	M. m. domesticus poschiavinus
Chromosome	8
Molecular Note	Two independent PCR assays found a C-to-T mutation at coding nucleotide 206 resulting in a serine to leucine alteration in codon 69 (p.S69L). A second nucleotide C-to-T change results in a silent mutation at position 166. The result is a receptor that is overactive, though responsive to hormone regulation, and produces a greater activation of the MC1R effector, G protein-coupled adenylyl cyclase, than does the wild-type allele.

Rb(9.14)6Bnr

Marker Symbol: Rb(9.14)6Bnr

Marker Synonym(s)
Chromosome(s)	14

Rb(8.12)5Bnr

Marker Symbol: Rb(8.12)5Bnr

Marker Synonym(s)
Chromosome(s)	12

Rb(1.3)1Bnr

Marker Symbol: Rb(1.3)1Bnr

Marker Synonym(s)
Chromosome(s)	1

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: multiple Robertsonian chromosomes
  - Mutagenesis and Transgenesis: multiple Robertsonian chromosomes
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
- Cancer Research
  - myeloma and hybridoma production
Sensorineural Research
- Retinal Degeneration
  - Homozygous for rd3
Neurobiology Research
- Hearing Defects
  - Age related hearing loss

Genotype: Cdh23^ahl related

Neurobiology Research
- Hearing Defects
  - Age related hearing loss
Sensorineural Research
- Hearing Defects
  - Age related hearing loss

Genotype: Mc1r^E-tob related

Endocrine Deficiency Research
- Skin Defects

Genotype: Rd3^rd3 related

Sensorineural Research
- Retinal Degeneration
  - Homozygous for rd3

Genotype: Ahr^d related

Metabolism Research
Research Tools
- Toxicology Research

Mammalian Phenotype Terms by Genotype

Phenotype Information

Festing Inbred Strain Characteristics: RBF

References

Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298

Additional References

Bachmanov AA; Beauchamp GK; Tordoff MG. 2002. Voluntary consumption of NaCl, KCl, CaCl2, and NH4Cl solutions by 28 mouse strains. Behav Genet 32(6):445-57PubMed: 12467342 MGI: J:80489
Bachmanov AA; Reed DR; Beauchamp GK; Tordoff MG. 2002. Food intake, water intake, and drinking spout side preference of 28 mouse strains. Behav Genet 32(6):435-43PubMed: 12467341 MGI: J:80495
Poland A; Glover E. 1990. Characterization and strain distribution pattern of the murine Ah receptor specified by the Ahd and Ahb-3 alleles. Mol Pharmacol 38(3):306-12PubMed: 2169579 MGI: J:34840
Taggart RT; Samloff IM. 1983. Stable antibody-producing murine hybridomas. Science 219(4589):1228-30PubMed: 6402815 MGI: J:135139
Zheng QY; Tong YC; Alagramam KN; Yu H. 2007. Tympanometry assessment of 61 inbred strains of mice. Hear Res 231(1-2):23-31PubMed: 17611057 MGI: J:123543

Additional - Ahr^d related

Additional - Cdh23^ahl related

Additional - Mc1r^E-tob related

Additional - Rd3^rd3 related

Additional - Rb(1.3)1Bnr related

Additional - Rb(8.12)5Bnr related

Additional - Rb(9.14)6Bnr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Breeding Considerations

This strain is a challenging breeder.
- Additional Breeding and Husbandry Support
Mating System
- Sibling x Sibling
Appearance
- albino, retinal degeneration
  Related Genotype: Tyr^c/Tyr^c Mc1r^E-tob/Mc1r^E-tob
Citation
When using the RBF/DnJ mouse strain in a publication, please include JAX stock #000726 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Inbred, 1 pair minimum will be supplied

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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