129 inbred mice are characterized by a high incidence of spontaneous testicular teratomas, though the incidence differs between substrains. 129/SvJ mice, as well as mice from other 129 sublines, are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. Major genetic variation exists between various sublines of the 129 "family".
Read More +Genetic Background | Generation |
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This strain is homozygous for Cdh23ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss with onset prior to three months of age.
Historically, the 129 inbred mice are known for the high incidence of spontaneous testicular teratomas, though the incidence differs between substrains. (1-3% in 129 parental substrains; 30% in teratoma substrains.) More recently 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. There is major genetic variation within the 129 "family", which has led to an update of the nomenclature and a division of the substrains into three major groups: parental substrains (129P), steel substrains (129S) and "teratoma" substrains (129T). Investigators using 129 substrains for targeted mutagenesis should be careful in the selection of the appropriate 129 substrain to match the embryonic stem cell line. For a complete history of the numerous 129 substrains, see Simpson et al. 1997.
In response to challenge, 129X1/SvJ mice develop immune-mediated nephritis characterized by proteinuria, glomerulonephritis and tubulointerstitial disease (Xie et al. 2004).
White et al. reported a variation in thioglycolate medium-induced peritoneal leukocyte recruitment in 4 analyzed strains. The response of total leukocyte recruitment, from greatest to least, was C57BL/6J>BALB/c>CD1>129X1/SvJ. Variations were also found in the timeline of response and cell types most impacted.
129X1/SvJ mice were transferred from the laboratory of Dr. Roy Stevens (Sv) at The Jackson Laboratory to the production colonies in 1982. Historical data and genetic analysis indicate that an accidental outcrossing of 129X1/Sv occurred prior to that transfer, sometime between 1977 and 1978, resulting in a change in coat color from Aw to a (white-bellied agouti to nonagouti) in the 129/Sv substrain.
Allele Name | age related hearing loss 1 |
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Allele Type | Spontaneous |
Allele Synonym(s) | Cdh23753A; mdfw |
Gene Symbol and Name | Cdh23, cadherin 23 (otocadherin) |
Gene Synonym(s) | |
Strain of Origin | multiple strains |
Chromosome | 10 |
Molecular Note | Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has shown this is caused by a G to A transition at coding nucleotide position 753 of Cdh23 (SNP rs257098870). This hypomorphic allele changes splice donor site G-GT to A-GT, causing frame skipping of exon 7. This is predicted to delete part of the 2nd and 3rd ectodomains and cause reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD-1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LtJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: 129S1/SvImJ, C3H/HeSnJ, I/LnJ, YBR/Ei, MRL/MpJ. |
Allele Name | polymerase iota deficient |
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Allele Type | Spontaneous (Null/Knockout) |
Allele Synonym(s) | Poli-; Poli129 |
Gene Symbol and Name | Poli, polymerase (DNA directed), iota |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 18 |
Molecular Note | A C-to-A nonsense mutation changes codon 27 from serine (TCG) to an amber stop codon (TAG) (SW:Q6R3M4 p.S27*), and results in a truncated protein lacking any catalytic function. The allele was present in every 129 strain analyzed, including 129P3/J, 129X1/SvJ, 129P1/ReJ and 129P2/Ola. C57BL/6J and BALB/c mice did not contain the mutation. |
Allele Name | deletion |
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Allele Type | Spontaneous |
Allele Synonym(s) | Disc1129S6; Disc1delta6 |
Gene Symbol and Name | Disc1, disrupted in schizophrenia 1 |
Gene Synonym(s) | |
Strain of Origin | various |
Chromosome | 8 |
General Note | This deletion appears in multiple strains of the 129 superfamily, 101/RI, BTBR T+ tf/J, LP/J, FVB/NJ, SJL/J, SWR/J and DDY/JclSidSeyFrkJ (J:111837, J:195189). |
Molecular Note | A 25 bp deletion in exon 6 causes a frame shift in the reading frame, resulting in 13 novel amino acids and a premature stop codon in exon 7. |
Allele Name | ridge |
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Allele Type | Spontaneous (Null/Knockout) |
Allele Synonym(s) | ridge |
Gene Symbol and Name | Astn2, astrotactin 2 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 4 |
Molecular Note | The molecular lesion is a a ~30 kb deletion that encompasses exon 5 and has endpoints within a pair of LINE elements. PCR typing showed that the ~30 kb deletion is present in both R1 ES cells and in 129X1/SvJ mice, but not in the closely related 129S1/SvlmJ or 129S6/SvEvTac strains. |
pink-eyed, light-bellied, light chinchilla
Related Genotype: Aw/Aw Oca2p Tyrc-ch/Oca2p Tyrc
albino
Related Genotype: Aw/Aw Oca2p Tyrc/Oca2p Tyrc
pink-eyed, light-bellied, chinchilla
Related Genotype: Aw/Aw Oca2p Tyrc-ch/Oca2p Tyrc-ch
When using the 129X1 mouse strain in a publication, please include JAX stock #000691 in your Materials and Methods section.
Service/Product | Description | Price |
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Inbred, 1 pair minimum will be supplied |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #2 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #2 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #2 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #2 mES cells | $995.00 |
Mouse ES Cells | 129X1/SvJ-PRX-129X1 #1 mES cells | $995.00 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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