JAX Mouse Strain Datasheet - 000687

SM/J

Stock No:: 000687

Inbred Strain

Repository Live

Overview

Also Known As: small

SM/J mice carry a number of rare polymorphic alleles and are often matched to other strains for quantitative trait locus analysis. These mice are susceptible to diet-induced obesity and diet-induced atherosclerosis. SM/J mice exhibit a hyperresponsiveness to B cell mitogens. Small in size at birth and through weaning, SM/J mice attain a normal body weight as they age.

Genetic overview

Genetic Background	Generation
	F222 (28-JUL-16)

View Genetics

Research Applications

Cardiovascular Research
Developmental Biology Research
Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$224.00 Domestic price for female

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Details

Detailed Description

SM/J mice carry a number of rare polymorphic alleles and are often matched to LG/J (Stock No. 000675), A/J (Stock No. 000646) or NZB/BINJ (Stock No. 000684) for quantitative trait locus analysis. These mice are susceptible to diet-induced obesity and diet-induced atherosclerosis. SM/J mice exhibit a hyperresponsiveness to B cell mitogens (Clark et al. 1981, Engel et al. 1981). A point mutation in Neu1 is responsible for a partial deficiency of lysosomal neuraminadase and may explain the altered immune response (Rottier et al. 1998). Small in size at birth and through weaning, SM/J mice attain a normal body weight as they age.

Development

SM/J, created by MacArthur in 1939 from crosses involving 7 inbred strains - among them DBA - followed by inbreeding with selection for small body size, is segregating white-bellied agouti (A^w) vs. nonagouti (a) at the agouti locus.

Selected References

Anunciado RV; Nishimura M; Mori M; Ishikawa A; Tanaka S; Horio F; Ohno T; Namikawa T. 2001. Quantitative trait loci for body weight in the intercross between SM/J and A/J mice. Exp Anim 50(4):319-24. PubMed: 11515095 MGI: J:71465
Ehrich TH; Kenney JP; Vaughn TT; Pletscher LS; Cheverud JM. 2003. Diet, obesity, and hyperglycemia in LG/J and SM/J mice. Obes Res 11(11):1400-10. PubMed: 14627762 MGI: J:86873
Korstanje R; Albers JJ; Wolfbauer G; Li R; Tu AY; Churchill GA; Paigen BJ. 2004. Quantitative trait locus mapping of genes that regulate phospholipid transfer activity in SM/J and NZB/BlNJ inbred mice. Arterioscler Thromb Vasc Biol 24(1):155-60. PubMed: 14592843 MGI: J:86340
Korstanje R; Li R; Howard T; Kelmenson P; Marshall J; Paigen B; Churchill G. 2004. Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population. J Lipid Res 45(5):881-8. PubMed: 14993241 MGI: J:89309
Macarthur JW. 1949. Selection for Small and Large Body Size in the House Mouse. Genetics 34(2):194-209. PubMed: 17247310 MGI: J:2452
Macarthur JW. 1944. Genetics of body size and related characters Am Naturalist 78:142-57. MGI: J:25351
Paigen B; Ishida BY; Verstuyft J; Winters RB; Albee D. 1990. Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice. Arteriosclerosis 10(2):316-23. PubMed: 2317166 MGI: J:22615
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11. PubMed: 15081119 MGI: J:89298
Pitman WA; Korstanje R; Churchill GA; Nicodeme E; Albers JJ; Cheung MC; Staton MA; Sampson SS; Harris S; Paigen B. 2002. Quantitative trait locus mapping of genes that regulate HDL cholesterol in SM/J and NZB/B1NJ inbred mice. Physiol Genomics 9(2):93-102. PubMed: 12006675 MGI: J:76707

View All References

Genetics

Gpr84^del

Allele Symbol: Gpr84^del

Allele Name	deletion
Allele Type	Spontaneous (Not Specified)
Allele Synonym(s)
Gene Symbol and Name	Gpr84, G protein-coupled receptor 84
Gene Synonym(s)	EX33; GPCR4
Strain of Origin	multiple strains
Chromosome	15
Molecular Note	This spontaneously arising frameshift deletion is located in exon 2 at position 103308576 bp (NCBI Build 37) and results in a premature stop codon. The mutation is predicted to result in a truncated protein lacking the transmembrane domains 4-7. The inbred strains BDP/J, DBA/1J, DBA/2J, I/LnJ, FVB/NJ, LG/J, MRL/MpJ, NODShi/LtJ, NOR/LtJ, P/J, PL/J, SKHIN/Sprd, SJL/J, SM/J are homozygous for the deletion. The allele is segregating in the outbred stocks ICR and CD-1.

Il3ra^m1

Allele Symbol: Il3ra^m1

Allele Name	mutation 1
Allele Type	Spontaneous
Allele Synonym(s)	Il3ra^A/J; Il3raⁿ
Gene Symbol and Name	Il3ra, interleukin 3 receptor, alpha chain
Gene Synonym(s)	CD123; Cyrl; IL-3 receptor alpha chain; IL3R; IL3RAY; IL3RX; IL3RY; SUT-1; hIL-3Ra
Strain of Origin	A/J
Chromosome	14
Molecular Note	Sequence analysis revealed A/J mice lack the sequence corresponding to exon 8, which encodes 10 amino acid residues in the extracellular domain. Aberrant splicing was due to a 5 base pair deletion at the branch point in intron 7.

Neu1^a

Allele Symbol: Neu1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	Neu-1^s
Gene Symbol and Name	Neu1, neuraminidase 1
Gene Synonym(s)	AA407268; AA407316; Aglp; Aglp; Apl; Apl; Bat-7; Bat-7; Bat7; Bat7; G9; HLA-B-associated transcript 7; Map-2; Map-2; NANH; NEU; Neu-1; Neu-1; SIAL1; acid phosphatase, liver; alpha glucosidase processing; expressed sequence AA407268; expressed sequence AA407316; lysosomal sialidase; mannosidase processing 2; sialidase 1
Strain of Origin	SM/J
Chromosome	17
General Note	Low activity determined by the Neu1^a allele occurs in the SM/J inbred strain and in wild mice in the area of Ann Arbor, Michigan; all other inbred strains have high activity determined by the Neu1^b allele. Heterozygotes have intermediate activity. Neuraminidase removes extra sialic acid residues from these enzymes. The defective neuraminidase of Neu1^a> mice, by failing to remove the extra sialic acid, changes their electrophoretic mobility (J:6480). Level of activity of neuraminidase in activated T lymphocytes is also depressed in Neu1^a/Neu1^a mice (J:7976).
Molecular Note	Sequencing of the a allele revealed 5 nucleotide differences compared to the b allele. These changes alter the amino acid residues 11, 15, 17, 19 and 209 of the encoded protein from Gly, Tyr, Ala, Arg and Leu to Arg, Cys, Val, Cys and Ile, respectively. This encoded protein has 85-95% less activity than the protein encoded by the b allele as demonstrated in an in vitro assay. Further studies attributed most of the activity loss to the variation at position 209. Later studies detected 4 silent mutations in the signal peptide sequence and 2 mutations (c.-240C>T and c.-519G>A) in the promoter region. The mutation c.-519G>A creates a novel binding site for Nkx3-1 and Nkx3-2. In vitro assays demonstrated that binding of Nkx3-2 specifically represses promoter driven expression.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cardiovascular Research
- Diet-Induced Atherosclerosis
  - Susceptible
Developmental Biology Research
- Growth Defects
Diabetes and Obesity Research
- Obesity Without Diabetes
  - diet-induced
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - defects in humoral immune responses

Genotype: Il3ra^m1 related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin

Mammalian Phenotype Terms by Genotype

Genotype: Il3ra^m1/Il3ra^m1
SM/J

hematopoietic system phenotype

abnormal common myeloid progenitor cell morphology
- CFU-GM assays using bone marrow derived cells yield very few colonies in repsonse to interleukin 3

Genotype: Neu1^a/Neu1^a
SM/J

homeostasis/metabolism phenotype

abnormal enzyme/coenzyme activity
- deficient enzymatic activity of neuraminidase was reported
- tissue specific decrease in sialidase activity

growth/size/body region phenotype

decreased body weight
- compared to C57BL/6 controls

liver/biliary system phenotype

abnormal Kupffer cell morphology
- cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls

renal/urinary system phenotype

abnormal proximal convoluted tubule morphology
- cells with high levels of membranous accumulations degenerate releasing cellular debris into the intercellular space and the lumen of the tubules
- some epithelial cells accumulate large membrane-bound structures containing membranous whorls

hematopoietic system phenotype

abnormal Kupffer cell morphology
- cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls

immune system phenotype

abnormal Kupffer cell morphology
- cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls

cardiovascular system phenotype

abnormal Kupffer cell morphology
- cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls

Phenotype Information

Festing Inbred Strain Characteristics: SM

References

Anunciado RV; Nishimura M; Mori M; Ishikawa A; Tanaka S; Horio F; Ohno T; Namikawa T. 2001. Quantitative trait loci for body weight in the intercross between SM/J and A/J mice. Exp Anim 50(4):319-24. PubMed: 11515095 MGI: J:71465
Ehrich TH; Kenney JP; Vaughn TT; Pletscher LS; Cheverud JM. 2003. Diet, obesity, and hyperglycemia in LG/J and SM/J mice. Obes Res 11(11):1400-10. PubMed: 14627762 MGI: J:86873
Korstanje R; Albers JJ; Wolfbauer G; Li R; Tu AY; Churchill GA; Paigen BJ. 2004. Quantitative trait locus mapping of genes that regulate phospholipid transfer activity in SM/J and NZB/BlNJ inbred mice. Arterioscler Thromb Vasc Biol 24(1):155-60. PubMed: 14592843 MGI: J:86340
Korstanje R; Li R; Howard T; Kelmenson P; Marshall J; Paigen B; Churchill G. 2004. Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population. J Lipid Res 45(5):881-8. PubMed: 14993241 MGI: J:89309
Macarthur JW. 1949. Selection for Small and Large Body Size in the House Mouse. Genetics 34(2):194-209. PubMed: 17247310 MGI: J:2452
Macarthur JW. 1944. Genetics of body size and related characters Am Naturalist 78:142-57. MGI: J:25351
Paigen B; Ishida BY; Verstuyft J; Winters RB; Albee D. 1990. Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice. Arteriosclerosis 10(2):316-23. PubMed: 2317166 MGI: J:22615
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11. PubMed: 15081119 MGI: J:89298
Pitman WA; Korstanje R; Churchill GA; Nicodeme E; Albers JJ; Cheung MC; Staton MA; Sampson SS; Harris S; Paigen B. 2002. Quantitative trait locus mapping of genes that regulate HDL cholesterol in SM/J and NZB/B1NJ inbred mice. Physiol Genomics 9(2):93-102. PubMed: 12006675 MGI: J:76707

Additional References

Anunciado RV; Nishimura M; Mori M; Ishikawa A; Tanaka S; Horio F; Ohno T; Namikawa T. 2003. Quantitative trait locus analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels in the (SM/J x A/J)F2 mice. Exp Anim 52(1):37-42. PubMed: 12638235 MGI: J:82274
Frankel WN; Lee BK; Stoye JP; Coffin JM; Eicher EM. 1992. Characterization of the endogenous nonecotropic murine leukemia viruses of NZB/B1NJ and SM/J inbred strains. Mamm Genome 2(2):110-22. PubMed: 1311971 MGI: J:1883
Hara T; Ichihara M; Takagi M; Miyajima A. 1995. Interleukin-3 (IL-3) poor-responsive inbred mouse strains carry the identical deletion of a branch point in the IL-3 receptor alpha subunit gene. Blood 85(9):2331-6. PubMed: 7727767 MGI: J:24918
Klingenberg CP; Leamy LJ; Cheverud JM. 2004. Integration and modularity of quantitative trait locus effects on geometric shape in the mouse mandible. Genetics 166(4):1909-21. PubMed: 15126408 MGI: J:89613
Rottier RJ; Bonten E; d'Azzo A. 1998. A point mutation in the neu-1 locus causes the neuraminidase defect in the SM/J mouse. Hum Mol Genet 7(2):313-21. PubMed: 9425240 MGI: J:77236
Routman EJ; Cheverud JM. 1995. Polymorphism for PCR-analyzed microsatellites between the inbred mouse strains LG and SM. Mamm Genome 6(6):401-4. PubMed: 7647461 MGI: J:26136

Additional - Gpr84^del related

Perez CJ; Dumas A; Vallieres L; Guenet JL; Benavides F. 2013. Several classical mouse inbred strains, including DBA/2, NOD/Lt, FVB/N, and SJL/J, carry a putative loss-of-function allele of Gpr84. J Hered 104(4):565-71. PubMed: 23616478 MGI: J:237405

Additional - Il3ra^m1 related

Gainsford T; Roberts AW; Kimura S; Metcalf D; Dranoff G; Mulligan RC; Begley CG; Robb L; Alexander WS. 1998. Cytokine production and function in c-mpl-deficient mice: no physiologic role for interleukin-3 in residual megakaryocyte and platelet production. Blood 91(8):2745-52. PubMed: 9531584 MGI: J:47462
Hara T; Ichihara M; Takagi M; Miyajima A. 1995. Interleukin-3 (IL-3) poor-responsive inbred mouse strains carry the identical deletion of a branch point in the IL-3 receptor alpha subunit gene. Blood 85(9):2331-6. PubMed: 7727767 MGI: J:24918
Ichihara M; Hara T; Takagi M; Cho LC; Gorman DM; Miyajima A. 1995. Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene. EMBO J 14(5):939-50. PubMed: 7889941 MGI: J:23971

Additional - Neu1^a related

Carrillo MB; Milner CM; Ball ST; Snoek M; Campbell RD. 1997. Cloning and characterization of a sialidase from the murine histocompatibility-2 complex: low levels of mRNA and a single amino acid mutation are responsible for reduced sialidase activity in mice carrying the Neu1a allele. Glycobiology 7(7):975-86. PubMed: 9363440 MGI: J:43930
Champigny MJ; Mitchell M; Fox-Robichaud A; Trigatti BL; Igdoura SA. 2009. A point mutation in the neu1 promoter recruits an ectopic repressor, Nkx3.2 and results in a mouse model of sialidase deficiency. Mol Genet Metab 97(1):43-52. PubMed: 19217813 MGI: J:147881
Hildebrand CE; Gonzalez FJ; Kozak CA; Nebert DW. 1985. Regional linkage analysis of the dioxin-inducible P-450 gene family on mouse chromosome 9. Biochem Biophys Res Commun 130(1):396-406. PubMed: 4040754 MGI: J:7969
Kijimoto-Ochiai S; Koda T; Suwama T; Matsukawa H; Fujii M; Tomobe K; Nishimura M. 2008. Low expression of Neu2 sialidase in the thymus of SM/J mice-existence of neuraminidase positive cells 'Neu-medullocyte' in the murine thymus. Glycoconj J 25(8):787-96. PubMed: 18553168 MGI: J:153934
Klein D; Klein J. 1982. Polymorphism of the Apl (Neu-1) locus in the mouse. Immunogenetics 16(2):181-4. PubMed: 7141491 MGI: J:6900
Potier M; Lu Shun Yan D; Womack JE. 1979. Neuraminidase deficiency in the mouse. FEBS Lett 108(2):345-8. PubMed: 520573 MGI: J:123679
Rottier RJ; Bonten E; d'Azzo A. 1998. A point mutation in the neu-1 locus causes the neuraminidase defect in the SM/J mouse. Hum Mol Genet 7(2):313-21. PubMed: 9425240 MGI: J:77236
Womack JE; David CS. 1982. Mouse gene for neuraminidase activity (Neu-1) maps to the D end of H-2. Immunogenetics 16(2):177-80. PubMed: 7141490 MGI: J:6899
Womack JE; Yan DL; Potier M. 1981. Gene for neuraminidase activity on mouse chromosome 17 near h-2: pleiotropic effects on multiple hydrolases. Science 212(4490):63-5. PubMed: 7209520 MGI: J:6480
Yang A; Gyulay G; Mitchell M; White E; Trigatti BL; Igdoura SA. 2012. Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice. J Lipid Res 53(12):2573-85. PubMed: 22984145 MGI: J:190648

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Also Known As: small

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

Gpr84del

Allele Symbol: Gpr84del

Il3ram1

Allele Symbol: Il3ram1

Neu1a

Allele Symbol: Neu1a

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Il3ram1 related

Mammalian Phenotype Terms by Genotype

Genotype: Il3ram1/Il3ram1SM/J

hematopoietic system phenotype

Genotype: Neu1a/Neu1aSM/J

homeostasis/metabolism phenotype

growth/size/body region phenotype

liver/biliary system phenotype

renal/urinary system phenotype

hematopoietic system phenotype

immune system phenotype

cardiovascular system phenotype

Phenotype Information

References

Additional References

Additional - Gpr84del related

Additional - Il3ram1 related

Additional - Neu1a related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Appearance

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gpr84^del

Allele Symbol: Gpr84^del

Il3ra^m1

Allele Symbol: Il3ra^m1

Neu1^a

Allele Symbol: Neu1^a

Genotype: Il3ra^m1 related

Genotype: Il3ra^m1/Il3ra^m1
SM/J

Genotype: Neu1^a/Neu1^a
SM/J

Additional - Gpr84^del related

Additional - Il3ra^m1 related

Additional - Neu1^a related