Overview

Also Known As: C3Sn, C3H Snell, C3H/HeDiSnJ

C3H mice are used as a general purpose strain in a wide variety of research areas including cancer, infectious disease, sensorineural, and cardiovascular biology research. This C3H substrain was rederived in the 1960's and again in 1999, as a result, this line does not carry the exogenous mouse mammary tumor virus (MMTV). These mice are homozygous for Clcc1^m1J, which causes increased sensitivity to endoplasmic reticulum stress in the cerebellum, and homozygous for the retinal degeneration allele Pde6b^rd1.

Genetic overview

Genetic Background	Generation
	F191+F32 (2018-04-02 00:00:00)

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Research Applications

Neurobiology Research
Sensorineural Research
Mouse/Human Gene Homologs
Research Tools

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Base Price

Starting at:

$231.00 Domestic price for female

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Details

Important Note

This strain does not carry mouse mammary tumor virus (MMTV). See JAX^® NOTES, May 2000, No. 480. This strain is also homozygous for the retinal degeneration allele Pde6b^rd1. A sighted alternative is Stock No. 003648, C3Sn.BLiA-Pde6b⁺/DnJ.

Detailed Description

C3H mice are used as a general purpose strain in a wide variety of research areas including cancer, infectious disease, sensorineural, and cardiovascular biology research. The C3H/HeSnJ substrain served as the parental strain for the Snell (Sn) congenic strains. This substrain does not carry the exogenous mouse mammary tumor virus (MMTV) and, like the other C3H strains, is homozygous for the retinal degeneration allele (rd1), but lacks the nob5 allele of Gpr179 (Chang, 2015). Unlike other C3H inbred strains, this strain is homozygous for the Clcc1^m1J allele, which has a more mild phenotype on this background than on the C57BL/6J congenic background (see Stock# 007723). C3H/HeSnJ have pyknotic granule cells in the cerebellum by 2 months of age, and hypoplastic cerebellum with numerous vacuoles by 22 months of age (Jia et al, 2015).

Development

The C3H parent strain was developed by LC Strong in 1920 from a cross of a Bagg albino female with a DBA male followed by selection for high incidence of mammary tumors. This high incidence resulted from exogenous mouse mammary tumor virus (MMTV) transmitted through the mother's milk. The Jackson Laboratory maintains four C3H substrains, C3H/HeJ (Stock No. 000659), C3H/HeOuJ (Stock No. 000635), C3HeB/FeJ (Stock No. 000658) and C3H/HeSnJ (Stock No. 000661) that are now free of exogenous MMTV. C3H/HeSnJ, C3H/HeJ and C3H/HeOuJ mice previously carried MMTV but were rederived in 1999 during planned efforts to increase the overall health status of the mice and the virus was not reintroduced. A spontaneous mutation occurred in C3H/HeJ sometime between 1960 and 1968 at lipopolysaccharide response locus (mutation in toll-like receptor 4 gene, Tlr4^lps) making C3H/HeJ mice endotoxin resistant while the other three C3H strains are endotoxin sensitive.

Selected References

Jia Y; Jucius TJ; Cook SA; Ackerman SL. 2015. Loss of Clcc1 Results in ER Stress, Misfolded Protein Accumulation, and Neurodegeneration. J Neurosci 35(7):3001-9PubMed: 25698737 MGI: J:218443
Chang B. 2015. Survey of the nob5 mutation in C3H substrains. Mol Vis 21:1101-5PubMed: 26396487 MGI: J:224435
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298

View All References

Genetics

Clcc1^m1J

Allele Symbol: Clcc1^m1J

Allele Name	mutation 1, Jackson
Allele Type	Spontaneous
Allele Synonym(s)	mutation 1, Jackson; Clcc1^m1J
Gene Symbol and Name	Clcc1, chloride channel CLIC-like 1
Gene Synonym(s)	Mclc; Mid-1-related chloride channel 1; MCLC; Mclc
Strain of Origin	C3H/HeSnJ
Chromosome	3
Molecular Note	The mutation is an intracisternal A-particle (IAP) retrotransposon insertion into a C3H/HeSnJ genomic region. The IAP is inserted into intron 2 of the gene and disrupts the normal splicing of the mRNA transcribed from this gene. Resulting in-frame stop codons from the insertion of IAP sequences resulted in severely reduced protein levels in mutant tissues. The retrotransposition of this IAP is a recent event occurring during, or after, the establishment of the C3H/HeSnJ substrain.

Pde6b^rd1

Allele Symbol: Pde6b^rd1

Allele Name	retinal degeneration 1
Allele Type	Spontaneous
Allele Synonym(s)	retinal degeneration 1; Pde6b^rd1
Gene Symbol and Name	Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Gene Synonym(s)	rd; rd1; Pdeb; r; rd; rd10; nmf137; r; retinal degeneration; retinal degeneration 1; retinal degeneration 10; CSNB3; CSNBAD2; rd1; rd-1; rd10; PDEB; RP40; phosphodiesterase, cGMP, rod receptor, beta polypeptide; Pdeb; GMP-PDEbeta
Strain of Origin	various
Chromosome	5
General Note	The following inbred strains are known to be homozygous for Pde6b: C3H sublines, CBA/J, FVB/NJ, PL/J, SB, SJL/J, and SWR/J.
Molecular Note	Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C to A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain has also been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested.

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

retinitis pigmentosa 40

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Clcc1^m1J related

Neurobiology Research
- Ataxia (Movement) Defects
- Cerebellar Defects
- Neurodegeneration

Genotype: Pde6b^rd1 related

Sensorineural Research
- Retinal Degeneration
Mouse/Human Gene Homologs
- retinitis pigmentosa, autosomal recessive

Research Tools
- General Purpose
Sensorineural Research
- Retinal Degeneration
  - Homozygous for Pde6b^rd1
- Hearing Defects
  - Age related hearing loss, control
Neurobiology Research
- Hearing Defects
  - Age related hearing loss, control

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Clcc1^m1J/Clcc1^m1J
C3H/HeSnJ

mammalian phenotype

nervous system phenotype
- Background Sensitivity - The peripheral neuropathy, neurogenic muscular atrophy and mild truncal ataxia that is found on the C57BL/6J background is not found on the C3H/HeSnJ background

nervous system phenotype

abnormal cerebellar granule cell morphology
- Background Sensitivity - pyknotic granule cells are found in the cerebellum as early as 2 months of age, although cell death progresses more slowly than in homozygotes on the C57BL/6J congenic background

brain vacuoles
- Background Sensitivity - found by 22 months of age, later onset and decreased severity than that found on the C57BL/6J background

cerebellum hypoplasia
- Background Sensitivity - cerebellum is hypoplastic by 22 months of age compared with that of C3H/HeOuJ, C3HeB/FeJ and C3H/HeJ inbred mice, which lack this IAP

The following phenotype relates to a compound genotype created using this strain

Genotype: Pde6b^rd1/Pde6b^rd1
C3H/HeJ

mammalian phenotype

vision/eye phenotype
- Normal - despite the absence of rods, mice exhibit normal photopotentiation (defined as a 50% augmentation in pupillary light response (PLR) compared to pre-bright light PLR during a one minute dim blue light exposure after bright light exposure)

nervous system phenotype

absent photoreceptor outer segment

vision/eye phenotype

abnormal retinal outer nuclear layer morphology
- nearly complete absence of outer nuclear layer

absent photoreceptor outer segment

retinal degeneration
- entire outer retina is destroyed, however the inner retina remains intact

Phenotype Information

Festing Inbred Strain Characteristics: C3H

References

Jia Y; Jucius TJ; Cook SA; Ackerman SL. 2015. Loss of Clcc1 Results in ER Stress, Misfolded Protein Accumulation, and Neurodegeneration. J Neurosci 35(7):3001-9PubMed: 25698737 MGI: J:218443
Chang B. 2015. Survey of the nob5 mutation in C3H substrains. Mol Vis 21:1101-5PubMed: 26396487 MGI: J:224435
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298

Additional References

International Nomenclature Committee. 1952. COMMITTEE on Standardized Nomenclature for Inbred Strains of Mice Cancer Res 12(8):602-13PubMed: 14945054 MGI: J:166288
Zheng QY; Johnson KR; Erway LC. 1999. Assessment of hearing in 80 inbred strains of mice by ABR threshold analyses. Hear Res 130(1-2):94-107PubMed: 10320101 MGI: J:54812

Additional - Clcc1^m1J related

Additional - Pde6b^rd1 related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR: Clcc1^m1J
- Genotyping resources and troubleshooting
Mating System
- Sibling x Sibling
Appearance
- agouti
  Related Genotype: A/A
Citation
When using the C3H/HeSnJ mouse strain in a publication, please include JAX stock #000661 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

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Live Mouse

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Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Inbred, 1 pair minimum will be supplied

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: C3Sn, C3H Snell, C3H/HeDiSnJ

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Selected References

Clcc1m1J

Allele Symbol: Clcc1m1J

Pde6brd1

Allele Symbol: Pde6brd1

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Clcc1m1J related

Genotype: Pde6brd1 related

Mammalian Phenotype Terms by Genotype

Genotype: Clcc1m1J/Clcc1m1JC3H/HeSnJ

mammalian phenotype

nervous system phenotype

Genotype: Pde6brd1/Pde6brd1C3H/HeJ

mammalian phenotype

nervous system phenotype

vision/eye phenotype

Phenotype Information

References

Additional References

Additional - Clcc1m1J related

Additional - Pde6brd1 related

Genotyping Protocols

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Clcc1^m1J

Allele Symbol: Clcc1^m1J

Pde6b^rd1

Allele Symbol: Pde6b^rd1

Genotype: Clcc1^m1J related

Genotype: Pde6b^rd1 related

Genotype: Clcc1^m1J/Clcc1^m1J
C3H/HeSnJ

Genotype: Pde6b^rd1/Pde6b^rd1
C3H/HeJ

Additional - Clcc1^m1J related

Additional - Pde6b^rd1 related