Overview

Also Known As:C3Sn, C3H Snell, C3H/HeDiSnJ

C3H mice are used as a general purpose strain in a wide variety of research areas including cancer, infectious disease, sensorineural, and cardiovascular biology research. This C3H substrain was rederived in the 1960's and again in 1999, as a result, this line does not carry the exogenous mouse mammary tumor virus (MMTV). These mice are homozygous for Clcc1^m1J, which causes increased sensitivity to endoplasmic reticulum stress in the cerebellum, and homozygous for the retinal degeneration allele Pde6b^rd1.

Genetic overview

Genetic Background	Generation
	F163+pF12 (2021-04-05 00:00:00)

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Research Applications

Neurobiology Research
Sensorineural Research
Mouse/Human Gene Homologs
Research Tools

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Base Price

Starting at:

$236.78 Domestic price for female 4-week

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Details

Important Note

This strain does not carry mouse mammary tumor virus (MMTV). See JAX^® NOTES, May 2000, No. 480. This strain is also homozygous for the retinal degeneration allele Pde6b^rd1. A sighted alternative is Stock No. 003648, C3Sn.BLiA-Pde6b⁺/DnJ.

Detailed Description

C3H mice are used as a general purpose strain in a wide variety of research areas including cancer, infectious disease, sensorineural, and cardiovascular biology research. The C3H/HeSnJ substrain served as the parental strain for the Snell (Sn) congenic strains. This substrain does not carry the exogenous mouse mammary tumor virus (MMTV) and, like the other C3H strains, is homozygous for the retinal degeneration allele (rd1), but lacks the nob5 allele of Gpr179 (Chang, 2015). Unlike other C3H inbred strains, this strain is homozygous for the Clcc1^m1J allele, which has a more mild phenotype on this background than on the C57BL/6J congenic background (see Stock# 007723). C3H/HeSnJ have pyknotic granule cells in the cerebellum by 2 months of age, and hypoplastic cerebellum with numerous vacuoles by 22 months of age (Jia et al, 2015).

Development

The C3H parent strain was developed by LC Strong in 1920 from a cross of a Bagg albino female with a DBA male followed by selection for high incidence of mammary tumors (LC Strong, 1935). This high incidence resulted from exogenous mouse mammary tumor virus (MMTV) transmitted through the mother's milk. The Jackson Laboratory maintains four C3H substrains, C3H/HeJ (Stock No. 000659), C3H/HeOuJ (Stock No. 000635), C3HeB/FeJ (Stock No. 000658) and C3H/HeSnJ (Stock No. 000661) that are now free of exogenous MMTV. C3H/HeSnJ, C3H/HeJ and C3H/HeOuJ mice previously carried MMTV but were rederived in 1999 during planned efforts to increase the overall health status of the mice and the virus was not reintroduced. A spontaneous mutation occurred in C3H/HeJ sometime between 1960 and 1968 at lipopolysaccharide response locus (mutation in toll-like receptor 4 gene, Tlr4^lps) making C3H/HeJ mice endotoxin resistant while the other three C3H strains are endotoxin sensitive.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Chang B. 2015. Survey of the nob5 mutation in C3H substrains. Mol Vis 21:1101-5PubMed: 26396487 MGI: J:224435
Jia Y; Jucius TJ; Cook SA; Ackerman SL. 2015. Loss of Clcc1 Results in ER Stress, Misfolded Protein Accumulation, and Neurodegeneration. J Neurosci 35(7):3001-9PubMed: 25698737 MGI: J:218443
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Strong LC. 1935. The Establishment of the C(3)H Inbred Strain of Mice for the Study of Spontaneous Carcinoma of the Mammary Gland. Genetics 20(6):586-91PubMed: 17246779 MGI: J:2480

View All References

Genetics

Pde6b^rd1

Allele Symbol: Pde6b^rd1

Allele Name	retinal degeneration 1
Allele Type	Spontaneous
Allele Synonym(s)	Pdeb^rd1; rd; rd1; rd-1; rodless retina
Gene Symbol and Name	Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Gene Synonym(s)
Strain of Origin	various
Chromosome	5
General Note	The following inbred strains are known to be homozygous for Pde6b: C3H sublines, CBA/J, FVB/NJ, PL/J, SB, SJL/J, and SWR/J.
Molecular Note	Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C-to-A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain, has been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested.

Clcc1^m1J

Allele Symbol: Clcc1^m1J

Allele Name	mutation 1, Jackson
Allele Type	Spontaneous
Allele Synonym(s)	nm2453; sprawler
Gene Symbol and Name	Clcc1, chloride channel CLIC-like 1
Gene Synonym(s)
Strain of Origin	C3H/HeSnJ
Chromosome	3
Molecular Note	The mutation is an intracisternal A-particle (IAP) retrotransposon insertion into a C3H/HeSnJ genomic region. The IAP is inserted into intron 2 of the gene and disrupts the normal splicing of the mRNA transcribed from this gene. Resulting in-frame stop codons from the insertion of IAP sequences resulted in severely reduced protein levels in mutant tissues. The retrotransposition of this IAP is a recent event occurring during, or after, the establishment of the C3H/HeSn substrain.

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

retinitis pigmentosa 40

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Clcc1^m1J related

Neurobiology Research
- Ataxia (Movement) Defects
- Cerebellar Defects
- Neurodegeneration

Genotype: Pde6b^rd1 related

Sensorineural Research
- Retinal Degeneration
Mouse/Human Gene Homologs
- retinitis pigmentosa, autosomal recessive

Research Tools
- General Purpose
Sensorineural Research
- Retinal Degeneration
  - Homozygous for Pde6b^rd1
- Hearing Defects
  - Age related hearing loss, control
Neurobiology Research
- Hearing Defects
  - Age related hearing loss, control

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Clcc1^m1J/Clcc1^m1J
C3H/HeSnJ

mammalian phenotype

nervous system phenotype
- Background Sensitivity - The peripheral neuropathy, neurogenic muscular atrophy and mild truncal ataxia that is found on the C57BL/6J background is not found on the C3H/HeSnJ background

nervous system phenotype

abnormal cerebellar granule cell morphology
- Background Sensitivity - pyknotic granule cells are found in the cerebellum as early as 2 months of age, although cell death progresses more slowly than in homozygotes on the C57BL/6J congenic background

brain vacuoles
- Background Sensitivity - found by 22 months of age, later onset and decreased severity than that found on the C57BL/6J background

cerebellum hypoplasia
- Background Sensitivity - cerebellum is hypoplastic by 22 months of age compared with that of C3H/HeOuJ, C3HeB/FeJ and C3H/HeJ inbred mice, which lack this IAP

The following phenotype relates to a compound genotype created using this strain

Genotype: Pde6b^rd1/Pde6b^rd1
C3H/HeJ

mammalian phenotype

vision/eye phenotype
- Normal - despite the absence of rods, mice exhibit normal photopotentiation (defined as a 50% augmentation in pupillary light response (PLR) compared to pre-bright light PLR during a one minute dim blue light exposure after bright light exposure)

nervous system phenotype

absent photoreceptor outer segment

vision/eye phenotype

abnormal retinal outer nuclear layer morphology
- nearly complete absence of outer nuclear layer

retinal degeneration
- entire outer retina is destroyed, however the inner retina remains intact

absent photoreceptor outer segment

Phenotype Information

Festing Inbred Strain Characteristics: C3H

References

Chang B. 2015. Survey of the nob5 mutation in C3H substrains. Mol Vis 21:1101-5PubMed: 26396487 MGI: J:224435
Jia Y; Jucius TJ; Cook SA; Ackerman SL. 2015. Loss of Clcc1 Results in ER Stress, Misfolded Protein Accumulation, and Neurodegeneration. J Neurosci 35(7):3001-9PubMed: 25698737 MGI: J:218443
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Strong LC. 1935. The Establishment of the C(3)H Inbred Strain of Mice for the Study of Spontaneous Carcinoma of the Mammary Gland. Genetics 20(6):586-91PubMed: 17246779 MGI: J:2480

Additional References

International Nomenclature Committee. 1952. COMMITTEE on Standardized Nomenclature for Inbred Strains of Mice Cancer Res 12(8):602-13PubMed: 14945054 MGI: J:166288
Zheng QY; Johnson KR; Erway LC. 1999. Assessment of hearing in 80 inbred strains of mice by ABR threshold analyses. Hear Res 130(1-2):94-107PubMed: 10320101 MGI: J:54812

Additional - Clcc1^m1J related

Additional - Pde6b^rd1 related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Clcc1
- Probe:Clcc1 Probe
- Genotyping resources and troubleshooting
Mating System
- Sibling x Sibling
Appearance
- agouti
  Related Genotype: A/A
Citation
When using the C3H/HeSnJ mouse strain in a publication, please include JAX stock #000661 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX27 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	inbred

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:C3Sn, C3H Snell, C3H/HeDiSnJ

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Pde6brd1

Allele Symbol: Pde6brd1

Clcc1m1J

Allele Symbol: Clcc1m1J

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Clcc1m1J related

Genotype: Pde6brd1 related

Mammalian Phenotype Terms by Genotype

Genotype: Clcc1m1J/Clcc1m1JC3H/HeSnJ

mammalian phenotype

nervous system phenotype

Genotype: Pde6brd1/Pde6brd1C3H/HeJ

mammalian phenotype

nervous system phenotype

vision/eye phenotype

Phenotype Information

References

Additional References

Additional - Clcc1m1J related

Additional - Pde6brd1 related

Genotyping Protocols

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Pde6b^rd1

Allele Symbol: Pde6b^rd1

Clcc1^m1J

Allele Symbol: Clcc1^m1J

Genotype: Clcc1^m1J related

Genotype: Pde6b^rd1 related

Genotype: Clcc1^m1J/Clcc1^m1J
C3H/HeSnJ

Genotype: Pde6b^rd1/Pde6b^rd1
C3H/HeJ

Additional - Clcc1^m1J related

Additional - Pde6b^rd1 related