Overview

Also Known As:A Woolley Snell

A/WySnJ mice are similar to A/J mice (#000646), but carry a spontaneous mutation in Tnfrsf13c and exhibit a significant loss of mature B cells.

Genetic overview

Genetic Background	Generation

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Research Applications

Developmental Biology Research
Immunology, Inflammation and Autoimmunity Research
Cancer Research
Neurobiology Research
Research Tools
Sensorineural Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

This strain is homozygous for Cdh23^ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss with onset after 10 months of age.

Detailed Description

The A inbred strain, from which this substrain is derived, is used widely used in cancer and immunology research. It is highly susceptible to induction of congenital cleft palate by cortisone. It has a high incidence of spontaneous lung adenomas and lung tumors readily develop in response to carcinogens. High percentage of mammary adenocarcinomas (a large proportion acinar type) develop in multiparous females. Rare spontaneous myoepitheliomas arising from myoepithelial cells of various exocrine glands have been observed in The Jackson Laboratory substrains.

Unlike A/J mice, A/WySnJ mice carry a spontaneous mutation in Tnfrsf13c and exhibit a significant loss of mature B cells (Miller, et al., 1991, Lentz et al., 1996, Shulga-Morskaya et al., 2004).

Development

This strain is a substrain of the A strain that was developed by LC Strong in 1921 from a cross between a Cold Spring Harbor albino and a Bagg albino. The substrain branch leading to A/WySnJ has been separated from the original A strain and the branch leading to the A/J substrain since about 1930.

Genetics

Tnfrsf13c^Bcmd1

Allele Symbol: Tnfrsf13c^Bcmd1

Allele Name	B-cell maturation defect 1
Allele Type	Spontaneous
Allele Synonym(s)	Baffr^m; Bcmd1^A/WySnJ; Tnfrsf13c^{Bcmd1-A/WySnJ}
Gene Symbol and Name	Tnfrsf13c, tumor necrosis factor receptor superfamily, member 13c
Gene Synonym(s)
Strain of Origin	A/WySnJ
Chromosome	15
Molecular Note	In the A/WySnJ strain of mice, an intracisternal type A particle retrotransposon insertion occurred in exon 3, resulting in the replacememt of the final 8 amino acids of the encoded protein with 21 amino acids from the inserted element. This mutation is not present in A/J. FACS analysis indicated that an aberrant fusion protein was expressed from this allele that appeared on the cell surface; however, the protein appeared to be nonfuctional.

Il3ra^m1

Allele Symbol: Il3ra^m1

Allele Name	mutation 1
Allele Type	Spontaneous
Allele Synonym(s)	Il3ra^A/J; Il3raⁿ
Gene Symbol and Name	Il3ra, interleukin 3 receptor, alpha chain
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	14
General Note	This allele has been identified in A/J, A/WySnJ, A/HeJ, C58/J, RF/J, AKR/J, SM/J, BUB/BnJ, CE/J, and NZB/B1NJ. see J:24918.
Molecular Note	Sequence analysis revealed A/J mice lack the sequence corresponding to exon 8, which encodes 10 amino acid residues in the extracellular domain. Aberrant splicing was due to a 5 base pair deletion at the branch point in intron 7.

Hc⁰

Allele Symbol: Hc⁰

Allele Name	deficient
Allele Type	Spontaneous
Allele Synonym(s)	C5-; C5-d; C5-def; C5-deficient; Hc^Hfib2; hc^o
Gene Symbol and Name	Hc, hemolytic complement
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	2
General Note	This is an allele characteristic of various inbred mouse strains including the following: A/HeJ, A/J, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The A/J allele contains a 2 bp deletion resulting in deficient Hc mRNA and protein production and is associated with susceptibility to allergen-induced bronchial hyperresponsiveness. (J:108211)
Molecular Note	A 2 base "TA" deletion at positions 62 and 63 of an 83 base pair exon near the 5' end of the gene is found in the following mouse strains: A/HeJ, A/J, AKR/J, DBA/2J, I/LnJ, KK/HlJ, MOLF/EiJ, NZB/B1NJ, RF/J, ST/bJ SWR/J, B10.D2/oSnJ. The consequence of this deletion is the creation of a stop codon starting four bases after the deletion. A truncated product of 216 amino acids is predicted as a result although contradictory reports exist that a larger pro-C5 protein may be synthesized. Nevertheless, macrophages from mouse strains carrying this allele do not secrete complement 5.

Wnt9b^clf1

Allele Symbol: Wnt9b^clf1

Allele Name	cleft lip 1
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Wnt9b, wingless-type MMTV integration site family, member 9B
Gene Synonym(s)
Strain of Origin	A/WySn
Chromosome	11
General Note	Unequal duplicate epistasis - the normal allele at clf1 is a dominant suppressor of the recessive phenotype at clf2, and the normal allele at clf2 is a semidominant suppressor of the recessive phenotype at the clf1 locus.
Molecular Note	This mutation is a novel insertion of an IAP transposon 3' from the gene. In addition, a standard genetic test of allelism between clf1 and a Wnt9b targeted mutation demonstrated noncomplementation, showing clf1 is an allele of Wnt9b.

Cdh23^ahl

Allele Symbol: Cdh23^ahl

Allele Name	age related hearing loss 1
Allele Type	Spontaneous
Allele Synonym(s)	Cdh23^753A; mdfw
Gene Symbol and Name	Cdh23, cadherin 23 (otocadherin)
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	10
Molecular Note	Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has shown this is caused by a G to A transition at coding nucleotide position 753 of Cdh23 (SNP rs257098870). This hypomorphic allele changes splice donor site G-GT to A-GT, causing frame skipping of exon 7. This is predicted to delete part of the 2nd and 3rd ectodomains and cause reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD-1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LtJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: 129S1/SvImJ, C3H/HeSnJ, I/LnJ, YBR/Ei, MRL/MpJ.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Craniofacial and Palate Defects
  - congenital cleft palate
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - specific complement deficiency
  - B cell deficiency
Cancer Research
- Increased Tumor Incidence
  - Adenomas: lung
  - Mammary Gland Tumors: late onset
  - Adenomas
  - Mammary Gland Tumors
Neurobiology Research
- Hearing Defects
  - Age related hearing loss
Sensorineural Research
- Hearing Defects
  - Age related hearing loss

Genotype: Hc⁰ related

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - specific complement deficiency, C5 complement
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - specific complement deficiency

Genotype: Il3ra^m1 related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin

Genotype: Cdh23^ahl related

Neurobiology Research
- Hearing Defects
  - Age related hearing loss
Sensorineural Research
- Hearing Defects
  - Age related hearing loss

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Tnfrsf13c^Bcmd1/Tnfrsf13c^Bcmd1
A/WySnJ

cellular phenotype

decreased B cell proliferation
- cultured B cells exhibited greatly reduced proliferation upon stimulation with LPS, anti-CD40, anti-IgM, and Il4

decreased T cell proliferation
- in vitro, fewer CD4+ cells enter the cell cycle and those that do undergo fewer rounds of division

hematopoietic system phenotype

abnormal lymphocyte physiology
- the mixed lymphocyte response is reduced 3- to 4-fold

decreased B cell proliferation
- cultured B cells exhibited greatly reduced proliferation upon stimulation with LPS, anti-CD40, anti-IgM, and Il4

decreased IgG level
- severe impairment of anti-NP IgG production

decreased T cell proliferation
- in vitro, fewer CD4+ cells enter the cell cycle and those that do undergo fewer rounds of division

immune system phenotype

abnormal lymphocyte physiology
- the mixed lymphocyte response is reduced 3- to 4-fold

decreased B cell proliferation
- cultured B cells exhibited greatly reduced proliferation upon stimulation with LPS, anti-CD40, anti-IgM, and Il4

decreased IgG level
- severe impairment of anti-NP IgG production

decreased interferon-gamma secretion
- less interferon-gamma is secreted in a mixed lymphocyte response

decreased T cell proliferation
- in vitro, fewer CD4+ cells enter the cell cycle and those that do undergo fewer rounds of division

increased length of allograft survival
- MHC-mismatched allograft retention is prolonged to 40 days with 50% of mice retaining allografts for greater than 100 days with the use of cyclosporine A compared to an enhancement of only a few days in wild-type mice
- mice retain MHC-mismatched allografts for 17 days compared to 1 week in wild-type mice

Genotype: Il3ra^m1/Il3ra^m1
A/WySnJ

hematopoietic system phenotype

abnormal common myeloid progenitor cell morphology
- CFU-GM assays using bone marrow derived cells yield very few colonies in repsonse to interleukin 3

Phenotype Information

Festing Inbred Strain Characteristics: A

References

Additional References

Amanna IJ; Clise-Dwyer K; Nashold FE; Hoag KA; Hayes CE. 2001. Cutting Edge: A/WySnJ Transitional B Cells Overexpress the Chromosome 15 Proapoptotic Blk Gene and Succumb to Premature Apoptosis. J Immunol 167(11):6069-72PubMed: 11714762 MGI: J:72848
Gong SG. 2001. Phenotypic and molecular analyses of A/WySn mice. Cleft Palate Craniofac J 38(5):486-91PubMed: 11522170 MGI: J:72150
Hara T; Ichihara M; Takagi M; Miyajima A. 1995. Interleukin-3 (IL-3) poor-responsive inbred mouse strains carry the identical deletion of a branch point in the IL-3 receptor alpha subunit gene. Blood 85(9):2331-6PubMed: 7727767 MGI: J:24918
Miller DJ; Hanson KD; Carman JA; Hayes CE. 1992. A single autosomal gene defect severely limits IgG but not IgM responses in B lymphocyte-deficient A/WySnJ mice. Eur J Immunol 22(2):373-9PubMed: 1531637 MGI: J:2761
Miller DJ; Hayes CE. 1991. Phenotypic and genetic characterization of a unique B lymphocyte deficiency in strain A/WySnJ mice. Eur J Immunol 21(5):1123-30PubMed: 2037009 MGI: J:25731
Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Shulga-Morskaya S; Dobles M; Walsh ME; Ng LG; MacKay F; Rao SP; Kalled SL; Scott ML. 2004. B cell-activating factor belonging to the TNF family acts through separate receptors to support B cell survival and T cell-independent antibody formation. J Immunol 173(4):2331-41PubMed: 15294946 MGI: J:92676
Sundberg JP; Hanson CA; Roop DR; Brown KS; Bedigian HG. 1991. Myoepitheliomas in inbred laboratory mice. Vet Pathol 28(4):313-23PubMed: 1719689 MGI: J:22767
Zheng QY; Johnson KR; Erway LC. 1999. Assessment of hearing in 80 inbred strains of mice by ABR threshold analyses. Hear Res 130(1-2):94-107PubMed: 10320101 MGI: J:54812
Zheng QY; Tong YC; Alagramam KN; Yu H. 2007. Tympanometry assessment of 61 inbred strains of mice. Hear Res 231(1-2):23-31PubMed: 17611057 MGI: J:123543

Additional - Cdh23^ahl related

Additional - Hc⁰ related

Additional - Il3ra^m1 related

Additional - Tnfrsf13c^Bcmd1 related

Additional - Wnt9b^clf1 related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- albino
  Related Genotype: a/a Tyrp1^b/Tyrp1^b Tyr^c/Tyr^c
Citation
When using the A Woolley Snell mouse strain in a publication, please include JAX stock #000647 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:A Woolley Snell

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Tnfrsf13cBcmd1

Allele Symbol: Tnfrsf13cBcmd1

Il3ram1

Allele Symbol: Il3ram1

Hc0

Allele Symbol: Hc0

Wnt9bclf1

Allele Symbol: Wnt9bclf1

Cdh23ahl

Allele Symbol: Cdh23ahl

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Hc0 related

Genotype: Il3ram1 related

Genotype: Cdh23ahl related

Mammalian Phenotype Terms by Genotype

Genotype: Tnfrsf13cBcmd1/Tnfrsf13cBcmd1A/WySnJ

cellular phenotype

hematopoietic system phenotype

immune system phenotype

Genotype: Il3ram1/Il3ram1A/WySnJ

hematopoietic system phenotype

Phenotype Information

References

Additional References

Additional - Cdh23ahl related

Additional - Hc0 related

Additional - Il3ram1 related

Additional - Tnfrsf13cBcmd1 related

Additional - Wnt9bclf1 related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Tnfrsf13c^Bcmd1

Allele Symbol: Tnfrsf13c^Bcmd1

Il3ra^m1

Allele Symbol: Il3ra^m1

Hc⁰

Allele Symbol: Hc⁰

Wnt9b^clf1

Allele Symbol: Wnt9b^clf1

Cdh23^ahl

Allele Symbol: Cdh23^ahl

Genotype: Hc⁰ related

Genotype: Il3ra^m1 related

Genotype: Cdh23^ahl related

Genotype: Tnfrsf13c^Bcmd1/Tnfrsf13c^Bcmd1
A/WySnJ

Genotype: Il3ra^m1/Il3ra^m1
A/WySnJ

Additional - Cdh23^ahl related

Additional - Hc⁰ related

Additional - Il3ra^m1 related

Additional - Tnfrsf13c^Bcmd1 related

Additional - Wnt9b^clf1 related