These mice carry a spontaneous mutation at the Sptbn4 locus characterized by locomotor instability, pronounced quivering, deafness, varying degrees of paralysis of the hindlegs, clasping of the hindlegs when held up by the tail, and priapism in most males.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Sptbn4 | spectrin beta, non-erythrocytic 4 |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | a | nonagouti |
Mice homozygous for the quivering-Jackson spontaneous mutation (Sptbn4qv-J) are characterized by locomotor instability, pronounced quivering, deafness, varying degrees of paralysis of the hindlegs, clasping of the hindlegs when held up by the tail, and priapism in most males. Serial sections of the brain, cord, and nerve roots reveal no abnormalities, and urinary amino acids are normal. The inner ear is histologically normal and has normal thresholds for compound action potentials at the round window. However, the thresholds for potentials in the inferior colliculus are twice as high as normal, showing that the deafness, unlike that of any other deaf mutants in the mouse, is of central origin. Viability of homozygous mutant mice at weaning is normal, but the life span is short, the majority dying before 5 months of age. Males are sterile, but females may be fertile and nurse their young.
The spontaneous mutation quivering Jackson (Sptbn4qv-J) arose in The Jackson Laboratory vibrating (Pitpnavb) strain when it was at generation F40 in 1974. The vibrating mutation arose in strain DBA/2J and was outcrossed once to C57BL/6J before inbreeding. The first affected Sptbn4qv-J female to breed was mated to a C3FeB6-A/Aw-J hybrid, and the stock was maintained by mating a quivering female to a C3FeB6-A/Aw male in the cross generation and sibling pairs in the intercross generation. It was cryopreserved in 1986 by mating heterozygous males to heterozygous females at N30.
Allele Name | quivering Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Sptbn4, spectrin beta, non-erythrocytic 4 |
Gene Synonym(s) | |
Strain of Origin | D2XB-Pitpnavb |
Chromosome | 7 |
Molecular Note | This spontaneous mutation was proven allelic with the original quivering mutation by a noncomplementation test. Sequencing of the Spnb4 gene only detected three silent third-base polymorphisms in the coding region and these polymorphisms can be identified in unaffected inbred strains. |
Allele Name | wild-type agouti |
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Allele Type | Spontaneous |
Allele Synonym(s) | dark-bellied agouti |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | various |
Chromosome | 2 |
General Note | The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back. |
Molecular Note | This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene. |
When using the quivering Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000638 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Homozygous for Spnb4<qv-J> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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