Overview

Mice of this stock carry the T(10;13)199H reciprocal translocation and either Lyst^bg-J or Lyst^bg-2J.

Genetic overview

Genetic Background	Generation

Lyst^bg-J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Lyst	lysosomal trafficking regulator

Lyst^bg-2J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Lyst	lysosomal trafficking regulator

a

Allele Type	Gene Symbol	Gene Name
Spontaneous	a	nonagouti

A

Allele Type	Gene Symbol	Gene Name
Spontaneous	a	nonagouti

Marker(s)

	Marker Symbol	Marker Name
	T(10;13)199H	reciprocal translocation, Chr 10 and 13, Harwell 199

View Genetics

Research Applications

Hematological Research
Internal/Organ Research
Mouse/Human Gene Homologs
Research Tools
Immunology, Inflammation and Autoimmunity Research
Cardiovascular Research
Dermatology Research
Metabolism Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Because males carrying the T(10;13)199H translocation are either sterile or die in utero, the translocation is propagated by mating females carrying the translocation to (C3H-Lyst^bg-2J x B6-Lyst^bg-J)F1 males. Agouti or non-agouti offspring will be carriers of the translocation and offspring having the beige coat color are non-carriers of the translocation.

Development

The translocation was induced by X-irradiation of a (C3H x 101)F1 male, and imported from Harwell via Mary Lyon. Embryos produced by mating B6C3 a/A-T(10;13)199H +/+ -Lyst^bg-J or Lyst^bg-2J females to (C3H-Lyst^bg-2J x B6-Lyst^bg-J)F2 males were cryopreserved in 1990.

Genetics

Lyst^bg-J

Allele Symbol: Lyst^bg-J

Allele Name	beige Jackson
Allele Type	Spontaneous
Allele Synonym(s)	bg^j
Gene Symbol and Name	Lyst, lysosomal trafficking regulator
Gene Synonym(s)
Strain of Origin	C57BL/6J
Chromosome	13
General Note	This remutation occurred spontaneously in the C57BL/6J strain at The Jackson Laboratory (J:5311). It has essentially identical effects to the original Lyst^bg mutation, and has been used extensively in defining locus effects.
Molecular Note	This allele is defined by a noncomplementation test with Lyst^bg. This mutation is the result of a 3 bp deletion in exon 54 causing an isoleucine deletion at codon 3741 near the carboxy terminus of the protein. This deletion affects the WD40 domain.
Mutations Made By	The Jackson Laboratory

Lyst^bg-2J

Allele Symbol: Lyst^bg-2J

Allele Name	beige 2 Jackson
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Lyst, lysosomal trafficking regulator
Gene Synonym(s)
Strain of Origin	Yellow stock
Chromosome	13
Molecular Note	This allele was identified by a noncomplementation test with Lyst^bg. Quantitative reverse transcription showed the molecular fault in the Lyst^bg-2J mutation may be an intronic retrotransposition event causing a five-fold decrease in transcription and mRNA instability.

a

Allele Symbol: a

Allele Name	nonagouti
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	old mutant of the mouse fancy
Chromosome	2
General Note	Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039
Molecular Note	Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for a^t-2Gso and A^w-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.

A

Allele Symbol: A

Allele Name	wild-type agouti
Allele Type	Spontaneous
Allele Synonym(s)	dark-bellied agouti
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	various
Chromosome	2
General Note	The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back.
Molecular Note	This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene.

T(10;13)199H

Marker Symbol: T(10;13)199H

Marker Synonym(s)
Chromosome(s)	13

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Lyst^bg-J related

Hematological Research
- Platelet Defects
  - platelet storage pool deficiency
- Immunological Defects
Internal/Organ Research
- Kidney Defects
  - lysosomal enzyme abnormalities
Mouse/Human Gene Homologs
- Chediak-Higashi syndrome
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - NK Cell Deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - NK Cell and Cd8 T Cell defects
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Immunodeficiency Associated with Other Defects
- Intracellular Signaling Molecules
Cardiovascular Research
- Atherosclerosis
Dermatology Research
- Color and White Spotting Defects
Metabolism Research

Genotype: Lyst^bg-2J related

Immunology, Inflammation and Autoimmunity Research
- Intracellular Signaling Molecules
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Immunodeficiency
Hematological Research
- Platelet Defects
  - platelet storage pool deficiency
- Immunological Defects
Internal/Organ Research
- Kidney Defects
  - lysosomal enzyme abnormalities
Mouse/Human Gene Homologs
- Chediak-Higashi syndrome
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - NK Cell Deficiency
Metabolism Research
Cardiovascular Research
- Atherosclerosis
Dermatology Research
- Color and White Spotting Defects

Mammalian Phenotype Terms by Genotype

References

Additional - a related

Additional - A related

Additional - Lyst^bg-2J related

Additional - Lyst^bg-J related

Additional - T(10;13)199H related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the B6C3 a/A-T(10;13)199H +/+ Lyst^bg-J/J or Lyst^bg-2J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000604 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

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Price

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Cryorecovery - Pricing

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Genetic overview

Marker(s)

Research Applications

Base Price

Detailed Description

Development

Lystbg-J

Allele Symbol: Lystbg-J

Lystbg-2J

Allele Symbol: Lystbg-2J

a

Allele Symbol: a

A

Allele Symbol: A

T(10;13)199H

Marker Symbol: T(10;13)199H

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Lystbg-J related

Genotype: Lystbg-2J related

Mammalian Phenotype Terms by Genotype

References

Additional - a related

Additional - A related

Additional - Lystbg-2J related

Additional - Lystbg-J related

Additional - T(10;13)199H related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Lyst^bg-J

Allele Symbol: Lyst^bg-J

Lyst^bg-2J

Allele Symbol: Lyst^bg-2J

Genotype: Lyst^bg-J related

Genotype: Lyst^bg-2J related

Additional - Lyst^bg-2J related

Additional - Lyst^bg-J related