Mice of this stock carry the T(10;13)199H reciprocal translocation and either Lystbg-J or Lystbg-2J.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Lyst | lysosomal trafficking regulator |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Lyst | lysosomal trafficking regulator |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | a | nonagouti |
Marker Symbol | Marker Name | |
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T(10;13)199H | reciprocal translocation, Chr 10 and 13, Harwell 199 |
Because males carrying the T(10;13)199H translocation are either sterile or die in utero, the translocation is propagated by mating females carrying the translocation to (C3H-Lystbg-2J x B6-Lystbg-J)F1 males. Agouti or non-agouti offspring will be carriers of the translocation and offspring having the beige coat color are non-carriers of the translocation.
The translocation was induced by X-irradiation of a (C3H x 101)F1 male, and imported from Harwell via Mary Lyon. Embryos produced by mating B6C3 a/A-T(10;13)199H +/+ -Lystbg-J or Lystbg-2J females to (C3H-Lystbg-2J x B6-Lystbg-J)F2 males were cryopreserved in 1990.
Allele Name | nonagouti |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 2 |
General Note | Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039 |
Molecular Note | Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type. |
Allele Name | beige Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | bgj |
Gene Symbol and Name | Lyst, lysosomal trafficking regulator |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 13 |
General Note | This remutation occurred spontaneously in the C57BL/6J strain at The Jackson Laboratory (J:5311). It has essentially identical effects to the original Lystbg mutation, and has been used extensively in defining locus effects. |
Molecular Note | This allele is defined by a noncomplementation test with Lystbg. This mutation is the result of a 3 bp deletion in exon 54 causing an isoleucine deletion at codon 3741 near the carboxy terminus of the protein. This deletion affects the WD40 domain. |
Mutations Made By | The Jackson Laboratory |
Allele Name | beige 2 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Lyst, lysosomal trafficking regulator |
Gene Synonym(s) | |
Strain of Origin | Yellow stock |
Chromosome | 13 |
Molecular Note | This allele was identified by a noncomplementation test with Lystbg. Quantitative reverse transcription showed the molecular fault in the Lystbg-2J mutation may be an intronic retrotransposition event causing a five-fold decrease in transcription and mRNA instability. |
Allele Name | wild-type agouti |
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Allele Type | Spontaneous |
Allele Synonym(s) | dark-bellied agouti |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | various |
Chromosome | 2 |
General Note | The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back. |
Molecular Note | This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene. |
Marker Synonym(s) | |
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Chromosome(s) | 13 |
When using the B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000604 in your Materials and Methods section.
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