Overview

These mice carry two linked, spontaneous mutations, waved 2 (Egfr^wa2) and vestigial tail (Wnt3a^vt); they display curly whiskers and short tails.

Genetic overview

Genetic Background	Generation

Egfr^wa2

Allele Type	Gene Symbol	Gene Name
Spontaneous	Egfr	epidermal growth factor receptor

A

Allele Type	Gene Symbol	Gene Name
Spontaneous	a	nonagouti

Allele Type	Gene Symbol	Gene Name
Spontaneous (Hypomorph)	Wnt3a	wingless-type MMTV integration site family, member 3A

Research Applications

Cancer Research
Dermatology Research
Developmental Biology Research
Endocrine Deficiency Research
Immunology, Inflammation and Autoimmunity Research
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for the spontaneous waved 2 mutation (Egfr^wa2) are recognizable at 2 to 3 days by curly whiskers. The first coat is waved but later coats are not; vibrissae usually remain curled and the guard hairs curved. Some homozygotes have eyelids open at birth. Fertile mutant females have impaired lactation. Mice homozygous for the vestigial tail spontaneous mutation (Wnt3a^vt) have very short tails, few presacral vertebrae, and abnormal formation of the lumbar vertebrae.

Development

The vt mutation in Wnt3a arose spontaneously in the C57BR background.

Control Suggestions

Untyped from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Egfr^wa2

Allele Symbol: Egfr^wa2

Allele Name	waved 2
Allele Type	Spontaneous
Allele Synonym(s)	wa2; wa-2; waved2
Gene Symbol and Name	Egfr, epidermal growth factor receptor
Gene Synonym(s)
Strain of Origin	STOCK ac
Chromosome	11
General Note	Found by Keeler in an ``abnormal corpus callosum'' stock at the Bussey Institution.
Molecular Note	A T-to-G transversion mutation in the sequences encoding the tyrosine kinase domain results in a substitution of a highly conserved valine with glycine at position 767 (P.V767G) in the third kinase subdomain.

A

Allele Symbol: A

Allele Name	wild-type agouti
Allele Type	Spontaneous
Allele Synonym(s)	dark-bellied agouti
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	various
Chromosome	2
General Note	The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back.
Molecular Note	This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene.

Wnt3a^vt

Allele Symbol: Wnt3a^vt

Allele Name	vestigial tail
Allele Type	Spontaneous (Hypomorph)
Allele Synonym(s)	vt
Gene Symbol and Name	Wnt3a, wingless-type MMTV integration site family, member 3A
Gene Synonym(s)
Strain of Origin	C57BR
Chromosome	11
Molecular Note	Examination of the Wnt3a gene by Southern blot, Northern blot on adult lung tissue and cDNA sequence analysis revealed no sign of an obvious mutation that could account for a mutant phenotype. However, this mutation and a targeted mutation, Wnt3a^tm1Amc, were demonstrated to be noncomplemeting alleles. Further evidence from whole mount in situ hybridization studies on embryos demonstrated that expression of the encoded protein is reduced in homozygous embryos, suggesting that this hypomorphic allele results from a mutation in a regulatory element.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Egfr^wa2 related

Cancer Research
- Growth Factors/Receptors/Cytokines
Dermatology Research
- Skin and Hair Texture Defects
Developmental Biology Research
- Neural Tube Defects
Endocrine Deficiency Research
- Skin Defects
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines
Neurobiology Research
- Neural Tube Defects

Genotype: Wnt3a^vt related

Developmental Biology Research
- Neural Tube Defects
Neurobiology Research
- Neural Tube Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Egfr^wa2/Egfr^wa2
B6EiC3Sn a/A-Egfr Wnt3a/J

cardiovascular system phenotype

enlarged aortic valve
- enlarged/thickened aortic valves

thick aortic valve

endocrine/exocrine gland phenotype

abnormal lactation
- reduction of milk within ducts of mammary glands and secretory vacuolation within lobules is less pronounced

abnormal mammary gland morphology
- small mammary glands and the ratio of gland to adipose tissue is reduced

integument phenotype

abnormal hair texture

abnormal lactation
- reduction of milk within ducts of mammary glands and secretory vacuolation within lobules is less pronounced

abnormal mammary gland morphology
- small mammary glands and the ratio of gland to adipose tissue is reduced

abnormal vibrissa morphology

Genotype: Wnt3a^vt/Wnt3a^vt
B6EiC3Sn a/A-Egfr Wnt3a/J

skeleton phenotype

cervical vertebral transformation
- homeotic transformation of cervical vertebra 2 (C2) to C1 is seen in some homzygotes

lumbar vertebral transformation
- transformation of L6 to sacral vertebra 1 (S1)

sacral vertebral transformation
- transformation of S4 to S3

thoracic vertebral transformation
- transformation of T13 to lumbar vertebra 1 (L1)
- transformation of thoracic vertebra 9 (T9) to T10

References

Additional References

Beland M; Pilon N; Houle M; Oh K; Sylvestre JR; Prinos P; Lohnes D. 2004. Cdx1 autoregulation is governed by a novel Cdx1-LEF1 transcription complex. Mol Cell Biol 24(11):5028-38PubMed: 15143193 MGI: J:91084
Fitch KR; McGowan KA; Van Raamsdonk CD; Fuchs H; Lee D; Puech A; Herault Y; Threadgill DW; De Angelis MH; Barsh GS. 2003. Genetics of dark skin in mice. Genes Dev 17(2):214-28PubMed: 12533510 MGI: J:81301
Greco TL; Takada S; Newhouse MM; McMahon JA; McMahon AP; Camper SA. 1996. Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development. Genes Dev 10(3):313-24PubMed: 8595882 MGI: J:31335
Luetteke NC; Phillips HK; Qiu TH; Copeland NG; Earp HS; Jenkins NA; Lee DC. 1994. The mouse waved-2 phenotype results from a point mutation in the EGF receptor tyrosine kinase. Genes Dev 8(4):399-413PubMed: 8125255 MGI: J:16986
Prinos P; Joseph S; Oh K; Meyer BI; Gruss P; Lohnes D. 2001. Multiple Pathways Governing Cdx1 Expression during Murine Development. Dev Biol 239(2):257-69PubMed: 11784033 MGI: J:72581
Sunnarborg SW; Hinkle CL; Stevenson M; Russell WE; Raska CS; Peschon JJ; Castner BJ; Gerhart MJ; Paxton RJ; Black RA; Lee DC. 2002. Tumor necrosis factor-alpha converting enzyme (TACE) regulates epidermal growth factor receptor ligand availability. J Biol Chem 277(15):12838-45PubMed: 11823465 MGI: J:75887
Threadgill DW; Dlugosz AA; Hansen LA; Tennenbaum T; Lichti U; Yee D; LaMantia C; Mourton T; Herrup K; Harris RC; Barnard JA; Yuspa SH; Coffey RJ; Magnuson T. 1995. Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype. Science 269(5221):230-4PubMed: 7618084 MGI: J:26833

Additional - A related

Additional - Egfr^wa2 related

Additional - Wnt3a^vt related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Sanger sequencing:Egfr-SEQ
- Genotyping resources and troubleshooting
Appearance
- black, wavy coat, tail defects
  Related Genotype: a/a Egfr^wa2 Wnt3a^vt/Egfr^wa2 Wnt3a^vt
  
  agouti, wavy coat, tail defects
  Related Genotype: A/? Egfr^wa2 Wnt3a^vt/Egfr^wa2 Wnt3a^vt
  
  black, normal coat and tail
  Related Genotype: a/ a Egfr^wa2 Wnt3a^vt/+ + or a/a ? ?/+ +
  
  agouti, normal coat and tail
  Related Genotype: A/? Egfr^wa2Wnt3a^vt/+ + or A/? ? ?/+ +
Citation
When using the B6EiC3Sn a/A-Egfr^wa2 Wnt3a^vt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000553 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for <wa2>, Heterozygous for Wnt3a<vt>

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Egfrwa2

Allele Symbol: Egfrwa2

A

Allele Symbol: A

Wnt3avt

Allele Symbol: Wnt3avt

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Egfrwa2 related

Genotype: Wnt3avt related

Mammalian Phenotype Terms by Genotype

Genotype: Egfrwa2/Egfrwa2B6EiC3Sn a/A-Egfr Wnt3a/J

cardiovascular system phenotype

endocrine/exocrine gland phenotype

integument phenotype

Genotype: Wnt3avt/Wnt3avtB6EiC3Sn a/A-Egfr Wnt3a/J

skeleton phenotype

References

Additional References

Additional - A related

Additional - Egfrwa2 related

Additional - Wnt3avt related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Egfr^wa2

Allele Symbol: Egfr^wa2

Wnt3a^vt

Allele Symbol: Wnt3a^vt

Genotype: Egfr^wa2 related

Genotype: Wnt3a^vt related

Genotype: Egfr^wa2/Egfr^wa2
B6EiC3Sn a/A-Egfr Wnt3a/J

Genotype: Wnt3a^vt/Wnt3a^vt
B6EiC3Sn a/A-Egfr Wnt3a/J

Additional - Egfr^wa2 related

Additional - Wnt3a^vt related