These mice carry a spontaneous mutation at the Hps5 locus characterized by unpigmented eyes at birth that later develop into a dark, ruby color. Black and yellow pigments of the coat are diluted. The mice show high concentrations of kidney lysosomal enzymes.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Hps5 | HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 |
Ruby-eye-2-J mice have abnormally high concentrations of kidney lysosomal enzymes. Concentrations of kidney non-lysosomal enzymes and liver and serum lysosomal enzymes are normal. There is a decreased rate of secretion of lysosomal enzymes from kidney into urine. These mice should serve as useful models for the study of the biogenesis, structure, and processing of these and other subcellular organelles.
The ruby-eye 2 Jackson mutation arose spontaneously in C57BL/6J when that strain was at generation F98 and was maintained by sibling mating. In 1981 homozygous males at F34 were bred with C57BL/6J females to generate embryos for cryopreservation.
Allele Name | ruby-eye 2 Jackson |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | ru2J; ru-2J; ru-2J; ruby-type |
Gene Symbol and Name | Hps5, HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 7 |
Molecular Note | This spontaneous mutation was shown to be an allele of Hpsru-2 by complementation testing, and thus an allele of Hps5ru2. Subsequent sequence analysis identified the replacement of G with TT in codon 757, putatively resulting in a frameshift mutation that precludes the translation of 311 carboxy terminal amino acids. Northern blot analysis indicated that the mutation did not affect the expression pattern of the gene. |
When using the ruby-eye 2 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000542 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for ru2<J> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.