Overview

The CXB set of RI strains is used in the genetic analysis of numerous complex or potentially complex physiologic phenotypes including differences in thyroid function (Graves' disease) and pulmonary inflammation as well as behavioral phenotypes including avoidance, exploration and locomotor activity. The CXB set is derived from the BALBc/ByJ (Stock No. 001026) and C57BL/6ByJ (Stock No. 001139) progenitor strains.

Genetic overview

Genetic Background	Generation

Fgfr2^svs

Allele Type	Gene Symbol	Gene Name
Spontaneous	Fgfr2	fibroblast growth factor receptor 2

Hld

Allele Type	Gene Symbol	Gene Name
Spontaneous	Hld	hippocampal lamination defect

Ahr^b-2

Allele Type	Gene Symbol	Gene Name
Not Applicable	Ahr	aryl-hydrocarbon receptor

Crb1^rd8

Allele Type	Gene Symbol	Gene Name
Spontaneous	Crb1	crumbs family member 1, photoreceptor morphogenesis associated

View Genetics

Research Applications

Research Tools
Neurobiology Research
Reproductive Biology Research
Developmental Biology Research
Sensorineural Research
Cell Biology Research
Mouse/Human Gene Homologs

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

This strain is homozygous for a naturally occurring insertion in Fgfr2 resulting in aberrant splicing, however, our sequencing data indicates that the insert orientation is opposite to the published information (Kuslak 2007). The mutation, seminal vesicle shape (svs), is associated with abnormal morphology of the prostate and seminal vesicles.

Detailed Description

The CXB set of RI strains is used in the genetic analysis of numerous complex or potentially complex physiologic phenotypes including differences in thyroid function (Graves' disease) and pulmonary inflammation as well as behavioral phenotypes including avoidance, exploration and locomotor activity.

The CXB set is so small that markers on different chromosomes occasionally have almost precisely the same SDP. This produces high non-syntenic association and false linkage between variance in phenotypes and genotypes. Please examine the correlation coefficients of markers close to interest loci with ALL other markers to evaluate the risk of non-syntenic association.

The strain distribution pattern (SDP) for the CXB RI strains is available through the Mouse Genome Informatics

Contributed Data Sets and Gene Network.

Additional tools and information are presented through the Mouse Phenome Database

Specialized Strain Panel Query Form, and

Gene Network.

Like BALB/cByJ, this recombinant inbred carries the mutation hippocampal lamination defect or Hld, an allele responsible for abnormal neuronal migration to the pyramidal cell layer (Nowakowski RS, et al, Jnl Neurogen, 1984).

Development

The original 11 CXB recombinant inbred (RI) lines were generated at the National Institutes of Health by Dr. Donald Bailey (labcode By) starting in 1959. After moving to The Jackson Laboratory in 1967, an additional set of 6 strains was created with the help of Jo Hilgers (Labcode Hi). The CXB set is derived from the BALBc/ByJ (Stock No. 001026) and C57BL/6ByJ (Stock No. 001139) progenitor strains. CXB1 through CXB7 originally were designated using letters. Several of the original strains are extinct. The Jackson Laboratory currently distributes 7 of the original By strains and 6 of the Hi strains.

Selected References

Bailey DW. 1971. Recombinant-inbred strains. An aid to finding identity, linkage, and function of histocompatibility and other genes. Transplantation 11(3):325-7PubMed: 5558564 MGI: J:17649
Williams RW; Gu J; Qi S; Lu L. 2001. The genetic structure of recombinant inbred mice: high-resolution consensus maps for complex trait analysis. Genome Biol 2(11):1-18PubMed: 11737945 MGI: J:73062

View All References

Genetics

Fgfr2^svs

Allele Symbol: Fgfr2^svs

Allele Name	seminal vesicle shape
Allele Type	Spontaneous
Allele Synonym(s)	svs
Gene Symbol and Name	Fgfr2, fibroblast growth factor receptor 2
Gene Synonym(s)
Strain of Origin	CXB5/By
Chromosome	7
General Note	This recessive autosomal mutation apparently occurred early in the inbreeding of the Essex CXB recombinant inbred lines, as the abnormality was not present in either of the parental strains, C57BL/6ByEss or BALB/cByEss, but is present in both CXBI/ByJax and CXBI/ByLac J:9441.
Molecular Note	An insertion of a 491-bp mouse leukemia virus long terminal repeat (MLV-LTR) sequence into intron 10 results in aberrant alternative splicing of the transcript with exclusion from most mature mutant mRNA species of exon 8IIIb, which encodes the domain required for specific binding to FGF ligands in developing prostate and seminal vesicle mesenchyme.

Hld

Allele Symbol: Hld

Allele Name	hippocampal lamination defect
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Hld, hippocampal lamination defect
Gene Synonym(s)
Strain of Origin	BALB/cJ
Chromosome	UN

Ahr^b-2

Allele Symbol: Ahr^b-2

Allele Name	b-2 variant
Allele Type	Not Applicable
Allele Synonym(s)	Ah^b-2; Ah^h
Gene Symbol and Name	Ahr, aryl-hydrocarbon receptor
Gene Synonym(s)
Strain of Origin	BALB/cBy
Chromosome	12
General Note	C57BL/6 carries the responsive Ahr^b allele; DBA/2 carries nonresponsive Ahr^d. Heterozygotes (Ahr^b/Ahr^d) are responsive (J:5282). Later work identified a second (J:8895) and later a third (J:22144) allele conferring response. Thus the allele in C57, C58, and MA/My strains is now Ahr^b-1; Ahr^b-2 is carried by BALB/cBy, A, and C3H; and Ahr^b-3 by Mus spretus, M. caroli, and MOLF/Ei. The nonresponsive strains AKR, DBA/2, and 129 carry Ahrd (J:22144). Nucleotide and amino acid sequence differences between Ahr^b-1 and Ahr^d have been determined (J:17460). Strain of origin - this allele was found in BALB/cByJ, A/J, C3H/HeJ, CBA strains
Molecular Note	This allele encodes a high affinity, heat labile, 104 kDa receptor containing 848 amino acids. Sequencing studies of cDNA from C57BL/6J congenic mice homozygous for this allele identified nucleotide substitutions in the ORF that would cause 5 amino acid differences between the C57BL/6J and BALB/cBy peptides, and 2 amino acid differences between the BALB/cBy and DBA/2J peptides. A T to C transition in exon 11 replaces the opal termination codon in the C57BL/6J allele with an arginine codon in the BALB/cBy allele. This change would extend translation of the BALB/cBy mRNA by 43 amino acids, accounting for the larger size of the peptide produced by this allele (104 kDa, vs 95 kDa for the C57BL/6J allele).

Crb1^rd8

Allele Symbol: Crb1^rd8

Allele Name	retinal degeneration 8
Allele Type	Spontaneous
Allele Synonym(s)	Crb1^6N; nmf144; Rd8^-
Gene Symbol and Name	Crb1, crumbs family member 1, photoreceptor morphogenesis associated
Gene Synonym(s)
Strain of Origin	C57BL/6By or C57BL/6N
Chromosome	1
Molecular Note	The mutation in the rd8 mouse has been identified as a single base deletion of a C (G on forward strand) at coding nucleotide 3481 in the gene. This deletion causes a frame shift and a premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein after amino acid 1207. This mutation has been found to be present in all sublines of C57BL/6N and in C57BL/6ByJ, but not in any C57BL/6J subline. It occurred sometime between transfer of mice from JAX to NIH, in 1951, and from NIH to Donald Bailey, in 1961.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis
  - Gene Mapping
Neurobiology Research
- Behavioral and Learning Defects
Reproductive Biology Research
- Endocrine Deficiencies Affecting Gonads

Genotype: Crb1^rd8 related

Developmental Biology Research
- Eye Defects
Sensorineural Research
- Retinal Degeneration
Cell Biology Research
- Defects in Cell Adhesion Molecules
Mouse/Human Gene Homologs
- Retinitis pigmentosa 12; Leber congenital amaurosis (CLA)

Mammalian Phenotype Terms by Genotype

Phenotype Information

Wellcome Trust Centre for Human Genetics: Mouse Recombinant Inbred Line (RIL) Genotype Data for CXB RI Line

References

Bailey DW. 1971. Recombinant-inbred strains. An aid to finding identity, linkage, and function of histocompatibility and other genes. Transplantation 11(3):325-7PubMed: 5558564 MGI: J:17649
Williams RW; Gu J; Qi S; Lu L. 2001. The genetic structure of recombinant inbred mice: high-resolution consensus maps for complex trait analysis. Genome Biol 2(11):1-18PubMed: 11737945 MGI: J:73062

Additional References

Aliesky HA; Pichurin PN; Chen CR; Williams RW; Rapoport B; McLachlan SM. 2006. Probing the genetic basis for thyrotropin receptor antibodies and hyperthyroidism in immunized CXB recombinant inbred mice. Endocrinology 147(6):2789-800PubMed: 16543368 MGI: J:109467
De Maeyer E; De Maeyer-Guignard J; Bailey DW. 1975. Effect of mouse genotype on interferon production. I. Lines congenic at the If-1 locus. Immunogenetics 1:438-443MGI: J:4430
Hamidi S; Aliesky HA; Williams RW; Rapoport B; McLachlan SM. 2013. Genetic linkages for thyroxine released in response to thyrotropin stimulation in three sets of recombinant inbred mice provide evidence for shared and novel genes controlling thyroid function. Thyroid 23(3):360-70PubMed: 22988948 MGI: J:204730
McLachlan SM; Lu L; Aliesky HA; Williams RW; Rapoport B. 2011. Distinct genetic signatures for variability in total and free serum thyroxine levels in four sets of recombinant inbred mice. Endocrinology 152(3):1172-9PubMed: 21209025 MGI: J:173883
Nowakowski RS. 1984. The mode of inheritance of a defect in lamination in the hippocampus of BALB/c mice. J Neurogenet 1(3):249-58PubMed: 6536729 MGI: J:7947
Poland A; Glover E; Taylor BA. 1987. The murine Ah locus: a new allele and mapping to chromosome 12. Mol Pharmacol 32(4):471-8PubMed: 2823093 MGI: J:8895

Additional - Ahr^b-2 related

Additional - Crb1^rd8 related

Additional - Fgfr2^svs related

Additional - Hld related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- albino
  Related Genotype: a/a Tyrp1^b/Tyrp1^b Tyr^c/Tyr^c
Citation
When using the CXB5/ByJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #000355 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

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Price

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> >	Inbred, 1 pair minimum will be supplied

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Frozen Mouse Embryo	CXB5/ByJ Frozen Embryos	$2595.00
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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Selected References

Fgfr2svs

Allele Symbol: Fgfr2svs

Hld

Allele Symbol: Hld

Ahrb-2

Allele Symbol: Ahrb-2

Crb1rd8

Allele Symbol: Crb1rd8

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Crb1rd8 related

Mammalian Phenotype Terms by Genotype

Phenotype Information

References

Additional References

Additional - Ahrb-2 related

Additional - Crb1rd8 related

Additional - Fgfr2svs related

Additional - Hld related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Fgfr2^svs

Allele Symbol: Fgfr2^svs

Ahr^b-2

Allele Symbol: Ahr^b-2

Crb1^rd8

Allele Symbol: Crb1^rd8

Genotype: Crb1^rd8 related

Additional - Ahr^b-2 related

Additional - Crb1^rd8 related

Additional - Fgfr2^svs related