In May 2021, Stock No. 000330 was discontinued due to very poor breeding and difficulty with cryopreservation. Please search the JAX® Mice database for other strains which may be suitable for your studies, or contact Technical Support for assistance.
The BXD set of RI strains is used in the genetic analysis of numerous complex or potentially complex physiologic phenotypes. The BXD strains are derived from the C57BL/6J (Stock No. 000664) and DBA/2J (Stock No. 000671) progenitor strains.
The BXD RI strains are used to study the genetics of behavioral phenotypes including alcohol and drug addiction, stress, and locomotor activity.The BXD set of RI strains also are used in the genetic analysis of numerous complex or potentially complex physiologic phenotypes including differences in organ weight and bone mineral density.
The strain distribution pattern (SDP) for the BXD RI strains is available through the Mouse Genome Informatics Contributed Data Sets and Gene Network. Additional tools and information are presented through the Mouse Phenome Database Specialized Strain Panel Query Form, and Gene Network.
The Jackson Laboratory live colony was a challenging breeder / very poor breeder. It exhibited a high incidence of non-productive matings and when productive, the litters were small [May 2021]. See our General Husbandry Tips.
The BXD set of RI strains was developed in the laboratory of Benjamin Taylor and was transferred to The Jackson Laboratory from Dr. Taylor's research colony upon his retirement. Of 42 BXD RI strains originally derived from the C57BL/6J (Stock No. 000664) and DBA/2J (Stock No. 000671), a number have become extinct over the years. The other BXD RI strains now being distributed are listed under "Related Recombinant Inbred Strains."
In 1982/83 after BXD-20/Ty had been intercrossed approximately 48 times (uncertain of exact date and filial generation number) one F48 BXD20/Ty male was outcrossed once to a single C57BL/6J female. The N1 offspring from this cross were then backcrossed to their sire, and this process was repeated 4 times. In total the outcross to C57BL/6J was followed by 4 consecutive backcrosses to the same F48 BXD-20/Ty male. The line was then intercrossed and after 20 additional generations of inbreeding efforts began to cryopreserve the strain. The generation number of the cryopreserved mice F48N5F75+10p.
|Allele Name||a variant|
|Allele Type||Spontaneous (Not Applicable)|
|Gene Symbol and Name||B2m, beta-2 microglobulin|
|Strain of Origin||multiple strains|
|Molecular Note||The a variant is slightly slower running on SDS PAGE and was found to have an aspartic acid at amino acid position 85. This allele is found in A, BALB/c, LP and most other strains.|