In 1933, or shortly thereafter, the waved 2 mutation arose spontaneously in the "abnormal Corpus callosum stock" held by Dr. Clyde Keeler at The Bussey Institute of Harvard Medical School. In 1935 waved 2 (Egfrwa2) was received from Dr. Reed by Dr. G.D. Snell at the Jackson Laboratory. It was crossed to shaker 2 (Myo15sh2) mice of Dr. C.C. Little at The Jackson Laboratory and waved 2 shaker 2 (Egfrwa2 Myo15sh2) mice were crossed to flexed-tail (f) mice received from the Bussey Institute in 1936. All three mutations were maintained in this stock. In 1947 this stock was returned after the fire to Dr. Snell from Dr. Walter Heston. The stock was maintained by non-sibling within-stock matings until 1960 when one outcross was made to C3H/HeJ. The stock was then sibling mated for 13 generations before 3 crosses were made to C57BL/6J, via backcross-intercross, and the stock was then non-sibling within-stock mated and in 1969 two lines were selected, Myo15sh2 and Egfrwa2 and flexed was dropped from these lines. In the Egfrwa2 line a cross was made to B6.Cg-bc, a congenic strain then at N5, and the resulting stock, carrying a and Egfrwa2 but not bc, was sibling mated to maintain Egfrwa2 by forced heterozygosis. It was cryopreserved in 1986 with the common parent at F53.
