Overview

Also Known As:Dancer

This inbred mutant strain carries the Dancer mutation (Tbx10^Dc).

Genetic overview

Genetic Background	Generation

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Research Applications

Dermatology Research
Developmental Biology Research
Neurobiology Research
Sensorineural Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

This strain is segregating for Tbx10^Dc.

Detailed Description

Mice heterozygous for the semidominant dancer mutation exhibit head tossing and circling behavior. Heterozygotes are not deaf, but have defects in the vestibular region (Wenngren et al., 1989). Homozygotes die at birth with cleft lip and cleft palate (Deol et al., 1966). Strain background influences cleft lip/palate expression in heterozygotes (Trasler et al., 1982). Positional cloning of Dc revealed a 3.3 kb insertion of the Tebp gene into the first intron of Tbx10. The insertion causes ectopic expression of a Tebp-Tbx10 chimeric transcript (Bush et al., 2004).

Development

Dancer (Dc) arose as a spontaneous mutation in the C3H/He obese strain (C3H/He-Lep^ob) at N4 at The Jackson Laboratory in 1956. A dancer and normal sibling of the first appearing dancer mouse were mated and their dancer off spring (Dc/+) was crossed once to C3H/HeJ . The F1's of this cross were sibling mated with an unaffected (+/+) male that came from from the intercross of a dancer (Dc/+) male and an unaffected (+/+) female. The strain was then maintained in this manner and was cryopreserved by mating heterozygous males to +/+ females or vice versa at generation F124 in 1990, and later freezes in 2001 and 2004 were recovered from the original freeze.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Tbx10^Dc

Allele Symbol: Tbx10^Dc

Allele Name	dancer
Allele Type	Spontaneous
Allele Synonym(s)	Dc
Gene Symbol and Name	Tbx10, T-box 10
Gene Synonym(s)
Strain of Origin	C3.Cg-Lep^ob
Chromosome	19
Molecular Note	The mutation in this allele comprises a small translocation insertion that placed a 3.3 kb fragment of Chr 10 into intron 1 of the Tbx10 gene. This inserted sequence includes exon 1 of the Tebp gene. RT-PCR analysis of Tbx10 expression revealed that the full-length trancript is expressed normally in mutant mice, and that a chimeric transcript consisting of the Tebp exon 1 of the inserted sequence fused in-frame to exon 2 of the Tbx10 gene is expressed ectopically. This chimeric transcript is predicted to encode a trucated, but functional protein that is similar to a normal variant.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Tbx10^Dc related

Dermatology Research
- Color and White Spotting Defects
Developmental Biology Research
- Neurodevelopmental Defects
- Craniofacial and Palate Defects
  - cleft palate and cleft lip
Neurobiology Research
- Neurodevelopmental Defects
- Hearing Defects
Sensorineural Research
- Hearing Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tbx10^Dc/Tbx10^Dc
involves: C3H/HeJ

craniofacial phenotype

cleft upper lip
- unilateral or bilateral cleft lip

cleft palate

digestive/alimentary phenotype

cleft palate

growth/size/body region phenotype

cleft palate

cleft upper lip
- unilateral or bilateral cleft lip

mortality/aging

neonatal lethality, complete penetrance
- homozyogtes die within a few hours of birth and 9 of 40 newborns from heterozygous intercrosses have this inviable phenotype

Genotype: Tbx10^Dc/Tbx10⁺
involves: C3H/HeJ

craniofacial phenotype

abnormal bony labyrinth

cleft palate
- Background Sensitivity - does not occur spontaneously but can be induced on this background by treatment of pregnant mothers with 6-aminonicotinamide

digestive/alimentary phenotype

cleft palate
- Background Sensitivity - does not occur spontaneously but can be induced on this background by treatment of pregnant mothers with 6-aminonicotinamide

growth/size/body region phenotype

cleft palate
- Background Sensitivity - does not occur spontaneously but can be induced on this background by treatment of pregnant mothers with 6-aminonicotinamide

decreased body size
- smaller than wild-type littermates by 1 week of age

hearing/vestibular/ear phenotype

abnormal crista ampullaris morphology
- when narrowing is found in the superior semicircular duct, the crista is either missing or poorly formed, and even when normal in appearance the crista is often shifted in its location

abnormal ear development
- al, slants in the medial direction, and is foreshortened posteriorly
- by embryonic day 12 the utriculo-saccular folds are abnormal in appearance, the utricular nerve is always missing, the nerves of the superior and lateral cristae are sometimes missing, and the accessory nerve from the superior vestibular ganglion sometimes branches to one of the critae and is always thinner than normal
- by embryonic day 16 the absence of the utricular valve and saccular duct is evident
- by embryonic day 10 the acoustic ganglion is smaller and does not curve around to reach the anterior aspect of the vesicle, and the epithelium on the antero-lateral aspect is not thickened as in normal embryos
- by embryonic day 11 the nerve that would normally innervate the epithelium destined to become the utricular macula and superior lateral cristae is deficient and aberrent or entirely lacking
- s branches to one of the critae and is always thinner than normal
- by embryonic day 13 the shape and size of the utricle and saccule are abnormal, the utricle has no nerve supply and no macula, the nerves of the superior and lateral semicircular ducts are often missing, and the utriculo-saccular fold is thicker than normal, slants in the medial direction, and is foreshortened posteriorly

abnormal semicircular canal ampulla morphology

abnormal superior semicircular canal morphology
- the majority of heterozygotes have a unilateral or bilateral severe narrowing or complete obliteration of the lumen of the ampullar end of the superior semicircular duct and constriction of the bony canal in this region

abnormal utricular macula morphology
- in all dancer mice the utricular macula and utricular branch of the superior vestibular nerve are entirely missing

small utricle
- the utricle is smaller than normal, particularly dorso-ventrally

abnormal lateral semicircular canal morphology
- this abnormality is more severe in the lateral than the superior semicircular canal
- the majority of heterozygotes have a unilateral or bilateral severe narrowing, complete obliteration of the lumen, or absence of the ampullar end of the lateral semicircular duct and constriction or absence of the bony canal in this region

abnormal semicircular canal morphology

abnormal vestibular saccule morphology
- the free wall of the saccule is sometimes highly convoluted
- the saccule, affected in all heterozygotes, does not extent dorsally as far as the utricle and is attached to the utricle by a thick strand of cells without any lumen

abnormal vestibular labyrinth morphology

abnormal bony labyrinth

abnormal inner ear vestibule morphology

small vestibular saccule
- the saccule is smaller and narrower than normal

abnormal utricle morphology

integument phenotype

head spot
- most dancer mutants have a white spot, varying in size, in the middle of the head

mammalian phenotype

hearing/vestibular/ear phenotype
- Normal - heterozygotes are not deaf, the cochlea appears normal

nervous system phenotype

abnormal vestibular nerve morphology
- along with the utricular macula, the utricular branch of the superior vestibular nerve is entirely missing
- the lateral vestibular nerve rarely follows a normal course, and the nerve is very thin in instances when the crista is small
- the pathway of the nerve from the superior vestibular ganglion to the crista rarely takes a normal course along the base of the utricle, but often passes through the otic capsule and tympanic cavity, and is often branched
- the superior vestibular nerve enters the saccular macula near the center of the macula, rather than the dorsal border

small vestibular ganglion
- the vestibular ganglion does not extend much beyond the superior border of the saccular macula and has fewer, less densely packed cells

vestibular ganglion hypoplasia

pigmentation phenotype

head spot
- most dancer mutants have a white spot, varying in size, in the middle of the head

skeleton phenotype

abnormal bony labyrinth

behavior/neurological phenotype

head tossing
- heterozygotes make jerking movements of the head in both horizontal and vertical planes

impaired swimming

head tilt
- some, but not all, may hold their heads tilted to the side

hyperactivity
- evident by 1 week of age and increasing by 2 weeks of age

circling
- similar circling behavior as that of waltzer mutants but with less head movement

abnormal gait
- by 2 weeks of age heterozygotes decelop a weaving gait with bi-directional circling

abnormal postural reflex
- when lifted by the tail at approximately 3 days of age a heterozygote will hump its back instead of arching it, retract the front legs and bend the head toward the belly
- when lifted by the tail as an adult, a heterozygote will twist and turn and throw its body about

bidirectional circling

The following phenotype relates to a compound genotype created using this strain

Genotype: Tbx10^Dc/Tbx10^Dc
DC/LeJ

craniofacial phenotype

failure of palatal shelf elevation
- at E16.5, palatal shelves fail to elevate

cleft secondary palate
- accompanies cleft lip and evident at embryonic day 16.5

cleft upper lip
- bilateral cleft lip evident at embryonic day 16.5

digestive/alimentary phenotype

failure of palatal shelf elevation
- at E16.5, palatal shelves fail to elevate

cleft secondary palate
- accompanies cleft lip and evident at embryonic day 16.5

growth/size/body region phenotype

cleft secondary palate
- accompanies cleft lip and evident at embryonic day 16.5

failure of palatal shelf elevation
- at E16.5, palatal shelves fail to elevate

cleft upper lip
- bilateral cleft lip evident at embryonic day 16.5

vision/eye phenotype

delayed eyelid fusion

eyelids open at birth
- in some homozygotes

References

Additional References

Deol MS; Lane PW. 1966. A new gene affecting the morphogenesis of the vestibular part of the inner ear in the mouse. J Embryol Exp Morphol 16(3):543-58PubMed: 5297683 MGI: J:5022
Saridaki A; Ferraz C; Demaille J; Scherer G; Roux AF. 2000. Genomic sequencing reveals the structure of the Kcnk6 and map3k11 genes and their close vicinity to the sipa1 gene on mouse chromosome 19. Cytogenet Cell Genet 89(1-2):85-8PubMed: 10894943 MGI: J:63233
Trasler DG; Kemp D; Trasler TA. 1984. Increased susceptibility to 6-aminonicotinamide-induced cleft lip of heterozygote Dancer mice. Teratology 29(1):101-4PubMed: 6230750 MGI: J:7364
Trasler DG; Leong S. 1982. Mitotic index in mouse embryos with 6-aminonicotinamide-induced and inherited cleft lip. Teratology 25(2):259-65PubMed: 7101202 MGI: J:6819

Additional - Tbx10^Dc related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous males may be bred to wildtype mice from the colony or to C3H/HeJ inbred mice (Stock No. 000659). Heterozygous Dc/+ females make poor breeders. Homozygotes die at birth with cleft lip and cleft palate.
- Additional Breeding and Husbandry Support
Citation
When using the DC/LeJ mouse strain in a publication, please include JAX stock #000252 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous or Wild-type for Dc

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Dancer

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Control Suggestions

Additional Information

Tbx10Dc

Allele Symbol: Tbx10Dc

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Tbx10Dc related

Mammalian Phenotype Terms by Genotype

Genotype: Tbx10Dc/Tbx10Dcinvolves: C3H/HeJ

craniofacial phenotype

digestive/alimentary phenotype

growth/size/body region phenotype

mortality/aging

Genotype: Tbx10Dc/Tbx10+involves: C3H/HeJ

craniofacial phenotype

digestive/alimentary phenotype

growth/size/body region phenotype

hearing/vestibular/ear phenotype

integument phenotype

mammalian phenotype

nervous system phenotype

pigmentation phenotype

skeleton phenotype

behavior/neurological phenotype

Genotype: Tbx10Dc/Tbx10DcDC/LeJ

craniofacial phenotype

digestive/alimentary phenotype

growth/size/body region phenotype

vision/eye phenotype

References

Additional References

Additional - Tbx10Dc related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Tbx10^Dc

Allele Symbol: Tbx10^Dc

Genotype: Tbx10^Dc related

Genotype: Tbx10^Dc/Tbx10^Dc
involves: C3H/HeJ

Genotype: Tbx10^Dc/Tbx10⁺
involves: C3H/HeJ

Genotype: Tbx10^Dc/Tbx10^Dc
DC/LeJ

Additional - Tbx10^Dc related