The ae mutation was discovered by W.F. Hollander and J.W. Gowen among descendants of an irradiated male albino mouse of the S strain then maintained at Iowa State College and a female of the multiple-recessive P testing stock. A single black, typical non-agouti male produced by these matings was bred to three AY/A+ females (of unidentified background), yielding five male offspring that survived to maturity, three of which proved fertile. Each of these was bred to a female homozygous for the black-and-tan (a t) allele, which is recessive to both AY and A+. Black-and-tan offspring of this cross were bred among themselves, as were progeny of the latter crosses. In one of the resultant lines, the black mice had an extreme non-agouti phenotype, having no yellow hairs. This line contributed to the background of the inbred strain AEJ, which carries ae. Gdf5bp-H occurred spontaneously in the PCT multiple recessive stock at Harwell, U.K. It was backcrossed onto AEJ/GnLe-ae for 5 generations, and the stock has since been maintained by brother-sister inbreeding.
|Allele Name||extreme nonagouti|
|Allele Type||Radiation induced|
|Gene Symbol and Name||a, nonagouti|
|Strain of Origin||(P x S)F1|
|General Note||The ae mutation was found among descendants of an irradiated mouse. ae is recessive to all other alleles except al.|
|Allele Name||brachypodism Harwell|
|Gene Symbol and Name||Gdf5, growth differentiation factor 5|
|Strain of Origin||PCT|
|General Note||This remutation arose spontaneously at Harwell in the PCT multiple recessive stock (J:13391).|
|Molecular Note||This allele was identified by a noncomplementation test with Gdf5bp.|
When using the AEJ.Cg-ae +/a Gdf5bp-H/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000251 in your Materials and Methods section.