AEJ.Cg-a^e +/a Gdf5^bp-H/J

Stock number: 000251
Research areas: Cell Biology Research, Dermatology Research, Developmental Biology Research, Mouse/Human Gene Homologs

Development

The a^e mutation was discovered by W.F. Hollander and J.W. Gowen among descendants of an irradiated male albino mouse of the S strain then maintained at Iowa State College and a female of the multiple-recessive P testing stock. A single black, typical non-agouti male produced by these matings was bred to three A^Y/A⁺ females (of unidentified background), yielding five male offspring that survived to maturity, three of which proved fertile. Each of these was bred to a female homozygous for the black-and-tan (a ^t) allele, which is recessive to both A^Y and A⁺. Black-and-tan offspring of this cross were bred among themselves, as were progeny of the latter crosses. In one of the resultant lines, the black mice had an extreme non-agouti phenotype, having no yellow hairs. This line contributed to the background of the inbred strain AEJ, which carries a^e. Gdf5^bp-H occurred spontaneously in the PCT multiple recessive stock at Harwell, U.K. It was backcrossed onto AEJ/GnLe-a^e for 5 generations, and the stock has since been maintained by brother-sister inbreeding.

Allele

Symbol: a^e
Name: extreme nonagouti
MGI accession ID: MGI:1855939
Generation method: Radiation induced
Marker symbol: a
Marker name: nonagouti
Marker synonyms: agouti; agouti signal protein; AGSW; AGTI; AGTIL; As; ASP; SHEP9
Chromosome: 2
Strain of origin: (P x S)F1
General note: The a^e mutation was found among descendants of an irradiated mouse. a^e is recessive to all other alleles except a^l.

Allele

Symbol: Gdf5^bp-H
Name: brachypodism Harwell
MGI accession ID: MGI:1855973
Generation method: Spontaneous
Synonyms: ^bp-H
Marker symbol: Gdf5
Marker name: growth differentiation factor 5
Marker synonyms: BDA1C; BMP14; BMP-14; bp; brp; cartilage-derived morphogenetic protein-1; Cdmp1; CDMP-1; DUPANS; LAP4; LAP-4; OS5; SYM1B; SYNS2
Chromosome: 2
Strain of origin: PCT
Molecular note: This allele was identified by a noncomplementation test with Gdf5^bp.
General note: This remutation arose spontaneously at Harwell in the PCT multiple recessive stock (J:13391).