Overview

Mice homozygous for the swaying spontaneous mutation (Wnt1^sw) sway to one side or the other when attempting to move and may then pivot clockwise or counterclockwise around their rear legs. Homozygous mutant mice display marked ataxia and hypertonia that is probably attributable to malformations of the anterior vermis of the cerebellum and of the colliculi. The cerebellum is divided on the midline by a deep dorsal sagittal fissure extending from the leptomeninges down to the level of the fourth ventricle. The probable cause is failure of the midline fusion of the cerebellum.

Genetic overview

Genetic Background	Generation

a

Allele Type	Gene Symbol	Gene Name
Spontaneous	a	nonagouti

Wnt1^sw

Allele Type	Gene Symbol	Gene Name
Spontaneous	Wnt1	wingless-type MMTV integration site family, member 1

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Research Applications

Developmental Biology Research
Neurobiology Research

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Base Price

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$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for the swaying spontaneous mutation (Wnt1^sw) sway to one side or the other when attempting to move and may then pivot clockwise or counterclockwise around their rear legs. Homozygous mutant mice display marked ataxia and hypertonia that is probably attributable to malformations of the anterior vermis of the cerebellum and of the colliculi. The cerebellum is divided on the midline by a deep dorsal sagittal fissure extending from the leptomeninges down to the level of the fourth ventricle. The probable cause is failure of the midline fusion of the cerebellum.

Development

The mutation swaying (Wnt1^sw) arose spontaneously in a cross between mahoganoid (Mgrn1^md), which arose in C3H/HeJ, and downless Jackson (Edar^dl-J), which arose in a cross of two silver stocks at the Jackson Laboratory in 1966. It was sibling mated for 2 generations then a homozygous swaying female was crossed to a C57BL/6J male. The swaying mutation was backcrossed to C57BL/6J for 5 more generations either by mating a progeny tested heterozygous (sw) male or female to a C57BL/6J or by ovary transplant. In 1971 a C57BL/6J velvet coat (Ve) male at N13 was crossed to a host carrying a C57BL/6J sw/sw ovary. The pairs, Ve +/+ sw, were mated at N7 and the stock was maintained in this manner to N7F10. Because of poor breeding a cross to C3HeB/FeJ-a/a was made with a sw/sw ovary transplant. The stock was then maintained by the cross-intercross method using a homozyogus ovary transplant host crossed with a B6C3Fe-a/a F1 male. The stock was frozen in 1982 by mating heterozygous males to heterozygous females at N13. It was thawed and re-frozen in 1990 by intercrossing heterozygotes and again in 2003 by IVF using homozygous sperm and +/? females at [F3NE16p]+F2.

Genetics

a

Allele Symbol: a

Allele Name	nonagouti
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	old mutant of the mouse fancy
Chromosome	2
General Note	Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039
Molecular Note	Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for a^t-2Gso and A^w-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.

Wnt1^sw

Allele Symbol: Wnt1^sw

Allele Name	swaying
Allele Type	Spontaneous
Allele Synonym(s)	sw
Gene Symbol and Name	Wnt1, wingless-type MMTV integration site family, member 1
Gene Synonym(s)
Strain of Origin	STOCK Atrn^mg Edar^dl-J
Chromosome	15
Molecular Note	The mutation is a deletion of 1 guanosine residue from a run of 4 consecutive guanosines beginning at genomic nucleotide 1888. This mutation is predicted to cause a frameshift mutation resulting in a stop codon 10 codons downstream from the deletion. The predicted protein would lack critical amino acids for biological activity.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Wnt1^sw related

Developmental Biology Research
- Neural Tube Defects
Neurobiology Research
- Ataxia (Movement) Defects
- Cerebellar Defects
- Neural Tube Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Wnt1^sw/Wnt1^sw
Not Specified

behavior/neurological phenotype

abnormal gait
- affected mice never walk directly forward
- affected mice sway from side-to-side whenever attempting to move forward and lean against the side of the pen for support
- change of direction involves a clockwise or counterclockwise pivot around the rear legs and mice may roll

abnormal locomotor coordination

abnormal posture
- affecte mice never stand normally

ataxia

impaired swimming

weaving

nervous system phenotype

abnormal brain white matter morphology
- white matter is fused directly with the superior colliculi; no meningeal tissue separates them

abnormal cerebellar cortex morphology
- at least by birth cortical tissue is broken into irregular islands of cells crossed by aberrant fascicles of white matter
- despite gross morphology individual cell types are relatively normal

abnormal cerebellum anterior vermis morphology
- loss of the most anterior parts

abnormal cerebellum development
- affected mice retain the sagittal grooves that separate the hemispheres before 15-16 days of embryonic development
- affects the midline parts of the cerebellum
- there is failure of cerebellar hemisphere midline fusion

abnormal inferior colliculus morphology
- lateral displacement of this structure

muscle phenotype

muscle hypertonia
- of limbs

The following phenotype relates to a compound genotype created using this strain

Genotype: Wnt1^sw/Wnt1^sw
B6C3Fe a/a-Wnt1/J

hearing/vestibular/ear phenotype

reduced linear vestibular evoked potential
- elevated threshold and absent central response peaks

Genotype: Wnt1^sw/Wnt1^sw
involves: B6C3Fe * C57BL/6J

nervous system phenotype

absent midbrain-hindbrain boundary
- the boundary between mesencephalon and metencephalon does not form

References

Additional References

Louvi A; Alexandre P; Metin C; Wurst W; Wassef M. 2003. The isthmic neuroepithelium is essential for cerebellar midline fusion. Development 130(22):5319-30PubMed: 14507778 MGI: J:85727
Thomas KR; Musci TS; Neumann PE; Capecchi MR. 1991. Swaying is a mutant allele of the proto-oncogene Wnt-1. Cell 67(5):969-76PubMed: 1835670 MGI: J:2964

Additional - a related

Additional - Wnt1^sw related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the B6C3Fe a/a-Wnt1^sw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000243 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for a,Heterozygous or Homozygous for Wnt1<sw>, 1 pair minimum

Related Products and Services

Frozen Mouse Embryo	B6C3Fe a/a-Wnt1<sw>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6C3Fe a/a-Wnt1<sw>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6C3Fe a/a-Wnt1<sw>/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	B6C3Fe a/a-Wnt1<sw>/J Frozen Embryos	$3373.50

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Genetic overview

Research Applications

Base Price

Detailed Description

Development

a

Allele Symbol: a

Wnt1sw

Allele Symbol: Wnt1sw

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Wnt1sw related

Mammalian Phenotype Terms by Genotype

Genotype: Wnt1sw/Wnt1swNot Specified

behavior/neurological phenotype

nervous system phenotype

muscle phenotype

Genotype: Wnt1sw/Wnt1swB6C3Fe a/a-Wnt1/J

hearing/vestibular/ear phenotype

Genotype: Wnt1sw/Wnt1swinvolves: B6C3Fe * C57BL/6J

nervous system phenotype

References

Additional References

Additional - a related

Additional - Wnt1sw related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Wnt1^sw

Allele Symbol: Wnt1^sw

Genotype: Wnt1^sw related

Genotype: Wnt1^sw/Wnt1^sw
Not Specified

Genotype: Wnt1^sw/Wnt1^sw
B6C3Fe a/a-Wnt1/J

Genotype: Wnt1^sw/Wnt1^sw
involves: B6C3Fe * C57BL/6J

Additional - Wnt1^sw related