Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.
Mice homozygous for the osteopetrosis spontaneous mutation (Csf1op) may be useful to study the role of glia in neurological disease.
Genetic Background | Generation |
---|---|
N81
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Csf1 | colony stimulating factor 1 (macrophage) |
Starting at:
$278.00 Domestic price for female |
483.90 Domestic price for breeder pair |
$2,854.50 Domestic price Cryo Recovery |
Mice homozygous for the osteopetrosis spontaneous mutation (Csf1op) are viable and exhibit osteopetrosis. The osteoclasts are the primary cell type affected in homozygous mutant mice. This results in a generalized macrophage deficiency, monocytopenia, and defective bone remodeling. Homozygous mutant mice also have abnormal calcium regulation, impaired dental growth and female mice fail to lactate. Total leukocyte counts are reduced and marrow cells are decreased to one-tenth of normal control mice. Homozygous mutant mice have a deficient microglia and macrophage response, and therefore may be useful tools to study the role of glia in neurological disease if mated to transgenic models of neurodegenerative disease.
Homozygous pups are identifiable by their phenotype at 10 days of age by absence of incisors and by a domed skull. Unfortunately, ~50% of homozygotes die around weaning age. If they are to survive the weaning process, they must be provided with crushed food in the bottom of the cage. A soft rodent diet could also be used. Those that survive weaning may live up to 6 months.
The osteopetrotic mutation (Csf1op) arose spontaneously in 1970 at The Jackson Laboratory in the beginning congenic stock that became B6.DW-Pou1f1dw/J, which was then at generation N3. Osteopetrotic heterozygotes were backcrossed to C57BL/6J for 7 generations. The line was then bred once to C3FeLe.B6-a/a/J and the strain was subsequently maintained via homozygous ovarian transplant host bred to (C57BL/6J x C3FeLe.B6-a/a/J)F1 then intercross of the obligate heterozygous offspring. Since 2010, The Jackson Laboratory live colony has been maintained by breeding mice heterozygous for Csf1op to B6C3FeF1/J a/a mice (Stock No. 001022).
Allele Name | nonagouti |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 2 |
General Note | Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039 |
Molecular Note | Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type. |
Allele Name | osteopetrosis |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | Csf1- op; Csf-1op; Csfmop; csfmop; M-; op |
Gene Symbol and Name | Csf1, colony stimulating factor 1 (macrophage) |
Gene Synonym(s) | |
Strain of Origin | B6;DW-Pou1f1dw |
Chromosome | 3 |
Molecular Note | A single nucleotide (T) insertion 262 bp downstream from the initiation codon resulted in a frameshift and the creation of a stop codon 21 bp downstream of the insertion. |
Since 2010, The Jackson Laboratory live colony has been maintained by breeding mice heterozygous for Csf1op to B6C3FeF1/J a/a mice (Stock No. 001022).
When using the osteopetrosis mouse strain in a publication, please cite the originating article(s) and include JAX stock #000231 in your Materials and Methods section.
Service/Product | Description | Price |
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Homozygous for a, Heterozygous or Wild-type for Csf1<op> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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