Mutations at the Kit locus affect various aspects of hematopoiesis, the proliferation and migration of primordial germ cells and melanoblasts during development. The KitW-42J mutation has been analyzed as a G to A transition (position 2369) resulting in a missense mutation. Kit receptor tyrosine kinase activity is affected but not c-kit encoded proteins. The original KitW mutation and KitW-42J are lethal mutations when homozygous.
Embryos were cryopreserved in 1980 by mating heterozygous males to C57BL/6J females.
|Allele Name||dominant spotting 42 Jackson|
|Allele Synonym(s)||We; W-42|
|Gene Symbol and Name||Kit, KIT proto-oncogene receptor tyrosine kinase|
|Strain of Origin||C57BL/6J|
|Molecular Note||Nucleotide sequence analysis of mutant complementary DNAs revealed a G-to-A transition (coding nucleotide 2368 in NM_021099) resulting in a aspartic acid to asparagine mutation at codson 790 (p.D790N). Kit receptor tyrosine kinase activity is affected but not c-kit encoded proteins.|
When using the dominant spotting 42 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000127 in your Materials and Methods section.