Overview

Also Known As: dominant spotting 44 Jackson, W⁴⁴

These mice carry a spontaneous remutation at the Kit locus characterized by reduced fertility, normal blood parameters, and minimal white belly spotting.

Genetic overview

Genetic Background	Generation
000664 C57BL/6J

Kit^W-44J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Kit	KIT proto-oncogene receptor tyrosine kinase

View Genetics

Research Applications

Cancer Research
Dermatology Research
Developmental Biology Research
Endocrine Deficiency Research
Reproductive Biology Research
Mouse/Human Gene Homologs
Research Tools
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Kit^W-44J heterozygotes have white-tipped feet and a white tail tip although the belly spot standardly found in Kit^W* mutations is very small in this mutant, sometimes restricted to only a few hairs. Homozygotes have a flecked pelt that is predominantly white, especially ventrally, with pigmented patches particularly at the lateral borders. The pigmentation fades with age yielding black-eyed white mice by approximately 9 months of age. While many Kit^W-44J homozygotes are viable, fertility is diminished. Homozygous females have greatly reduced fertility and gonads that are smaller in size with reduced activity. Homozygous males are sterile although spermatogenesis occurs. The Kit^W-44J allele does not produce anemia in either its heterozygous or homozygous state. The red blood cell count, white blood cell count, hematocrit, and mean cell volume are normal. However, bone marrow transplantation experiments reveal that the colony forming units in the bone marrow of Kit^W-44J homozygotes are much lower than normal despite the fact that the bone marrow has a normal cell count. While bone marrow transplantation from Kit^W-44J homozygotes can cure anemia in Kit^W/Kit^W-v hosts, it repopulates more slowly than does wild type bone marrow. Bone marrow from Kit^W-44J homozygotes also fails to repopulate the mast cells in the skin of Kit^W/Kit^W-v hosts. Wild type bone marrow transplanted into non-irradiated hosts out-competes the endogenous erythropoeisis in Kit^W-44J homozygotes but not in wild type hosts, further confirming a deficiency in erythropoeisis in these non-anemic mice. Mice doubly heterozygous for Kit^W-44J and Kit^W-39J are more mildly anemic than mice doubly heterozygous for Kit^W-39J and other second Kit^W* alleles. (Geissler et al., 1981; Geissler and Russell, 1983; Sawada et al., 1991.)

Development

The Kit^W-44J mutation arose spontaneously at the Jackson Laboratory on the C3H/HeJ background in the mid-1970s. It was backcrossed to C57BL/6J and in 1980 heterozygous males at N33 were bred with C57BL/6J females to produce embryos for cryopreservation. (Geissler et al., 1981 and 1988.)

Genetics

Kit^W-44J

Allele Symbol: Kit^W-44J

Allele Name	dominant spotting 44 Jackson
Allele Type	Spontaneous
Allele Synonym(s)	dominant spotting 44 Jackson; Kit^W-44J
Gene Symbol and Name	Kit, KIT proto-oncogene receptor tyrosine kinase
Gene Synonym(s)	C-Kit; belly-spot; Dominant white spotting; dominant spotting; Gsfsco1; Gsfsco5; Gsfsow3; Gsfsco1; Gsfsco5; Gsfsow3; gsf spotted coat 5; Fdc; PBT; gsf spotted coat 1; Ssm; SCFR; SCO1; SCO5; SOW3; W; spotted sterile male; Steel Factor Receptor; belly-spot; Tr-kit; Bs; phenotype like Sl or W 3; CD117; c-KIT; MASTC
Strain of Origin	C3H/HeJ
Chromosome	5
Molecular Note	Southern hybridization analyses of genomic DNA using probes corresponding to two specific lengths of amino acids from c-kit suggests that this allele comprises a 4-5 kb insertion that disrupts c-kit. The level of c-kit mRNA in homozygotes is markedly reduced.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Kit^W-44J related

Cancer Research
- Growth Factors/Receptors/Cytokines
- Oncogenes
- Increased Tumor Incidence
  - Gonadal Tumors: ovarian
  - Gonadal Tumors
Dermatology Research
- Color and White Spotting Defects
Developmental Biology Research
- Neural Crest Defects
Endocrine Deficiency Research
- Bone/Bone Marrow Defects
- Gonad Defects
- Skin Defects
Reproductive Biology Research
- Gonadal Tumors
  - ovarian
- Developmental Defects Affecting Gonads
  - germ cell deficient
- Fertility Defects
Mouse/Human Gene Homologs
- piebaldism
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - Mast Cell Deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - Mast Cell Deficiency
Neurobiology Research
- Receptor Defects
- Hearing Defects
Sensorineural Research
- Hearing Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Kit^W-44J/Kit^W-44J
B6.C3-Kit/J

endocrine/exocrine gland phenotype

abnormal ovarian follicle number
- ovaries contain fewer developing follicles than in wild-type mice

abnormal ovary morphology
- pathological invagination of the germinal epithelium by 2 months of age

small ovary

integument phenotype

irregular coat pigmentation
- mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders

mammalian phenotype

hematopoietic system phenotype
- Normal - no hematopoietic system abnormalities detected

pigmentation phenotype

irregular coat pigmentation
- mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders

reproductive system phenotype

abnormal ovarian follicle number
- ovaries contain fewer developing follicles than in wild-type mice

abnormal ovary morphology
- pathological invagination of the germinal epithelium by 2 months of age

decreased litter size
- some female mice produce small litters

male infertility
- testes are less than a third normal size and devoid of spermatogenic activity

reduced female fertility
- 50% are fertile and these produce one or two small litters

small ovary

Genotype: Kit^W-44J/Kit⁺
B6.C3-Kit/J

integument phenotype

belly spot
- very small spot to only a few hairs

white spotting
- white feet and tail tip

mammalian phenotype

hematopoietic system phenotype
- Normal - no hematopoietic system abnormalities detected

pigmentation phenotype

belly spot
- very small spot to only a few hairs

white spotting
- white feet and tail tip

References

Additional References

Nocka K; Tan JC; Chiu E; Chu TY; Ray P; Traktman P; Besmer P. 1990. Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W. EMBO J 9(6):1805-13PubMed: 1693331 MGI: J:10528
Sawada K; Sakamaki K; Nishimune Y. 1991. Effect of the W mutation, for white belly spot, on testicular germ cell differentiation in mice. J Reprod Fertil 93(2):287-94PubMed: 1686280 MGI: J:1856
Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9PubMed: 1559233 MGI: J:468
Murphy ED. 1972. Hyperplastic and early neoplastic changes in the ovaries of mice after genic deletion of germ cells. J Natl Cancer Inst 48(5):1283-95PubMed: 4337905 MGI: J:5274
Kitamura Y; Kasugai T; Ebi Y; Nomura S. 1991. Fibroblast-dependent differentiation/proliferation of mast cells. Skin Pharmacol 4 Suppl 1:2-7PubMed: 1764248 MGI: J:619
Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61PubMed: 7274658 MGI: J:6571
Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. I. Influence upon hematopoietic stem cells. Exp Hematol 11(6):452-60PubMed: 6352297 MGI: J:7186
Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. II. Effects on mast cell development. Exp Hematol 11(6):461-6PubMed: 6352298 MGI: J:7187
Geissler EN; Ryan MA; Housman DE. 1988. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55(1):185-92PubMed: 2458842 MGI: J:9374

Additional - Kit^W-44J related

Technical Support

Contact Technical Support

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the dominant spotting 44 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000122 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Kit<W-44J>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: dominant spotting 44 Jackson, W44

Genetic overview

Research Applications

Base Price

Detailed Description

Development

KitW-44J

Allele Symbol: KitW-44J

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: KitW-44J related

Mammalian Phenotype Terms by Genotype

Genotype: KitW-44J/KitW-44JB6.C3-Kit/J

endocrine/exocrine gland phenotype

integument phenotype

mammalian phenotype

pigmentation phenotype

reproductive system phenotype

Genotype: KitW-44J/Kit+B6.C3-Kit/J

integument phenotype

mammalian phenotype

pigmentation phenotype

References

Additional References

Additional - KitW-44J related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: dominant spotting 44 Jackson, W⁴⁴

Kit^W-44J

Allele Symbol: Kit^W-44J

Genotype: Kit^W-44J related

Genotype: Kit^W-44J/Kit^W-44J
B6.C3-Kit/J

Genotype: Kit^W-44J/Kit⁺
B6.C3-Kit/J

Additional - Kit^W-44J related