These mice carry a spontaneous remutation at the Kit locus characterized by reduced fertility, normal blood parameters, and minimal white belly spotting.Read More +
KitW-44J heterozygotes have white-tipped feet and a white tail tip although the belly spot standardly found in KitW* mutations is very small in this mutant, sometimes restricted to only a few hairs. Homozygotes have a flecked pelt that is predominantly white, especially ventrally, with pigmented patches particularly at the lateral borders. The pigmentation fades with age yielding black-eyed white mice by approximately 9 months of age. While many KitW-44J homozygotes are viable, fertility is diminished. Homozygous females have greatly reduced fertility and gonads that are smaller in size with reduced activity. Homozygous males are sterile although spermatogenesis occurs. The KitW-44J allele does not produce anemia in either its heterozygous or homozygous state. The red blood cell count, white blood cell count, hematocrit, and mean cell volume are normal. However, bone marrow transplantation experiments reveal that the colony forming units in the bone marrow of KitW-44J homozygotes are much lower than normal despite the fact that the bone marrow has a normal cell count. While bone marrow transplantation from KitW-44J homozygotes can cure anemia in KitW/KitW-v hosts, it repopulates more slowly than does wild type bone marrow. Bone marrow from KitW-44J homozygotes also fails to repopulate the mast cells in the skin of KitW/KitW-v hosts. Wild type bone marrow transplanted into non-irradiated hosts out-competes the endogenous erythropoeisis in KitW-44J homozygotes but not in wild type hosts, further confirming a deficiency in erythropoeisis in these non-anemic mice. Mice doubly heterozygous for KitW-44J and KitW-39J are more mildly anemic than mice doubly heterozygous for KitW-39J and other second KitW* alleles. (Geissler et al., 1981; Geissler and Russell, 1983; Sawada et al., 1991.)
The KitW-44J mutation arose spontaneously at the Jackson Laboratory on the C3H/HeJ background in the mid-1970s. It was backcrossed to C57BL/6J and in 1980 heterozygous males at N33 were bred with C57BL/6J females to produce embryos for cryopreservation. (Geissler et al., 1981 and 1988.)
|Allele Name||dominant spotting 44 Jackson|
|Allele Synonym(s)||dominant spotting 44 Jackson; KitW-44J|
|Gene Symbol and Name||Kit, KIT proto-oncogene receptor tyrosine kinase|
|Gene Synonym(s)||C-Kit; belly-spot; Dominant white spotting; dominant spotting; Gsfsco1; Gsfsco5; Gsfsow3; Gsfsco1; Gsfsco5; Gsfsow3; gsf spotted coat 5; Fdc; PBT; gsf spotted coat 1; Ssm; SCFR; SCO1; SCO5; SOW3; W; spotted sterile male; Steel Factor Receptor; belly-spot; Tr-kit; Bs; phenotype like Sl or W 3; CD117; c-KIT; MASTC|
|Strain of Origin||C3H/HeJ|
|Molecular Note||Southern hybridization analyses of genomic DNA using probes corresponding to two specific lengths of amino acids from c-kit suggests that this allele comprises a 4-5 kb insertion that disrupts c-kit. The level of c-kit mRNA in homozygotes is markedly reduced.|
|Heterozygous or Wild-type for Kit<W-44J>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.