Overview

Also Known As:dominant spotting 44 Jackson, W⁴⁴

These mice carry a spontaneous remutation at the Kit locus characterized by reduced fertility, normal blood parameters, and minimal white belly spotting.

Genetic overview

Genetic Background	Generation
000664 C57BL/6J

Kit^W-44J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Kit	KIT proto-oncogene receptor tyrosine kinase

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Research Applications

Cancer Research
Dermatology Research
Developmental Biology Research
Endocrine Deficiency Research
Reproductive Biology Research
Mouse/Human Gene Homologs
Research Tools
Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Kit^W-44J heterozygotes have white-tipped feet and a white tail tip although the belly spot standardly found in Kit^W* mutations is very small in this mutant, sometimes restricted to only a few hairs. Homozygotes have a flecked pelt that is predominantly white, especially ventrally, with pigmented patches particularly at the lateral borders. The pigmentation fades with age yielding black-eyed white mice by approximately 9 months of age. While many Kit^W-44J homozygotes are viable, fertility is diminished. Homozygous females have greatly reduced fertility and gonads that are smaller in size with reduced activity. Homozygous males are sterile although spermatogenesis occurs. The Kit^W-44J allele does not produce anemia in either its heterozygous or homozygous state. The red blood cell count, white blood cell count, hematocrit, and mean cell volume are normal. However, bone marrow transplantation experiments reveal that the colony forming units in the bone marrow of Kit^W-44J homozygotes are much lower than normal despite the fact that the bone marrow has a normal cell count. While bone marrow transplantation from Kit^W-44J homozygotes can cure anemia in Kit^W/Kit^W-v hosts, it repopulates more slowly than does wild type bone marrow. Bone marrow from Kit^W-44J homozygotes also fails to repopulate the mast cells in the skin of Kit^W/Kit^W-v hosts. Wild type bone marrow transplanted into non-irradiated hosts out-competes the endogenous erythropoeisis in Kit^W-44J homozygotes but not in wild type hosts, further confirming a deficiency in erythropoeisis in these non-anemic mice. Mice doubly heterozygous for Kit^W-44J and Kit^W-39J are more mildly anemic than mice doubly heterozygous for Kit^W-39J and other second Kit^W* alleles. (Geissler et al., 1981; Geissler and Russell, 1983; Sawada et al., 1991.)

Development

The Kit^W-44J mutation arose spontaneously at the Jackson Laboratory on the C3H/HeJ background in the mid-1970s. It was backcrossed to C57BL/6J and in 1980 heterozygous males at N33 were bred with C57BL/6J females to produce embryos for cryopreservation. (Geissler et al., 1981 and 1988.)

Genetics

Kit^W-44J

Allele Symbol: Kit^W-44J

Allele Name	dominant spotting 44 Jackson
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Kit, KIT proto-oncogene receptor tyrosine kinase
Gene Synonym(s)
Strain of Origin	C3H/HeJ
Chromosome	5
Molecular Note	Southern hybridization analyses of genomic DNA using probes corresponding to two specific lengths of amino acids from c-kit suggests that this allele comprises a 4-5 kb insertion that disrupts c-kit. The level of c-kit mRNA in homozygotes is markedly reduced.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Kit^W-44J related

Cancer Research
- Growth Factors/Receptors/Cytokines
- Oncogenes
- Increased Tumor Incidence
  - Gonadal Tumors: ovarian
  - Gonadal Tumors
Dermatology Research
- Color and White Spotting Defects
Developmental Biology Research
- Neural Crest Defects
Endocrine Deficiency Research
- Bone/Bone Marrow Defects
- Gonad Defects
- Skin Defects
Reproductive Biology Research
- Gonadal Tumors
  - ovarian
- Developmental Defects Affecting Gonads
  - germ cell deficient
- Fertility Defects
Mouse/Human Gene Homologs
- piebaldism
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - Mast Cell Deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - Mast Cell Deficiency
Neurobiology Research
- Receptor Defects
- Hearing Defects
Sensorineural Research
- Hearing Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Kit^W-44J/Kit^W-44J
B6.C3-Kit/J

endocrine/exocrine gland phenotype

abnormal ovarian follicle number
- ovaries contain fewer developing follicles than in wild-type mice

abnormal ovary morphology
- pathological invagination of the germinal epithelium by 2 months of age

small ovary

integument phenotype

irregular coat pigmentation
- mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders

mammalian phenotype

hematopoietic system phenotype
- Normal - no hematopoietic system abnormalities detected

pigmentation phenotype

irregular coat pigmentation
- mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders

reproductive system phenotype

abnormal ovarian follicle number
- ovaries contain fewer developing follicles than in wild-type mice

abnormal ovary morphology
- pathological invagination of the germinal epithelium by 2 months of age

decreased litter size
- some female mice produce small litters

male infertility
- testes are less than a third normal size and devoid of spermatogenic activity

reduced female fertility
- 50% are fertile and these produce one or two small litters

small ovary

Genotype: Kit^W-44J/Kit⁺
B6.C3-Kit/J

integument phenotype

belly spot
- very small spot to only a few hairs

white spotting
- white feet and tail tip

mammalian phenotype

hematopoietic system phenotype
- Normal - no hematopoietic system abnormalities detected

pigmentation phenotype

belly spot
- very small spot to only a few hairs

white spotting
- white feet and tail tip

References

Additional References

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9PubMed: 1559233 MGI: J:468
Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61PubMed: 7274658 MGI: J:6571
Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. I. Influence upon hematopoietic stem cells. Exp Hematol 11(6):452-60PubMed: 6352297 MGI: J:7186
Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. II. Effects on mast cell development. Exp Hematol 11(6):461-6PubMed: 6352298 MGI: J:7187
Geissler EN; Ryan MA; Housman DE. 1988. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55(1):185-92PubMed: 2458842 MGI: J:9374
Kitamura Y; Kasugai T; Ebi Y; Nomura S. 1991. Fibroblast-dependent differentiation/proliferation of mast cells. Skin Pharmacol 4 Suppl 1:2-7PubMed: 1764248 MGI: J:619
Murphy ED. 1972. Hyperplastic and early neoplastic changes in the ovaries of mice after genic deletion of germ cells. J Natl Cancer Inst 48(5):1283-95PubMed: 4337905 MGI: J:5274
Nocka K; Tan JC; Chiu E; Chu TY; Ray P; Traktman P; Besmer P. 1990. Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W. EMBO J 9(6):1805-13PubMed: 1693331 MGI: J:10528
Sawada K; Sakamaki K; Nishimune Y. 1991. Effect of the W mutation, for white belly spot, on testicular germ cell differentiation in mice. J Reprod Fertil 93(2):287-94PubMed: 1686280 MGI: J:1856

Additional - Kit^W-44J related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the dominant spotting 44 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000122 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wild-type for Kit<W-44J>

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:dominant spotting 44 Jackson, W44

Genetic overview

Research Applications

Base Price

Detailed Description

Development

KitW-44J

Allele Symbol: KitW-44J

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: KitW-44J related

Mammalian Phenotype Terms by Genotype

Genotype: KitW-44J/KitW-44JB6.C3-Kit/J

endocrine/exocrine gland phenotype

integument phenotype

mammalian phenotype

pigmentation phenotype

reproductive system phenotype

Genotype: KitW-44J/Kit+B6.C3-Kit/J

integument phenotype

mammalian phenotype

pigmentation phenotype

References

Additional References

Additional - KitW-44J related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:dominant spotting 44 Jackson, W⁴⁴

Kit^W-44J

Allele Symbol: Kit^W-44J

Genotype: Kit^W-44J related

Genotype: Kit^W-44J/Kit^W-44J
B6.C3-Kit/J

Genotype: Kit^W-44J/Kit⁺
B6.C3-Kit/J

Additional - Kit^W-44J related