Mice homozygous for the shaker 2 spontaneous mutation (Myo15sh2) are viable and fertile, showing only a slight reduction in both compared to wildtype mice. Homozygous mutant mice display a phenotype very similar to the behavior and pathology to shaker-1 (Myo7ash1) with the exception that the abnormalities are observed a little earlier in shaker 2 mice. Homozygotes appear to be deaf from the beginning, and the saccular macula is abnormal at birth. The stria vascularis appears normal by light microscopy at 2 weeks, but begins to show degenerative changes shortly thereafter. Many of the cells contain electron-dense inclusions filled with a fine granular material. At 2.5 months, the type I hair cells of the cristae ampullares and maculae utriculi contain rod-shaped inclusion bodies composed of actin filaments. The visible phenotype of the shaker-2 Jackson (Myo15sh2-J, Stock No. 002048) doe...Read More +
Mice homozygous for the shaker 2 spontaneous mutation (Myo15sh2) are viable and fertile, showing only a slight reduction in both compared to wildtype mice. Homozygous mutant mice display a phenotype very similar to the behavior and pathology to shaker-1 (Myo7ash1) with the exception that the abnormalities are observed a little earlier in shaker 2 mice. Homozygotes appear to be deaf from the beginning, and the saccular macula is abnormal at birth. The stria vascularis appears normal by light microscopy at 2 weeks, but begins to show degenerative changes shortly thereafter. Many of the cells contain electron-dense inclusions filled with a fine granular material. At 2.5 months, the type I hair cells of the cristae ampullares and maculae utriculi contain rod-shaped inclusion bodies composed of actin filaments. The visible phenotype of the shaker-2 Jackson (Myo15sh2-J, Stock No. 002048) does not differ from that of the original shaker 2.
|Allele Name||shaker 2|
|Allele Type||Radiation induced|
|Gene Symbol and Name||Myo15, myosin XV|
|Gene Synonym(s)||DFNB3; MYO15; RGD1561873; sh-2; sh2; shaker 2|
|Strain of Origin||Mixed stock|
|General Note||The Myo15sh2 mutation was discovered by Dobrovolskaia-Zavadskaia (J:15021) in descendants of an irradiated male.|
|Molecular Note||The sequences of all 49 exons of this gene were analyzed. A G to A transition was found in codon 674 within exon 18 that produces a Cys to Tyr substitution that likely removes an actin binding site.|
Myo15sh2/Myo15sh2 males may trample pups due to circling behavior; remove the male from the cage when the female is pregnant.
When using the Shaker 2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #000109 in your Materials and Methods section.
|Homozygous or heterozygous for Myo15<sh2>|
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