129T1/Sv-Oca2⁺ Tyr^c-ch Dnd1^Ter/J

Stock number: 000091
Strain synonyms: 129T1
Research areas: Cancer Research, Dermatology Research, Developmental Biology Research, Mouse/Human Gene Homologs, Reproductive Biology Research

Development

This strain was derived from a 129 line (referred to as 129/Sv-WCP) congenic for dominant spotting (Kit^W) and chinchilla (Tyr^c-ch). The congenic was developed from an F1 male (WC x C57BL/6) backcrossed to a 129/Sv female. At the 8th backcross generation, 20% of the males produced by a heterozygous female developed testicular teratomas. Subsequently, sibling matings were selected for the ability to produce males with a high teratoma incidence and the dominant spotting allele was bred out (Stevens LC, 1973, J Natl Cancer Inst). Teratoma incidence in this strain is now attributed to a combination of the spontaneous mutation Dnd1^Ter and the 129 strain background. By 1972, the colony had reached 20 generations of inbreeding. This strain was obtained from LC Stevens at The Jackson Laboratory and was cryopreserved in 1989.

Allele

Symbol: Oca2⁺
Name: wild type
MGI accession ID: MGI:2383978
Generation method: Not Applicable
Marker symbol: Oca2
Marker name: oculocutaneous albinism II
Chromosome: 7
Strain of origin: Not Applicable

Allele

Symbol: Tyr^c-ch
Name: chinchilla
MGI accession ID: MGI:1855977
Generation method: Spontaneous
Marker symbol: Tyr
Marker name: tyrosinase
Chromosome: 7
Strain of origin: fancier's stock
Molecular note: The mutation in the chinchilla allele was found to be a G-to-A point mutation that results in an amino acid change at position 482 or 464 from alanine to threonine (p.A482T for pre-protein, p.A464T for mature protein).

Allele

Symbol: Dnd1^Ter
Name: teratoma
MGI accession ID: MGI:2158668
Generation method: Spontaneous
Marker symbol: Dnd1
Marker name: DND microRNA-mediated repression inhibitor 1
Chromosome: 18
Strain of origin: 129P3
Molecular note: A single C to T base change created a stop codon at arginine residue 178 (p.R178*) or 190 (p.R190*) depending on the isoform.