B6(Cg)-Tyrc-2J/J, or B6-albino mice, are C57BL/6J mice that carry a mutation in the tyrosinase gene. Pigment is completely absent from skin, hair and eyes in mice homozygous for Tyrc-2J. This unique characteristic makes these mice ideal for creation of novel strains with targeted mutations (gene knockouts). B6-albino mice are used to generate blastocysts that can be used for microinjection of genetically modified C57BL/6J embryonic stem cells. Following implantation and gestation, resulting offspring that are chimeric for the donor embryonic stem cells and the recipient blastocyst are easily identified by coat color. This allows an easy method to create knockout mice on the C57BL/6J genetic background.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Null/Knockout) | Tyr | tyrosinase |
Allele Type | Gene Symbol | Gene Name |
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Not Applicable (Not Specified) | Cox7a2l | cytochrome c oxidase subunit 7A2 like |
Mice homozygous for Tyrc-2J are phenotypically identical to homozygotes for the classic albino allele. Pigment is completely absent from skin, hair and eyes. While Tyr mRNA levels of Tyrc-2J homozygotes are similar to those of wild-type mice, there is virtually no tyrosinase protein present (Le Fur et al. 1996). Both homozygote and heterozygote mice are highly resistant to light damage and exhibit retinal degeneration (increased photoreceptor death) into young adulthood. Degeneration does not continue in adult mice (Bravo-Nuevo et al. 2004).
Tyrc-2J arose in 1970 at The Jackson Laboratory on a C57BL/6J congenic strain carrying Tyrp1b (Chr 4) and Hps1ep (Chr 19) when that congenic was at generation N20. Tyrp1b and Hps1ep were eliminated from the Tyrc-2J line by further backcrossing to C57BL/6J (Lane 1973). It was subsequently indicated (Hughes et al., 2007) that the Tyrc-2J mutation arose in C57BL/6J-derived sequence since Dale1 ES cells, which derived from this strain, were found to have C57BL/6J-derived sequence flanking the Tyr locus at D7Mit350 and D7Mit323.
Allele Name | albino 2 Jackson |
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Allele Type | Spontaneous (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Tyr, tyrosinase |
Gene Synonym(s) | |
Site of Expression | Expressed in melanosomes |
Strain of Origin | B6.Cg-Tyrp1b Hps1ep |
Chromosome | 7 |
Molecular Note | This mutation has a G-to-T base change at coding nucleotide 230 resulting in an amino acid change from arginine to leucine at residue 77 (p.R77L) which lies in the highly conserved DDRE sequence. Nucleotide 230 is at the alternative 5' splice donor site for exon 1 and this allele does not produce the 1a or 1b subset of tyrosinase transcripts but does produce a significant increase in 1c and 1d transcripts. Western blots of homozygous mutant skin extracts demostrate the nearly complete absence of the broad 76-84 kDa band of glycosylated wild-type tyrosinase. No tyrosinase activity was found in hairbulb extracts from homozygous mice. |
Allele Name | short |
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Allele Type | Not Applicable (Not Specified) |
Allele Synonym(s) | SCAF1111 |
Gene Symbol and Name | Cox7a2l, cytochrome c oxidase subunit 7A2 like |
Gene Synonym(s) | |
Strain of Origin | multiple strains |
Chromosome | 17 |
General Note | Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T+ Itpr3tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ. |
Molecular Note | This allele contains a 6 bp microdeletion causing the loss of two amino acids and truncating the protein to 111 amino acids instead of 113 amino acids. It is found in BALB/cAnCrl, BALB/cJ, C57BL/6Cr, C57BL/6JCrl, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NJcl, C57BL/6NHsd, C57BL/6NTac, C57BL/6NJ, B6(CG)-Tyr |
When using the B6(Cg)-Tyrc-2J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000058 in your Materials and Methods section.
Facility Barrier Level Descriptions
RB10 (Maximum) |
AX27 (Maximum) |
EM03 (Maximum) |
Service/Product | Description | Price |
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Homozygous for a and Tyr<c-2J>, 1 pair minimum |
Mouse ES Cells | B6(Cg)-TKyr<c-2J>/J-PRX-B6-albino #1 mES cells | $1095.00 |
Mouse ES Cells | B6(Cg)-TKyr<c-2J>/J-PRX-B6-albino #1 mES cells | $1095.00 |
Mouse ES Cells | B6(Cg)-TKyr<c-2J>/J-PRX-B6-albino #1 mES cells | $1095.00 |
Mouse ES Cells | B6(Cg)-TKyr<c-2J>/J-PRX-B6-albino #1 mES cells | $1095.00 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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